GN-ID4
Catalog Fields
Antigen: insulin (proinsulin; C-peptide)
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Madsen, O.D.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Human, Monkey
Depositors Notes: This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.
Antigen Molecular Weight: Predicted: 9.4 kDa (proinsulin) and 3 kDa (C-peptide)
Human Protein Atlas:
Predicted Species Reactivity:
Gene: INS
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: C-peptide (aa 40-45 and 57-63 of proinsulin)
Uniprot ID: P01308
Immunogen Sequence: Full length protein
Entrez Gene ID: 3630
Additional Characterization:
Antibody Registry ID: AB_2255626
Additional Information: This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GN-ID4 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GN-ID4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
Steiner DF
Endocrinology 113.6 (1983 Dec): 2135-44.