Clone ID / Name: GL2A7
Anti - LAMP-2 (mouse)
Catalog Fields
Identity
Antibody Properties
Host Species: Rat
Clonality: Monoclonal
Isotype: RIgG2a, kappa light chain
Myeloma Strain: P3U1
Antibody Registry / RRID: AB_2314734
Available to For-Profits: No
Hybridoma Available: No
Immunogen & Epitope
Immunogen: Mouse lysosomes (2% glutaraldehyde fixed) isolated by Percoll gradient fractionation of liver homogenate
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: Lumenal region
Clone: —
Additional Info: —
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Mouse
Predicted Reactivity: —
Depositor
Depositor Name: B.L. Granger
Institution: Yale University
Deposit Date: January 9, 1997
Depositor Notes: Doesn't recognize rat, hamster or primate antigen. GL2A7 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Recommended Applications
IF
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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