Antigen: cadherin HMR-1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works well on C. elegans whole mounts, but doesn't work well for immunoblotting.
Antigen Molecular Weight: Multiple isoforms with a predicted MW of 101, 116, 129, 139, and 324 kDa; Apparent: 130 kDa
Predicted Species Reactivity:
Immunogen: Recombinant His6-tagged HMR-1 (C-terminal 124 aa) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 8H10
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: C-terminal 124 aa
Uniprot ID: Q967F4
Immunogen Sequence: Partial protein
Entrez Gene ID: 173007
Antibody Registry ID: AB_2153752
Additional Information: The Cadherin-related hmr-1 protein is a member of the classical cadherin family of cell adhesion molecules. hmr-1 is expressed in many tissues and timed during development; among other places it is highly expressed in apical junctions of hypodermal cells during embryonic development and later additionally becomes localized to the apical margins of the intestine and pharynx.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HMR1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product HMR1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Epitope Map References