2B10

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$40.00
SKU: 2B10
View product citations for antibody 2B10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Cut homeobox
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI / Janelia Farm Research
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is used to stain the tracheal system.
Antigen Molecular Weight: Predicted: 234 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Cut
Immunogen: F2 fusion protein
Alternate Gene Names: Ct; CT; Cut; CUT; Dmel\CG11387;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P10180 
Immunogen Sequence: amino acids 1616-1836 of Cut
Entrez Gene ID: 44540 
Additional Characterization:
Antibody Registry ID: AB_528186 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B10 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 2B10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

56 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Genes & development
    Jan YN
    Genes & development 4.8 (1990 Aug): 1322-31.

    IF References

    PloS one
    Schweisguth F
    PloS one 9.6 (2014): e98213.

    Developmental biology
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    PloS one
    Sun YH
    PloS one 11.9 (2016): e0163744.

    Genesis (New York, N.Y. : 2000)
    Pignoni F
    Genesis (New York, N.Y. : 2000) 54.11 (2016 Nov): 589-592.

    Nature cell biology
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

    PLoS genetics
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Development (Cambridge, England)
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Developmental biology
    Zelhof AC
    Developmental biology 414.1 (2016 Jun 1): 4-20.

    Nature communications
    Schweisguth F
    Nature communications 10.1 (2019 Aug 2): 3486.

    WB References

    Genes & development
    Jan YN
    Genes & development 4.8 (1990 Aug): 1322-31.

    IHC References

    Genes & development
    Jan YN
    Genes & development 4.8 (1990 Aug): 1322-31.

    Development (Cambridge, England)
    Jan YN
    Development (Cambridge, England) 117.2 (1993 Feb): 441-50.

    BMC developmental biology
    Chang HC
    BMC developmental biology 8. (2008 May 8): 50.

    The Journal of cell biology
    Rubinstein E
    The Journal of cell biology 199.3 (2012 Oct 29): 481-96.

    Current biology : CB
    Baker NE
    Current biology : CB 10.15 (2000 Jul 27-Aug 10): 931-4.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    Mechanisms of development
    Skeath JB
    Mechanisms of development 121.12 (2004 Dec): 1495-507.

    Developmental biology
    Dobens LL
    Developmental biology 375.1 (2013 Mar 1): 33-44.

    Developmental cell
    Axelrod JD
    Developmental cell 23.3 (2012 Sep 11): 507-18.

    Journal of genetics and genomics = Yi chuan xue bao
    Lin X
    Journal of genetics and genomics = Yi chuan xue bao 41.7 (2014 Jul 20): 369-78.

    Cell
    Jan YN
    Cell 112.6 (2003 Mar 21): 805-18.

    Neuron
    Uemura T
    Neuron 43.6 (2004 Sep 16): 809-22.

    Current biology : CB
    Baker NE
    Current biology : CB 10.15 (2000 Jul 27-Aug 10): 931-4.

    Development (Cambridge, England)
    Maharaj-Best AC
    Development (Cambridge, England) 140.9 (2013 May): 2039-49.

    Development (Cambridge, England)
    Schüpbach T
    Development (Cambridge, England) 138.9 (2011 May): 1697-703.

    Molecular biology of the cell
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Developmental biology
    Du W
    Developmental biology 335.1 (2009 Nov 1): 228-36.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    Nature cell biology
    Hayashi S
    Nature cell biology 3.9 (2001 Sep): 816-22.

    Development (Cambridge, England)
    Muskavitch MA
    Development (Cambridge, England) 125.22 (1998 Nov): 4531-40.

    Methods (San Diego, Calif.)
    Bray SJ
    Methods (San Diego, Calif.) 68.1 (2014 Jun 15): 173-82.

    Genetics
    LaJeunesse DR
    Genetics 171.3 (2005 Nov): 1125-35.

    Development (Cambridge, England)
    Posakony JW
    Development (Cambridge, England) 138.2 (2011 Jan): 215-25.

    PloS one
    Qu J
    PloS one 9.3 (2014): e92250.

    PLoS genetics
    Audibert A
    PLoS genetics 5.8 (2009 Aug): e1000594.

    The Journal of cell biology
    Schweisguth F
    The Journal of cell biology 188.4 (2010 Feb 22): 581-94.

    Cell
    Grueber WB
    Cell 129.3 (2007 May 4): 593-604.

    Proceedings of the National Academy of Sciences of the United States of America
    Sinha P
    Proceedings of the National Academy of Sciences of the United States of America 110.24 (2013 Jun 11): E2163-72.

    G3 (Bethesda, Md.)
    Zhu AJ
    G3 (Bethesda, Md.) 2.12 (2012 Dec): 1563-75.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Hayward P
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 798-805.

