GN-ID4
Catalog Fields
Antigen: insulin (proinsulin; C-peptide)
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Madsen, O.D.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Human, Monkey
Depositors Notes: This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.
Predicted Species Reactivity:
Gene: INS
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: C-peptide (aa 40-45 and 57-63 of proinsulin)
Uniprot ID: P01308
Immunogen Sequence: Full length protein
Entrez Gene ID: 3630
Antibody Registry ID: AB_2255626
Additional Information: This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GN-ID4 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GN-ID4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
Steiner DF
Endocrinology 113.6 (1983 Dec): 2135-44.