GL2A7

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$40.00
SKU: GL2A7
View product citations for antibody GL2A7 on CiteAb

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Available: 180

DSHB Data Sheet

Catalog Fields

Antigen: LAMP-2 (mouse)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Granger, B.L.
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Montana State University
Positive Tested Species Reactivity: Mouse
Depositors Notes: Doesn't recognize rat, hamster or primate antigen. GL2A7 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Antigen Molecular Weight: Predicted: 43 kDa; Apparent: 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lamp2
Immunogen: Mouse lysosomes (2% glutaraldehyde fixed) isolated by Percoll gradient fractionation of liver homogenate
Alternate Gene Names: CD107b, Mac3, LGP-B, Igp110
Alternate Antibody Name: CD107b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: Lumenal region
Uniprot ID: P17047 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16784 
Additional Characterization:
Antibody Registry ID: AB_2314734 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GL2A7 was deposited to the DSHB by Granger, B.L. (DSHB Hybridoma Product GL2A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of biological chemistry
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    IF References

    The Journal of biological chemistry
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    Journal of cell science
    Wada Y
    Journal of cell science 119.Pt 21 (2006 Nov 1): 4531-40.

    Journal of immunology (Baltimore, Md. : 1950)
    Webster P
    Journal of immunology (Baltimore, Md. : 1950) 162.2 (1999 Jan 15): 1150-5.

    The Journal of biological chemistry
    Hara-Nishimura I
    The Journal of biological chemistry 278.35 (2003 Aug 29): 33194-9.

    Nature neuroscience
    Cepko CL
    Nature neuroscience 12.1 (2009 Jan): 44-52.

    The Journal of biological chemistry
    Futai M
    The Journal of biological chemistry 275.9 (2000 Mar 3): 6523-9.

    Journal of cell science
    Setty SR
    Journal of cell science 128.17 (2015 Sep 1): 3263-76.

    Cell death & disease
    Punzo C
    Cell death & disease 7.6 (2016 Jun 30): e2279.

    Scientific reports
    Oancea E
    Scientific reports 6. (2016 May 27): 26570.

    The Journal of clinical investigation
    Ginsberg HN
    The Journal of clinical investigation 126.10 (2016 Oct 3): 3852-3867.

    The Journal of cell biology
    Marks MS
    The Journal of cell biology 214.3 (2016 Aug 1): 293-308.

    Occupational health; a journal for occupational health nurses
    Blyth-Bristow B
    Occupational health; a journal for occupational health nurses 41.6 (1989 Jun): 163-5.

    WB References

    The Journal of biological chemistry
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    Journal of immunology (Baltimore, Md. : 1950)
    Webster P
    Journal of immunology (Baltimore, Md. : 1950) 162.2 (1999 Jan 15): 1150-5.

    IP References

    The Journal of biological chemistry
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    Epitope Map References

    The Journal of biological chemistry
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    All References

    The Journal of biological chemistry
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    Journal of cell science
    Wada Y
    Journal of cell science 119.Pt 21 (2006 Nov 1): 4531-40.

    Journal of immunology (Baltimore, Md. : 1950)
    Webster P
    Journal of immunology (Baltimore, Md. : 1950) 162.2 (1999 Jan 15): 1150-5.

    The Journal of biological chemistry
    Hara-Nishimura I
    The Journal of biological chemistry 278.35 (2003 Aug 29): 33194-9.

    Nature neuroscience
    Cepko CL
    Nature neuroscience 12.1 (2009 Jan): 44-52.

    The Journal of biological chemistry
    Futai M
    The Journal of biological chemistry 275.9 (2000 Mar 3): 6523-9.

    Journal of cell science
    Setty SR
    Journal of cell science 128.17 (2015 Sep 1): 3263-76.

    Cell death & disease
    Punzo C
    Cell death & disease 7.6 (2016 Jun 30): e2279.

    Scientific reports
    Oancea E
    Scientific reports 6. (2016 May 27): 26570.

    The Journal of clinical investigation
    Ginsberg HN
    The Journal of clinical investigation 126.10 (2016 Oct 3): 3852-3867.

    The Journal of cell biology
    Marks MS
    The Journal of cell biology 214.3 (2016 Aug 1): 293-308.

    Occupational health; a journal for occupational health nurses
    Blyth-Bristow B
    Occupational health; a journal for occupational health nurses 41.6 (1989 Jun): 163-5.

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