Antigen: Cut homeobox
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Host Species: mouse
Depositors Institution: HHMI / Janelia Farm Research
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is used to stain the tracheal system.
Antigen Molecular Weight: Predicted: 234 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: F2 fusion protein
Alternate Gene Names: Ct; CT; Cut; CUT; Dmel\CG11387;
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P10180
Immunogen Sequence: amino acids 1616-1836 of Cut
Entrez Gene ID: 44540
Antibody Registry ID: AB_528186
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B10 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 2B10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References