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View product citations for antibody PYD2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Polychaetoid
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Fanning, A.S.
Isotype: MIgG2b
Host Species: mouse
Depositors Institution: University of North Carolina at Chapel Hill
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 165
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PYD
Immunogen: Peptide
Alternate Gene Names: Pch, , Pyd/ZO-1, Tamou, ZO-1, ZO1, dzo-1(Z01), tam, xvt, pyd
Alternate Antibody Name: UNC9-16.1.3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63-AG8.653
Epitope Location or Sequence: aa 587-607
Uniprot ID: Q9VI04 
Immunogen Sequence:
Entrez Gene ID: 41062 
Additional Characterization:  
Antibody Registry ID: AB_2618043 
Additional Information: RRID:AB_2618043
Recommended Applications:  
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PYD2 was deposited to the DSHB by Fanning, A.S. (DSHB Hybridoma Product PYD2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
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