Prospero (MR1A)
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Catalog Fields
Antigen: Prospero protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Doe, C.Q.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Oregon
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody recognizes a transcription factor broadly expressed and conserved in vertebrate development.
Antigen Molecular Weight: 165 kDa predicted; 215 kDa observed
Human Protein Atlas:
Predicted Species Reactivity:
Gene: pros
Immunogen: Recombinant protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P29617
Immunogen Sequence:
Entrez Gene ID: 41363
Additional Characterization:
Antibody Registry ID: AB_528440
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Prospero (MR1A) was deposited to the DSHB by Doe, C.Q. (DSHB Hybridoma Product Prospero (MR1A))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
18 References
Initial Publication
RK2, a glial-specific homeodomain protein required for embryonic nerve cord condensation and viability in Drosophila.
Tomlinson A
Development (Cambridge, England) 120.10 (1994 Oct): 2957-66.