GL2A7
Catalog Fields
Antigen: LAMP-2 (mouse)
Available to For-Profits: No
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): No
Isotype: RIgG2a, kappa light chain
Depositor: Granger, B.L.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Mouse
Depositors Institution: Yale University
Antigen Molecular Weight: Predicted: 43 kDa; Apparent: 110 kDa
Depositors Notes: Doesn't recognize rat, hamster or primate antigen. GL2A7 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Immunogen: Mouse lysosomes (2% glutaraldehyde fixed) isolated by Percoll gradient fractionation of liver homogenate
Gene: Lamp2
Alternate Antibody Name: CD107b
Alternate Gene Names: CD107b, Mac3, LGP-B, Igp110
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3U1
Epitope Mapped: Yes
Uniprot ID: P17047
Epitope Location or Sequence: Lumenal region
Entrez Gene ID: 16784
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2314734
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GL2A7 was deposited to the DSHB by Granger, B.L. (DSHB Hybridoma Product GL2A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells.
Helenius A
The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.