anti-delta SA4
Catalog Fields
Antigen: AP-3 (delta subunit)
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Peden, A.A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mammal, Mouse
Depositors Institution: Genentech Inc.
Antigen Molecular Weight: Predicted: multiple isoforms between 11 and 130 kDa; Apparent: 160 kDa
Depositors Notes: Works on all mammalian cell lines tested. Specific to delta adaptin by western blot and immunofluorescence.
Immunogen: Recombinant GST/delta adaptin (aa 608-800) fusion protein
Gene: AP3D1
Alternate Antibody Name: SA4
Alternate Gene Names: ADTD, HPS10, hBLVR
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: Yes
Uniprot ID: O14617
Epitope Location or Sequence: Hinge region (aa 770-800 of isoform 1: O14617-1)
Entrez Gene ID: 8943
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2056641
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: The AP-3 complex subunit delta-1 is also known as delta adaptin.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-delta SA4 was deposited to the DSHB by Peden, A.A. (DSHB Hybridoma Product anti-delta SA4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins.
Klumperman J
The Journal of cell biology 164.7 (2004 Mar 29): 1065-76.