3CB2
Catalog Fields
Antigen: Vimentin (radial glial and neuronal stem cells)
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: De La Rosa, E.J.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centro de Investigaciones Biologicas CSIC, Madrid
Positive Tested Species Reactivity: Chameleon, Chick, Human, Mouse, Quail, Rat
Depositors Notes: 3CB2 is a cell marker from neural stem cells and radial glia. 3CB2 also recognizes three isoforms of vimentin in gliomas.
Predicted Species Reactivity:
Gene: VIM
Immunogen: E8 optic tectum, fractionated
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Ag8.653
Epitope Location or Sequence:
Uniprot ID: P09654
Immunogen Sequence: Full length sequence
Entrez Gene ID: 420519
Antibody Registry ID: AB_531888
Additional Information: RRID:AB_531888
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3CB2 was deposited to the DSHB by De La Rosa, E.J. (DSHB Hybridoma Product 3CB2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
24 References
Initial Publication
Early expression of a novel radial glia antigen in the chick embryo.
de la Rosa EJ
Glia 15.4 (1995 Dec): 389-400.