anti-delta SA4
Catalog Fields
Antigen: AP-3 (delta subunit)
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Peden, A.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Genentech Inc.
Positive Tested Species Reactivity: Human, Mammal, Mouse
Depositors Notes: Works on all mammalian cell lines tested. Specific to delta adaptin by western blot and immunofluorescence.
Antigen Molecular Weight: Predicted: multiple isoforms between 11 and 130 kDa; Apparent: 160 kDa
Predicted Species Reactivity:
Gene: AP3D1
Immunogen: Recombinant GST/delta adaptin (aa 608-800) fusion protein
Alternate Gene Names: ADTD, HPS10, hBLVR
Alternate Antibody Name: SA4
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: Hinge region (aa 770-800 of isoform 1: O14617-1)
Uniprot ID: O14617
Immunogen Sequence: Partial protein
Entrez Gene ID: 8943
Antibody Registry ID: AB_2056641
Additional Information: The AP-3 complex subunit delta-1 is also known as delta adaptin.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-delta SA4 was deposited to the DSHB by Peden, A.A. (DSHB Hybridoma Product anti-delta SA4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins.
Klumperman J
The Journal of cell biology 164.7 (2004 Mar 29): 1065-76.