Prospero (MR1A)
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Catalog Fields
Clone ID/Product Name: Prospero (MR1A)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: pros
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 41363
Clonality: Monoclonal
Immunogen: GST-fusion protein domain
Clone:
Immunogen Sequence: a.a. 1196-1320
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Prospero protein
Epitope Location or Sequence: Epitope located in C-terminus.
Alternate Antigen Name:
Deposit Date: 11/22/2000
Antigen Molecular Weight: 165 kDa predicted; 215 kDa observed
Depositor: Doe, C.Q.
Antigen Sequence:
Depositor Institution: University of Oregon
Antigen Species: Drosophila
Depositor Notes: This antibody recognizes a transcription factor broadly expressed and conserved in vertebrate development.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Prospero (MR1A) shows no immunoreactivity in embryos homozygous for prospero null alleles. PMID: 7588053.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Prospero (MR1A) was deposited to the DSHB by Doe, C.Q. (DSHB Hybridoma Product Prospero (MR1A))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
22 References
Initial Publication
The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila.
Doe CQ
Development (Cambridge, England) 121.10 (1995 Oct): 3187-95.