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DSHB Data Sheet

Catalog Fields

Antigen: ERM-1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works well on C. elegans whole mounts. Stains apical membranes.
Antigen Molecular Weight: Predicted: 66 kDa; Apparent: 72 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: erm-1
Immunogen: Recombinant His6-tagged HMR-1 (C-terminal aa 209-563) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 5G2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: C-terminal aa 209-563
Uniprot ID: G5EES2 
Immunogen Sequence: Partial protein
Entrez Gene ID: 172174 
Additional Characterization:  
Antibody Registry ID: AB_10584795 
Additional Information: ERM-1 is a homolog of the ezrin-radixin-moesin (ERM) family of cytoskeletal linker proteins. It localizes most prominently to the apical membranes in the polarized alimentary tract, but is also found on the apical cell cortex of many cells during development.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ERM1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product ERM1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References
    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

    Arp2/3 promotes junction formation and maintenance in the Caenorhabditis elegans intestine by regulating membrane association of apical proteins.
    Soto MC
    Molecular biology of the cell 22.16 (2011 Aug 15): 2886-99.

    A novel function for the MAP kinase SMA-5 in intestinal tube stability.
    Leube RE
    Molecular biology of the cell 27.24 (2016 Dec 1): 3855-3868.

    IHC References
    ELISA References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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