4C7

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$40.00
SKU: 4C7
View product citations for antibody 4C7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: HNF3b / FoxA2
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 47.23 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: FOXA2
Immunogen: Rhodopsin (1-6); recombinant.
Alternate Gene Names: HNF-3BETA
Alternate Antibody Name: Fox2A
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q9PWP8 
Immunogen Sequence:
Entrez Gene ID: 395539 
Additional Characterization:
Antibody Registry ID: AB_2278498 
Additional Information:
Recommended Applications: Function Blocking, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C7 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 4C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication

    Cell
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    IF References

    Developmental biology
    Fournier-Thibault C
    Developmental biology 261.2 (2003 Sep 15): 520-36.

    Development (Cambridge, England)
    Arnold HH
    Development (Cambridge, England) 137.24 (2010 Dec): 4249-60.

    Developmental biology
    Wang B
    Developmental biology 430.1 (2017 Oct 1): 156-165.

    WB References

    Molecular and cellular biology
    Costa RH
    Molecular and cellular biology 20.21 (2000 Nov): 8264-82.

    IHC References

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Soula C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.19 (2006 May 10): 5037-48.

    The Journal of biological chemistry
    Kalinichenko VV
    The Journal of biological chemistry 280.23 (2005 Jun 10): 22278-86.

    Gastroenterology
    Peppelenbosch MP
    Gastroenterology 121.2 (2001 Aug): 317-28.

    Development (Cambridge, England)
    Martí E
    Development (Cambridge, England) 135.2 (2008 Jan): 237-47.

    Nucleic acids research
    Stoeckli ET
    Nucleic acids research 39.20 (2011 Nov 1): e133.

    PloS one
    Matise MP
    PloS one 11.9 (2016): e0163267.

    IP References

    Gastroenterology
    Peppelenbosch MP
    Gastroenterology 121.2 (2001 Aug): 317-28.

    The Journal of biological chemistry
    Kalinichenko VV
    The Journal of biological chemistry 280.23 (2005 Jun 10): 22278-86.

    FB References

    Development (Cambridge, England)
    Trembleau A
    Development (Cambridge, England) 130.9 (2003 May): 1867-76.

    Cell
    Vincent JP
    Cell 105.5 (2001 Jun 1): 613-24.

    All References

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Soula C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.19 (2006 May 10): 5037-48.

    The Journal of biological chemistry
    Kalinichenko VV
    The Journal of biological chemistry 280.23 (2005 Jun 10): 22278-86.

    Gastroenterology
    Peppelenbosch MP
    Gastroenterology 121.2 (2001 Aug): 317-28.

    Development (Cambridge, England)
    Martí E
    Development (Cambridge, England) 135.2 (2008 Jan): 237-47.

    Nucleic acids research
    Stoeckli ET
    Nucleic acids research 39.20 (2011 Nov 1): e133.

    PloS one
    Matise MP
    PloS one 11.9 (2016): e0163267.

    Developmental biology
    Fournier-Thibault C
    Developmental biology 261.2 (2003 Sep 15): 520-36.

    Development (Cambridge, England)
    Arnold HH
    Development (Cambridge, England) 137.24 (2010 Dec): 4249-60.

    Developmental biology
    Wang B
    Developmental biology 430.1 (2017 Oct 1): 156-165.

    Molecular and cellular biology
    Costa RH
    Molecular and cellular biology 20.21 (2000 Nov): 8264-82.

    Development (Cambridge, England)
    Trembleau A
    Development (Cambridge, England) 130.9 (2003 May): 1867-76.

    Cell
    Vincent JP
    Cell 105.5 (2001 Jun 1): 613-24.

    Cell
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Hepatology (Baltimore, Md.)
    Costa RH
    Hepatology (Baltimore, Md.) 35.4 (2002 Apr): 790-8.

    American journal of physiology. Lung cellular and molecular physiology
    Costa RH
    American journal of physiology. Lung cellular and molecular physiology 280.4 (2001 Apr): L695-704.

    Development (Cambridge, England)
    Anderson KV
    Development (Cambridge, England) 138.22 (2011 Nov): 4921-30.

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