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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: PYD1
Available to For-Profits: Yes
Alternate Antibody Name: UNC11-59.1.1
Gene Symbol: PYD
Ab Isotype: MIgG3
Gene Name:
Antibody Registry ID: AB_2618042 
Uniprot ID: Q9VI04 
Entrez Gene ID: 41062 
Clonality: Monoclonal
Immunogen: Peptide
Immunogen Sequence:
Myeloma Strain: P3x63-AG8.653
Epitope Mapped: Yes
Antigen Name: Polychaetoid
Epitope Location or Sequence: aa 327-344
Alternate Antigen Name:
Deposit Date: 6/3/2014
Antigen Molecular Weight: 165
Depositor: Fanning, A.S.
Depositor Institution: University of North Carolina at Chapel Hill
Antigen Species: Drosophila melanogaster
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_2618042
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications:  
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PYD1 was deposited to the DSHB by Fanning, A.S. (DSHB Hybridoma Product PYD1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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