4C7

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$40.00
SKU: 4C7
View product citations for antibody 4C7 on CiteAb

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Available: 69

DSHB Data Sheet

Catalog Fields

Antigen: HNF3b / FoxA2
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 47.23 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: FOXA2
Immunogen: Rhodopsin (1-6); recombinant.
Alternate Gene Names: HNF-3BETA
Alternate Antibody Name: Fox2A
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q9PWP8 
Immunogen Sequence:
Entrez Gene ID: 395539 
Additional Characterization:
Antibody Registry ID: AB_2278498 
Additional Information:
Recommended Applications: Function Blocking, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C7 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 4C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References

    Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
    Peppelenbosch MP
    Gastroenterology 121.2 (2001 Aug): 317-28.

    The forkhead box m1 transcription factor is essential for embryonic development of pulmonary vasculature.
    Kalinichenko VV
    The Journal of biological chemistry 280.23 (2005 Jun 10): 22278-86.

    FB References
    All References

    Ventral neural progenitors switch toward an oligodendroglial fate in response to increased Sonic hedgehog (Shh) activity: involvement of Sulfatase 1 in modulating Shh signaling in the ventral spinal cord.
    Soula C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.19 (2006 May 10): 5037-48.

    The forkhead box m1 transcription factor is essential for embryonic development of pulmonary vasculature.
    Kalinichenko VV
    The Journal of biological chemistry 280.23 (2005 Jun 10): 22278-86.

    Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
    Peppelenbosch MP
    Gastroenterology 121.2 (2001 Aug): 317-28.

    Wnt canonical pathway restricts graded Shh/Gli patterning activity through the regulation of Gli3 expression.
    Martí E
    Development (Cambridge, England) 135.2 (2008 Jan): 237-47.

    Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development.
    Stoeckli ET
    Nucleic acids research 39.20 (2011 Nov 1): e133.

    Tcf7l2/Tcf4 Transcriptional Repressor Function Requires HDAC Activity in the Developing Vertebrate CNS.
    Matise MP
    PloS one 11.9 (2016): e0163267.

    A dual role for Sonic hedgehog in regulating adhesion and differentiation of neuroepithelial cells.
    Fournier-Thibault C
    Developmental biology 261.2 (2003 Sep 15): 520-36.

    The transcription factors Nkx2.2 and Nkx2.9 play a novel role in floor plate development and commissural axon guidance.
    Arnold HH
    Development (Cambridge, England) 137.24 (2010 Dec): 4249-60.

    Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse.
    Wang B
    Developmental biology 430.1 (2017 Oct 1): 156-165.

    Elevated levels of hepatocyte nuclear factor 3beta in mouse hepatocytes influence expression of genes involved in bile acid and glucose homeostasis.
    Costa RH
    Molecular and cellular biology 20.21 (2000 Nov): 8264-82.

    Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors.
    Trembleau A
    Development (Cambridge, England) 130.9 (2003 May): 1867-76.

    Regulated endocytic routing modulates wingless signaling in Drosophila embryos.
    Vincent JP
    Cell 105.5 (2001 Jun 1): 613-24.

    Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity.
    Jessell TM
    Cell 87.4 (1996 Nov 15): 661-73.

    Maintaining HNF6 expression prevents AdHNF3beta-mediated decrease in hepatic levels of Glut-2 and glycogen.
    Costa RH
    Hepatology (Baltimore, Md.) 35.4 (2002 Apr): 790-8.

    Differential expression of forkhead box transcription factors following butylated hydroxytoluene lung injury.
    Costa RH
    American journal of physiology. Lung cellular and molecular physiology 280.4 (2001 Apr): L695-704.

    Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube.
    Anderson KV
    Development (Cambridge, England) 138.22 (2011 Nov): 4921-30.

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