    PloS one
    Bray SJ
    PloS one 8.9 (2013): e75632.

    Journal of biosciences
    Gautam NK
    Journal of biosciences 36.3 (2011 Aug): 531-44.

    BMC developmental biology
    Batterham P
    BMC developmental biology 9. (2009 Dec 9): 64.

    Developmental biology
    Serras F
    Developmental biology 326.1 (2009 Feb 1): 212-23.

    PLoS biology
    Schweisguth F
    PLoS biology 3.4 (2005 Apr): e96.

    Development (Cambridge, England)
    Fortini ME
    Development (Cambridge, England) 140.14 (2013 Jul): 3018-27.

    PLoS genetics
    Fortini ME
    PLoS genetics 9.11 (2013 Nov): e1003917.

    Development (Cambridge, England)
    Posakony JW
    Development (Cambridge, England) 138.2 (2011 Jan): 215-25.

    Development (Cambridge, England)
    Skaer H
    Development (Cambridge, England) 140.5 (2013 Mar): 1100-10.

    Nature cell biology
    Schweisguth F
    Nature cell biology 14.2 (2012 Jan 22): 131-9.

    Genetics
    Rebay I
    Genetics 165.4 (2003 Dec): 1993-2006.

    PloS one
    Cox DN
    PloS one 6.7 (2011): e22611.

    Cell
    Grueber WB
    Cell 129.3 (2007 May 4): 593-604.

    Journal of biosciences
    Gautam NK
    Journal of biosciences 36.3 (2011 Aug): 531-44.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Hayward P
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 798-805.

    Development (Cambridge, England)
    Cohen SM
    Development (Cambridge, England) 122.11 (1996 Nov): 3477-85.

    Proceedings of the National Academy of Sciences of the United States of America
    Sinha P
    Proceedings of the National Academy of Sciences of the United States of America 110.24 (2013 Jun 11): E2163-72.

    Development (Cambridge, England)
    Fortini ME
    Development (Cambridge, England) 140.14 (2013 Jul): 3018-27.

    PLoS genetics
    Fortini ME
    PLoS genetics 9.11 (2013 Nov): e1003917.

    Development (Cambridge, England)
    Posakony JW
    Development (Cambridge, England) 138.2 (2011 Jan): 215-25.

    Development (Cambridge, England)
    Skaer H
    Development (Cambridge, England) 140.5 (2013 Mar): 1100-10.

    Nature cell biology
    Schweisguth F
    Nature cell biology 14.2 (2012 Jan 22): 131-9.

    Genetics
    Rebay I
    Genetics 165.4 (2003 Dec): 1993-2006.

    Genetics
    Moses K
    Genetics 173.2 (2006 Jun): 793-808.

    Proceedings of the National Academy of Sciences of the United States of America
    Raftery LA
    Proceedings of the National Academy of Sciences of the United States of America 105.14 (2008 Apr 8): 5414-9.

    Development (Cambridge, England)
    Calvi BR
    Development (Cambridge, England) 141.1 (2014 Jan): 112-23.

    Nature neuroscience
    Bovolenta P
    Nature neuroscience 14.5 (2011 May): 562-9.

    Development (Cambridge, England)
    Grueber WB
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1351-62.

    Biochimica et biophysica acta. Molecular cell research
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Developmental cell
    Hassan BA
    Developmental cell 50.6 (2019 Sep 23): 780-792.e7.

    Epitope Map References

    Genes & development
    Jan YN
    Genes & development 4.8 (1990 Aug): 1322-31.

    All References

    Genes & development
    Jan YN
    Genes & development 4.8 (1990 Aug): 1322-31.

    Development (Cambridge, England)
    Jan YN
    Development (Cambridge, England) 117.2 (1993 Feb): 441-50.

    BMC developmental biology
    Chang HC
    BMC developmental biology 8. (2008 May 8): 50.

    The Journal of cell biology
    Rubinstein E
    The Journal of cell biology 199.3 (2012 Oct 29): 481-96.

    Current biology : CB
    Baker NE
    Current biology : CB 10.15 (2000 Jul 27-Aug 10): 931-4.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    Mechanisms of development
    Skeath JB
    Mechanisms of development 121.12 (2004 Dec): 1495-507.

    Developmental biology
    Dobens LL
    Developmental biology 375.1 (2013 Mar 1): 33-44.

    Developmental cell
    Axelrod JD
    Developmental cell 23.3 (2012 Sep 11): 507-18.

    Journal of genetics and genomics = Yi chuan xue bao
    Lin X
    Journal of genetics and genomics = Yi chuan xue bao 41.7 (2014 Jul 20): 369-78.

    Cell
    Jan YN
    Cell 112.6 (2003 Mar 21): 805-18.

