GN-ID4

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$40.00
SKU: GN-ID4
View product citations for antibody GN-ID4 on CiteAb

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Available: 285

DSHB Data Sheet

Catalog Fields

Antigen: insulin (proinsulin; C-peptide)
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Madsen, O.D.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Human, Monkey
Depositors Notes: This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.
Antigen Molecular Weight: Predicted: 9.4 kDa (proinsulin) and 3 kDa (C-peptide)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: INS
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: C-peptide (aa 40-45 and 57-63 of proinsulin)
Uniprot ID: P01308 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3630 
Additional Characterization:
Antibody Registry ID: AB_2255626 
Additional Information: This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GN-ID4 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GN-ID4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Role of BMP signaling in pancreatic progenitor differentiation from human embryonic stem cells.
    Mfopou JK
    Stem cell reviews and reports 9.5 (2013 Oct): 569-77.

    Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species.
    Kieffer TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 63.8 (2015 Aug): 646-62.

    Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
    Rice CM
    Cell 172.3 (2018 Jan 25): 423-438.e25.

    IHC References

    Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo.
    Steiner DF
    Proceedings of the National Academy of Sciences of the United States of America 85.18 (1988 Sep): 6652-6.

    Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion.
    Madsen OD
    Diabetes 36.10 (1987 Oct): 1203-11.

    Polyclonal origin of pancreatic islets in aggregation mouse chimaeras.
    Jami J
    Development (Cambridge, England) 112.4 (1991 Aug): 1115-21.

    Ocular angiostrongyliasis in Semarang, Central Java.
    Margono SS
    The American journal of tropical medicine and hygiene 26.1 (1977 Jan): 72-4.

    IP References
    FACS References
    Epitope Map References
    All References

    Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo.
    Steiner DF
    Proceedings of the National Academy of Sciences of the United States of America 85.18 (1988 Sep): 6652-6.

    Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion.
    Madsen OD
    Diabetes 36.10 (1987 Oct): 1203-11.

    Polyclonal origin of pancreatic islets in aggregation mouse chimaeras.
    Jami J
    Development (Cambridge, England) 112.4 (1991 Aug): 1115-21.

    Ocular angiostrongyliasis in Semarang, Central Java.
    Margono SS
    The American journal of tropical medicine and hygiene 26.1 (1977 Jan): 72-4.

    Role of BMP signaling in pancreatic progenitor differentiation from human embryonic stem cells.
    Mfopou JK
    Stem cell reviews and reports 9.5 (2013 Oct): 569-77.

    Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species.
    Kieffer TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 63.8 (2015 Aug): 646-62.

    Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
    Rice CM
    Cell 172.3 (2018 Jan 25): 423-438.e25.

    The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
    Steiner DF
    Endocrinology 113.6 (1983 Dec): 2135-44.

    Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells.
    Nostro MC
    Methods (San Diego, Calif.) 101. (2016 May 15): 56-64.

    Human insulin gene expression in transgenic mice: mutational analysis of the regulatory region.
    Bucchini D
    Differentiation; research in biological diversity 60.5 (1996 Sep): 309-16.

    Expression of a human insulin transgene in cholinergic neurons of the mouse medial habenula.
    Calas A
    Biology of the cell 85.2-3 (1995): 137-46.

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