anti-delta SA4

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$40.00
SKU: anti-delta SA4
View product citations for antibody anti-delta SA4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: AP-3 (delta subunit)
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Peden, A.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Genentech Inc., 1 DNA Way, South San Francisco
Positive Tested Species Reactivity: Human, Mammal, Mouse
Depositors Notes: Works on all mammalian cell lines tested. Specific to delta adaptin by western blot and immunofluorescence.
Antigen Molecular Weight: Predicted: multiple isoforms between 11 and 130 kDa; Apparent: 160 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: AP3D1
Immunogen: Recombinant GST/delta adaptin (aa 608-800) fusion protein
Alternate Gene Names: ADTD, HPS10, hBLVR
Alternate Antibody Name: SA4
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: Hinge region (aa 770-800 of isoform 1: O14617-1)
Uniprot ID: O14617 
Immunogen Sequence: Partial protein
Entrez Gene ID: 8943 
Additional Characterization:  
Antibody Registry ID: AB_2056641 
Additional Information: The AP-3 complex subunit delta-1 is also known as delta adaptin.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-delta SA4 was deposited to the DSHB by Peden, A.A. (DSHB Hybridoma Product anti-delta SA4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins.
    Klumperman J
    The Journal of cell biology 164.7 (2004 Mar 29): 1065-76.

    Structural basis of the intracellular sorting of the SNARE VAMP7 by the AP3 adaptor complex.
    Owen DJ
    Developmental cell 22.5 (2012 May 15): 979-88.

    Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers.
    Kirchhausen T
    Cell reports 2.5 (2012 Nov 29): 1111-9.

    Differential use of two AP-3-mediated pathways by lysosomal membrane proteins.
    Luzio JP
    Traffic (Copenhagen, Denmark) 5.12 (2004 Dec): 946-62.

    Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain.
    Faundez V
    Molecular biology of the cell 16.1 (2005 Jan): 128-40.

    The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes.
    McCormick PJ
    Traffic (Copenhagen, Denmark) 8.10 (2007 Oct): 1440-51.

    AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.
    Shen J
    Developmental cell 50.4 (2019 Aug 19): 436-446.e5.

    WB References

    Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins.
    Klumperman J
    The Journal of cell biology 164.7 (2004 Mar 29): 1065-76.

    Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis.
    Bergson C
    The Journal of biological chemistry 281.22 (2006 Jun 2): 15182-93.

    Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization.
    Bitoun M
    The Journal of cell biology 205.3 (2014 May 12): 377-93.

    Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.
    Faundez V
    Molecular biology of the cell 24.15 (2013 Aug): 2378-88.

    Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain.
    Faundez V
    Molecular biology of the cell 16.1 (2005 Jan): 128-40.

    AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.
    Shen J
    Developmental cell 50.4 (2019 Aug 19): 436-446.e5.

    IHC References

    Hermansky-Pudlak protein complexes, AP-3 and BLOC-1, differentially regulate presynaptic composition in the striatum and hippocampus.
    Faundez V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.3 (2010 Jan 20): 820-31.

    IP References
    Epitope Map References
    All References

    Hermansky-Pudlak protein complexes, AP-3 and BLOC-1, differentially regulate presynaptic composition in the striatum and hippocampus.
    Faundez V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.3 (2010 Jan 20): 820-31.

    Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins.
    Klumperman J
    The Journal of cell biology 164.7 (2004 Mar 29): 1065-76.

    Structural basis of the intracellular sorting of the SNARE VAMP7 by the AP3 adaptor complex.
    Owen DJ
    Developmental cell 22.5 (2012 May 15): 979-88.

    Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers.
    Kirchhausen T
    Cell reports 2.5 (2012 Nov 29): 1111-9.

    Differential use of two AP-3-mediated pathways by lysosomal membrane proteins.
    Luzio JP
    Traffic (Copenhagen, Denmark) 5.12 (2004 Dec): 946-62.

    Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain.
    Faundez V
    Molecular biology of the cell 16.1 (2005 Jan): 128-40.

    The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes.
    McCormick PJ
    Traffic (Copenhagen, Denmark) 8.10 (2007 Oct): 1440-51.

    AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.
    Shen J
    Developmental cell 50.4 (2019 Aug 19): 436-446.e5.

    Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis.
    Bergson C
    The Journal of biological chemistry 281.22 (2006 Jun 2): 15182-93.

    Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization.
    Bitoun M
    The Journal of cell biology 205.3 (2014 May 12): 377-93.

    Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.
    Faundez V
    Molecular biology of the cell 24.15 (2013 Aug): 2378-88.

    Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles.
    Faundez V
    Molecular biology of the cell 16.8 (2005 Aug): 3692-704.

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