3CB2

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$40.00
SKU: 3CB2
View product citations for antibody 3CB2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Vimentin (radial glial and neuronal stem cells)
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: De La Rosa, E.J.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centro de Investigaciones Biológicas CSIC, Madrid
Positive Tested Species Reactivity: Chameleon, Chick, Human, Mouse, Quail, Rat
Depositors Notes: 3CB2 is a cell marker from neural stem cells and radial glia. 3CB2 also recognizes three isoforms of vimentin in gliomas.
Antigen Molecular Weight: 53.1 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: VIM
Immunogen: E8 optic tectum, fractionated
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Ag8.653
Epitope Location or Sequence:
Uniprot ID: P09654 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 420519 
Additional Characterization:
Antibody Registry ID: AB_531888 
Additional Information: RRID:AB_531888
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3CB2 was deposited to the DSHB by De La Rosa, E.J. (DSHB Hybridoma Product 3CB2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Early expression of a novel radial glia antigen in the chick embryo.
    de la Rosa EJ
    Glia 15.4 (1995 Dec): 389-400.

    Vimentin isoform expression in the human retina characterized with the monoclonal antibody 3CB2.
    Prada C
    Journal of neuroscience research 86.8 (2008 Jun): 1871-83.

    Temporal progressive antigen expression in radial glia after contusive spinal cord injury in adult rats.
    Norimatsu H
    Glia 42.2 (2003 Apr 15): 172-83.

    GSK-3beta inhibition/beta-catenin stabilization in ventral midbrain precursors increases differentiation into dopamine neurons.
    Arenas E
    Journal of cell science 117.Pt 24 (2004 Nov 15): 5731-7.

    Matrix metalloproteinases are required for retinal ganglion cell axon guidance at select decision points.
    McFarlane S
    Development (Cambridge, England) 132.15 (2005 Aug): 3371-9.

    Niche-independent symmetrical self-renewal of a mammalian tissue stem cell.
    Smith A
    PLoS biology 3.9 (2005 Sep): e283.

    Identification of two novel glial-restricted cell populations in the embryonic telencephalon arising from unique origins.
    Mayer-Pröschel M
    BMC developmental biology 7. (2007 Apr 17): 33.

    Stable expression of intracellular Notch suppresses v-Src-induced transformation in avian neural cells.
    Calothy G
    Oncogene 26.23 (2007 May 17): 3338-51.

    CD133 (Prominin) negative human neural stem cells are clonogenic and tripotent.
    Smith A
    PloS one 4.5 (2009): e5498.

    Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens.
    Dirks P
    Cell stem cell 4.6 (2009 Jun 5): 568-80.

    Developmental regulation of human embryonic stem cell-derived neurons by calcium entry via transient receptor potential channels.
    Zhang SC
    Stem cells (Dayton, Ohio) 27.12 (2009 Dec): 2906-16.

    Real time imaging of human progenitor neurogenesis.
    Svendsen CN
    PloS one 5.10 (2010 Oct 7): e13187.

    Characterization of the antigenic phenotype of αB-crystallin-expressing peripapillary glial cells in the developing chick retina.
    Seo JH
    Anatomy and cell biology 44.1 (2011 Mar): 35-40.

    Interplay between FGF2 and BMP controls the self-renewal, dormancy and differentiation of rat neural stem cells.
    Smith A
    Journal of cell science 124.Pt 11 (2011 Jun 1): 1867-77.

    Glucose transporter 1 and monocarboxylate transporters 1, 2, and 4 localization within the glial cells of shark blood-brain-barriers.
    Nualart F
    PloS one 7.2 (2012): e32409.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    Effects of High-Temperature-Pressure Polymerized Resin-Infiltrated Ceramic Networks on Oral Stem Cells.
    Fournier BP
    PloS one 11.5 (2016): e0155450.

    A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome.
    Engle EC
    Nature communications 8. (2017 Jul 6): 16077.

    WB References

    Early expression of a novel radial glia antigen in the chick embryo.
    de la Rosa EJ
    Glia 15.4 (1995 Dec): 389-400.

    Vimentin isoform expression in the human retina characterized with the monoclonal antibody 3CB2.
    Prada C
    Journal of neuroscience research 86.8 (2008 Jun): 1871-83.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    IHC References

    Early expression of a novel radial glia antigen in the chick embryo.
    de la Rosa EJ
    Glia 15.4 (1995 Dec): 389-400.

    Vimentin isoform expression in the human retina characterized with the monoclonal antibody 3CB2.
    Prada C
    Journal of neuroscience research 86.8 (2008 Jun): 1871-83.

