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View product citations for antibody PYD1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Polychaetoid
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Fanning, A.S.
Isotype: MIgG3
Host Species: mouse
Depositors Institution: Univ. of North Carolina at Chapel Hill
Positive Tested Species Reactivity: Drosophila
Gene: PYD
Antigen Molecular Weight: 165
Alternate Gene Names: Pch, , Pyd/ZO-1, Tamou, ZO-1, ZO1, dzo-1(Z01), tam, xvt, pyd
Immunogen: Peptide
Clonality: Monoclonal
Alternate Antibody Name: UNC11-59.1.1
Epitope Mapped: Yes
Myeloma Strain: P3x63-AG8.653
Epitope Location or Sequence: aa 327-344
Uniprot ID: Q9VI04 
Additional Information: RRID:AB_2618042
Entrez Gene ID: 41062 
Antibody Registry ID: AB_2618042 
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PYD1 was deposited to the DSHB by Fanning, A.S. (DSHB Hybridoma Product PYD1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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