Clone ID / Name: GN-ID4
Anti - insulin (proinsulin; C-peptide)
IF of human pancreatic islet of Langerhans stained with DSHB antibody F55A10 against Nkx6-1(magenta), DSHB antibody GN-ID4 against insulin(green), DAPI(blue). Image credit: M. Hunckler, Gracia Lab
Catalog Fields
Identity
Clone ID / Name: GN-ID4
Alternate Antibody Name: —
Antigen Name: insulin (proinsulin; C-peptide)
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: INS
Entrez Gene ID: 3630
Antigen Sequence: —
Antigen Species: Human
Antigen Mol. Weight: Predicted: 9.4 kDa (proinsulin) and 3 kDa (C-peptide)
Uniprot ID: P01308
Human Protein Atlas: —
Antibody Properties
Host Species: Rat
Clonality: Monoclonal
Isotype: Rat IgG2a
Myeloma Strain: SP2/0-Ag14
Antibody Registry / RRID: AB_2255626
Available to For-Profits: Yes
Hybridoma Available: No
Immunogen & Epitope
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: C-peptide (aa 40-45 and 57-63 of proinsulin)
Clone: —
Additional Info: This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Human, Monkey
Predicted Reactivity: —
Depositor
Depositor Name: O.D. Madsen
Institution: Hagedorn Research Institute
Deposit Date: January 23, 2006
Depositor Notes: This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.
Recommended Applications
FACS
IF
IHC
IP
Each lab must optimize concentration per application and species. Full usage recommendations →
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