ERM1

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$40.00
SKU: ERM1
View product citations for antibody ERM1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ERM-1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works well on C. elegans whole mounts. Stains apical membranes.
Antigen Molecular Weight: Predicted: 66 kDa; Apparent: 72 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: erm-1
Immunogen: Recombinant His6-tagged HMR-1 (C-terminal aa 209-563) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 5G2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: C-terminal aa 209-563
Uniprot ID: G5EES2 
Immunogen Sequence: Partial protein
Entrez Gene ID: 172174 
Additional Characterization:
Antibody Registry ID: AB_10584795 
Additional Information: ERM-1 is a homolog of the ezrin-radixin-moesin (ERM) family of cytoskeletal linker proteins. It localizes most prominently to the apical membranes in the polarized alimentary tract, but is also found on the apical cell cortex of many cells during development.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ERM1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product ERM1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References
    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

    Arp2/3 promotes junction formation and maintenance in the Caenorhabditis elegans intestine by regulating membrane association of apical proteins.
    Soto MC
    Molecular biology of the cell 22.16 (2011 Aug 15): 2886-99.

    A novel function for the MAP kinase SMA-5 in intestinal tube stability.
    Leube RE
    Molecular biology of the cell 27.24 (2016 Dec 1): 3855-3868.

    IHC References
    ELISA References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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