Prospero (MR1A)

(0) No Reviews yet
$40.00
SKU: Prospero (MR1A)
View product citations for antibody Prospero (MR1A) on CiteAb

In Stock

Available: 429

DSHB Data Sheet

Catalog Fields

Antigen: Prospero protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Doe, C.Q.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Oregon
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody recognizes a transcription factor broadly expressed and conserved in vertebrate development.
Antigen Molecular Weight: 165 kDa predicted; 215 kDa observed
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: pros
Immunogen: Recombinant protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P29617 
Immunogen Sequence:
Entrez Gene ID: 41363 
Additional Characterization:
Antibody Registry ID: AB_528440 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Prospero (MR1A) was deposited to the DSHB by Doe, C.Q. (DSHB Hybridoma Product Prospero (MR1A))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    klumpfuss distinguishes stem cells from progenitor cells during asymmetric neuroblast division.
    Lee CY
    Development (Cambridge, England) 139.15 (2012 Aug): 2670-80.

    Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development.
    Rebay I
    Mechanisms of development 124.9-10 (2007 Sep-Oct): 792-806.

    Neuralized is essential for a subset of Notch pathway-dependent cell fate decisions during Drosophila eye development.
    Rubin GM
    Proceedings of the National Academy of Sciences of the United States of America 98.10 (2001 May 8): 5637-42.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila.
    Hou SX
    Nature 538.7623 (2016 Oct 6): 109-113.

    Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    WB References
    IHC References

    Replication protein a links cell cycle progression and the onset of neurogenesis in Drosophila optic lobe development.
    Luo H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.7 (2013 Feb 13): 2873-88.

    Notch signaling regulates neuroepithelial stem cell maintenance and neuroblast formation in Drosophila optic lobe development.
    Luo H
    Developmental biology 350.2 (2011 Feb 15): 414-28.

    Drosophila Hey is a target of Notch in asymmetric divisions during embryonic and larval neurogenesis.
    Delidakis C
    Development (Cambridge, England) 137.2 (2010 Jan): 191-201.

    Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development.
    Rebay I
    Mechanisms of development 124.9-10 (2007 Sep-Oct): 792-806.

    Regulated nuclear export of the homeodomain transcription factor Prospero.
    Mortin MA
    Development (Cambridge, England) 128.8 (2001 Apr): 1359-67.

    Revisiting the Drosophila microchaete lineage: a novel intrinsically asymmetric cell division generates a glial cell.
    Schweisguth F
    Development (Cambridge, England) 126.16 (1999 Aug): 3573-84.

    Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila.
    Lu B
    Genes and development 25.24 (2011 Dec 15): 2644-58.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.
    Fulga TA
    Nature communications 8.1 (2017 Nov 21): 1663.

    All References

    Replication protein a links cell cycle progression and the onset of neurogenesis in Drosophila optic lobe development.
    Luo H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.7 (2013 Feb 13): 2873-88.

    Notch signaling regulates neuroepithelial stem cell maintenance and neuroblast formation in Drosophila optic lobe development.
    Luo H
    Developmental biology 350.2 (2011 Feb 15): 414-28.

    Drosophila Hey is a target of Notch in asymmetric divisions during embryonic and larval neurogenesis.
    Delidakis C
    Development (Cambridge, England) 137.2 (2010 Jan): 191-201.

    Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development.
    Rebay I
    Mechanisms of development 124.9-10 (2007 Sep-Oct): 792-806.

    Regulated nuclear export of the homeodomain transcription factor Prospero.
    Mortin MA
    Development (Cambridge, England) 128.8 (2001 Apr): 1359-67.

    Revisiting the Drosophila microchaete lineage: a novel intrinsically asymmetric cell division generates a glial cell.
    Schweisguth F
    Development (Cambridge, England) 126.16 (1999 Aug): 3573-84.

    Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila.
    Lu B
    Genes and development 25.24 (2011 Dec 15): 2644-58.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.
    Fulga TA
    Nature communications 8.1 (2017 Nov 21): 1663.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    klumpfuss distinguishes stem cells from progenitor cells during asymmetric neuroblast division.
    Lee CY
    Development (Cambridge, England) 139.15 (2012 Aug): 2670-80.

    Neuralized is essential for a subset of Notch pathway-dependent cell fate decisions during Drosophila eye development.
    Rubin GM
    Proceedings of the National Academy of Sciences of the United States of America 98.10 (2001 May 8): 5637-42.

    The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila.
    Hou SX
    Nature 538.7623 (2016 Oct 6): 109-113.

    Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    Zfh1 promotes survival of a peripheral glia subtype by antagonizing a Jun N-terminal kinase-dependent apoptotic pathway.
    Garces A
    The EMBO journal 28.20 (2009 Oct 21): 3228-43.

    RK2, a glial-specific homeodomain protein required for embryonic nerve cord condensation and viability in Drosophila.
    Tomlinson A
    Development (Cambridge, England) 120.10 (1994 Oct): 2957-66.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review