GN-ID4
Catalog Fields
Antigen: insulin (proinsulin; C-peptide)
Depositor: Madsen, O.D.
Antigen Species: Human
Depositors Institution: Hagedorn Research Institute
Isotype: RIgG2a
Depositors Notes: This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.
Host Species: rat
Gene: INS
Positive Tested Species Reactivity: Human, Monkey
Clonality: Monoclonal
Antigen Molecular Weight: Predicted: 9.4 kDa (proinsulin) and 3 kDa (C-peptide)
Epitope Mapped: Yes
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Epitope Location or Sequence: C-peptide (aa 40-45 and 57-63 of proinsulin)
Myeloma Strain: SP2/0-Ag14
Immunogen Sequence: Full length protein
Uniprot ID: P01308
Additional Information: This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.
Entrez Gene ID: 3630
Antibody Registry ID: AB_2255626
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GN-ID4 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GN-ID4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
Steiner DF
Endocrinology 113.6 (1983 Dec): 2135-44.