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View product citations for antibody PYD2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Polychaetoid
Available to For-Profits: Yes
Antigen Species: Drosophila melanogaster
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: Fanning, A.S.
Host Species: mouse
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of North Carolina at Chapel Hill
Antigen Molecular Weight: 165
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Peptide
Gene: PYD
Alternate Antibody Name: UNC9-16.1.3
Alternate Gene Names: Pch, , Pyd/ZO-1, Tamou, ZO-1, ZO1, dzo-1(Z01), tam, xvt, pyd
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3x63-AG8.653
Epitope Mapped: Yes
Uniprot ID: Q9VI04 
Epitope Location or Sequence: aa 587-607
Entrez Gene ID: 41062 
Immunogen Sequence:
Antibody Registry ID: AB_2618043 
Additional Characterization:  
Recommended Applications:  
Additional Information: RRID:AB_2618043
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PYD2 was deposited to the DSHB by Fanning, A.S. (DSHB Hybridoma Product PYD2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
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