GL2A7

(0) No Reviews yet
$40.00
SKU: GL2A7
View product citations for antibody GL2A7 on CiteAb

In Stock

Available: 180

DSHB Data Sheet

Catalog Fields

Antigen: LAMP-2 (mouse)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Granger, B.L.
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Montana State University
Positive Tested Species Reactivity: Mouse
Depositors Notes: Doesn't recognize rat, hamster or primate antigen. GL2A7 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Antigen Molecular Weight: Predicted: 43 kDa; Apparent: 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lamp2
Immunogen: Mouse lysosomes (2% glutaraldehyde fixed) isolated by Percoll gradient fractionation of liver homogenate
Alternate Gene Names: CD107b, Mac3, LGP-B, Igp110
Alternate Antibody Name: CD107b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: Lumenal region
Uniprot ID: P17047 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16784 
Additional Characterization:
Antibody Registry ID: AB_2314734 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GL2A7 was deposited to the DSHB by Granger, B.L. (DSHB Hybridoma Product GL2A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells.
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    IF References

    Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells.
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    The a3 isoform of V-ATPase regulates insulin secretion from pancreatic beta-cells.
    Wada Y
    Journal of cell science 119.Pt 21 (2006 Nov 1): 4531-40.

    Involvement of MIIC-like late endosomes in B cell receptor-mediated antigen processing in murine B cells.
    Webster P
    Journal of immunology (Baltimore, Md. : 1950) 162.2 (1999 Jan 15): 1150-5.

    Biosynthetic processing of cathepsins and lysosomal degradation are abolished in asparaginyl endopeptidase-deficient mice.
    Hara-Nishimura I
    The Journal of biological chemistry 278.35 (2003 Aug 29): 33194-9.

    Stimulation of the insulin/mTOR pathway delays cone death in a mouse model of retinitis pigmentosa.
    Cepko CL
    Nature neuroscience 12.1 (2009 Jan): 44-52.

    Syntaxin 7 mediates endocytic trafficking to late endosomes.
    Futai M
    The Journal of biological chemistry 275.9 (2000 Mar 3): 6523-9.

    STX13 regulates cargo delivery from recycling endosomes during melanosome biogenesis.
    Setty SR
    Journal of cell science 128.17 (2015 Sep 1): 3263-76.

    TSC but not PTEN loss in starving cones of retinitis pigmentosa mice leads to an autophagy defect and mTORC1 dissociation from the lysosome.
    Punzo C
    Cell death and disease 7.6 (2016 Jun 30): e2279.

    A melanosomal two-pore sodium channel regulates pigmentation.
    Oancea E
    Scientific reports 6. (2016 May 27): 26570.

    Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis.
    Ginsberg HN
    The Journal of clinical investigation 126.10 (2016 Oct 3): 3852-3867.

    BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers.
    Marks MS
    The Journal of cell biology 214.3 (2016 Aug 1): 293-308.

    Implementing a smoking policy at work--a case study.
    Blyth-Bristow B
    Occupational health; a journal for occupational health nurses 41.6 (1989 Jun): 163-5.

    WB References

    Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells.
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    Involvement of MIIC-like late endosomes in B cell receptor-mediated antigen processing in murine B cells.
    Webster P
    Journal of immunology (Baltimore, Md. : 1950) 162.2 (1999 Jan 15): 1150-5.

    IP References

    Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells.
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    Epitope Map References

    Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells.
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    All References

    Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells.
    Helenius A
    The Journal of biological chemistry 265.20 (1990 Jul 15): 12036-43.

    The a3 isoform of V-ATPase regulates insulin secretion from pancreatic beta-cells.
    Wada Y
    Journal of cell science 119.Pt 21 (2006 Nov 1): 4531-40.

    Involvement of MIIC-like late endosomes in B cell receptor-mediated antigen processing in murine B cells.
    Webster P
    Journal of immunology (Baltimore, Md. : 1950) 162.2 (1999 Jan 15): 1150-5.

    Biosynthetic processing of cathepsins and lysosomal degradation are abolished in asparaginyl endopeptidase-deficient mice.
    Hara-Nishimura I
    The Journal of biological chemistry 278.35 (2003 Aug 29): 33194-9.

    Stimulation of the insulin/mTOR pathway delays cone death in a mouse model of retinitis pigmentosa.
    Cepko CL
    Nature neuroscience 12.1 (2009 Jan): 44-52.

    Syntaxin 7 mediates endocytic trafficking to late endosomes.
    Futai M
    The Journal of biological chemistry 275.9 (2000 Mar 3): 6523-9.

    STX13 regulates cargo delivery from recycling endosomes during melanosome biogenesis.
    Setty SR
    Journal of cell science 128.17 (2015 Sep 1): 3263-76.

    TSC but not PTEN loss in starving cones of retinitis pigmentosa mice leads to an autophagy defect and mTORC1 dissociation from the lysosome.
    Punzo C
    Cell death and disease 7.6 (2016 Jun 30): e2279.

    A melanosomal two-pore sodium channel regulates pigmentation.
    Oancea E
    Scientific reports 6. (2016 May 27): 26570.

    Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis.
    Ginsberg HN
    The Journal of clinical investigation 126.10 (2016 Oct 3): 3852-3867.

    BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers.
    Marks MS
    The Journal of cell biology 214.3 (2016 Aug 1): 293-308.

    Implementing a smoking policy at work--a case study.
    Blyth-Bristow B
    Occupational health; a journal for occupational health nurses 41.6 (1989 Jun): 163-5.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review