    Neuron
    Uemura T
    Neuron 43.6 (2004 Sep 16): 809-22.

    Development (Cambridge, England)
    Maharaj-Best AC
    Development (Cambridge, England) 140.9 (2013 May): 2039-49.

    Development (Cambridge, England)
    Schüpbach T
    Development (Cambridge, England) 138.9 (2011 May): 1697-703.

    Molecular biology of the cell
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Developmental biology
    Du W
    Developmental biology 335.1 (2009 Nov 1): 228-36.

    Nature cell biology
    Hayashi S
    Nature cell biology 3.9 (2001 Sep): 816-22.

    Development (Cambridge, England)
    Muskavitch MA
    Development (Cambridge, England) 125.22 (1998 Nov): 4531-40.

    Methods (San Diego, Calif.)
    Bray SJ
    Methods (San Diego, Calif.) 68.1 (2014 Jun 15): 173-82.

    Genetics
    LaJeunesse DR
    Genetics 171.3 (2005 Nov): 1125-35.

    Development (Cambridge, England)
    Posakony JW
    Development (Cambridge, England) 138.2 (2011 Jan): 215-25.

    PloS one
    Qu J
    PloS one 9.3 (2014): e92250.

    PLoS genetics
    Audibert A
    PLoS genetics 5.8 (2009 Aug): e1000594.

    The Journal of cell biology
    Schweisguth F
    The Journal of cell biology 188.4 (2010 Feb 22): 581-94.

    Cell
    Grueber WB
    Cell 129.3 (2007 May 4): 593-604.

    Proceedings of the National Academy of Sciences of the United States of America
    Sinha P
    Proceedings of the National Academy of Sciences of the United States of America 110.24 (2013 Jun 11): E2163-72.

    G3 (Bethesda, Md.)
    Zhu AJ
    G3 (Bethesda, Md.) 2.12 (2012 Dec): 1563-75.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Hayward P
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 798-805.

    PloS one
    Bray SJ
    PloS one 8.9 (2013): e75632.

    Journal of biosciences
    Gautam NK
    Journal of biosciences 36.3 (2011 Aug): 531-44.

    BMC developmental biology
    Batterham P
    BMC developmental biology 9. (2009 Dec 9): 64.

    Developmental biology
    Serras F
    Developmental biology 326.1 (2009 Feb 1): 212-23.

    PLoS biology
    Schweisguth F
    PLoS biology 3.4 (2005 Apr): e96.

    Development (Cambridge, England)
    Fortini ME
    Development (Cambridge, England) 140.14 (2013 Jul): 3018-27.

    PLoS genetics
    Fortini ME
    PLoS genetics 9.11 (2013 Nov): e1003917.

    Development (Cambridge, England)
    Skaer H
    Development (Cambridge, England) 140.5 (2013 Mar): 1100-10.

    Nature cell biology
    Schweisguth F
    Nature cell biology 14.2 (2012 Jan 22): 131-9.

    Genetics
    Rebay I
    Genetics 165.4 (2003 Dec): 1993-2006.

    PloS one
    Cox DN
    PloS one 6.7 (2011): e22611.

    Development (Cambridge, England)
    Cohen SM
    Development (Cambridge, England) 122.11 (1996 Nov): 3477-85.

    Genetics
    Moses K
    Genetics 173.2 (2006 Jun): 793-808.

    Proceedings of the National Academy of Sciences of the United States of America
    Raftery LA
    Proceedings of the National Academy of Sciences of the United States of America 105.14 (2008 Apr 8): 5414-9.

    Development (Cambridge, England)
    Calvi BR
    Development (Cambridge, England) 141.1 (2014 Jan): 112-23.

    Nature neuroscience
    Bovolenta P
    Nature neuroscience 14.5 (2011 May): 562-9.

    Development (Cambridge, England)
    Grueber WB
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1351-62.

    Biochimica et biophysica acta. Molecular cell research
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Developmental cell
    Hassan BA
    Developmental cell 50.6 (2019 Sep 23): 780-792.e7.

    PloS one
    Schweisguth F
    PloS one 9.6 (2014): e98213.

    Developmental biology
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    PloS one
    Sun YH
    PloS one 11.9 (2016): e0163744.

    Genesis (New York, N.Y. : 2000)
    Pignoni F
    Genesis (New York, N.Y. : 2000) 54.11 (2016 Nov): 589-592.

    Nature cell biology
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

    PLoS genetics
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Development (Cambridge, England)
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Developmental biology
    Zelhof AC
    Developmental biology 414.1 (2016 Jun 1): 4-20.

    Nature communications
    Schweisguth F
    Nature communications 10.1 (2019 Aug 2): 3486.

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