    Temporal progressive antigen expression in radial glia after contusive spinal cord injury in adult rats.
    Norimatsu H
    Glia 42.2 (2003 Apr 15): 172-83.

    GSK-3beta inhibition/beta-catenin stabilization in ventral midbrain precursors increases differentiation into dopamine neurons.
    Arenas E
    Journal of cell science 117.Pt 24 (2004 Nov 15): 5731-7.

    Wnt2b inhibits differentiation of retinal progenitor cells in the absence of Notch activity by downregulating the expression of proneural genes.
    Nakagawa S
    Development (Cambridge, England) 132.12 (2005 Jun): 2759-70.

    Stage and region dependent expression of a radial glial marker in commissural fibers in kindled mice.
    Itano T
    Epilepsy research 67.1-2 (2005 Oct-Nov): 61-72.

    Human embryonic stem cell derived astrocytes mediate non-cell-autonomous neuroprotection through endogenous and drug-induced mechanisms.
    Chandran S
    Cell death and differentiation 19.5 (2012 May): 779-87.

    Spatial domains of progenitor-like cells and functional complexity of a stem cell niche in the neonatal rat spinal cord.
    Russo RE
    Stem cells (Dayton, Ohio) 30.9 (2012 Sep): 2020-31.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    In vitro effects of two silicate-based materials, Biodentine and BioRoot RCS, on dental pulp stem cells in models of reactionary and reparative dentinogenesis.
    Fournier BPJ
    PloS one 13.1 (2018): e0190014.

    All References

    Early expression of a novel radial glia antigen in the chick embryo.
    de la Rosa EJ
    Glia 15.4 (1995 Dec): 389-400.

    Vimentin isoform expression in the human retina characterized with the monoclonal antibody 3CB2.
    Prada C
    Journal of neuroscience research 86.8 (2008 Jun): 1871-83.

    Temporal progressive antigen expression in radial glia after contusive spinal cord injury in adult rats.
    Norimatsu H
    Glia 42.2 (2003 Apr 15): 172-83.

    GSK-3beta inhibition/beta-catenin stabilization in ventral midbrain precursors increases differentiation into dopamine neurons.
    Arenas E
    Journal of cell science 117.Pt 24 (2004 Nov 15): 5731-7.

    Wnt2b inhibits differentiation of retinal progenitor cells in the absence of Notch activity by downregulating the expression of proneural genes.
    Nakagawa S
    Development (Cambridge, England) 132.12 (2005 Jun): 2759-70.

    Stage and region dependent expression of a radial glial marker in commissural fibers in kindled mice.
    Itano T
    Epilepsy research 67.1-2 (2005 Oct-Nov): 61-72.

    Human embryonic stem cell derived astrocytes mediate non-cell-autonomous neuroprotection through endogenous and drug-induced mechanisms.
    Chandran S
    Cell death and differentiation 19.5 (2012 May): 779-87.

    Spatial domains of progenitor-like cells and functional complexity of a stem cell niche in the neonatal rat spinal cord.
    Russo RE
    Stem cells (Dayton, Ohio) 30.9 (2012 Sep): 2020-31.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    In vitro effects of two silicate-based materials, Biodentine and BioRoot RCS, on dental pulp stem cells in models of reactionary and reparative dentinogenesis.
    Fournier BPJ
    PloS one 13.1 (2018): e0190014.

    Matrix metalloproteinases are required for retinal ganglion cell axon guidance at select decision points.
    McFarlane S
    Development (Cambridge, England) 132.15 (2005 Aug): 3371-9.

    Niche-independent symmetrical self-renewal of a mammalian tissue stem cell.
    Smith A
    PLoS biology 3.9 (2005 Sep): e283.

    Identification of two novel glial-restricted cell populations in the embryonic telencephalon arising from unique origins.
    Mayer-Pröschel M
    BMC developmental biology 7. (2007 Apr 17): 33.

    Stable expression of intracellular Notch suppresses v-Src-induced transformation in avian neural cells.
    Calothy G
    Oncogene 26.23 (2007 May 17): 3338-51.

    CD133 (Prominin) negative human neural stem cells are clonogenic and tripotent.
    Smith A
    PloS one 4.5 (2009): e5498.

    Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens.
    Dirks P
    Cell stem cell 4.6 (2009 Jun 5): 568-80.

    Developmental regulation of human embryonic stem cell-derived neurons by calcium entry via transient receptor potential channels.
    Zhang SC
    Stem cells (Dayton, Ohio) 27.12 (2009 Dec): 2906-16.

    Real time imaging of human progenitor neurogenesis.
    Svendsen CN
    PloS one 5.10 (2010 Oct 7): e13187.

    Characterization of the antigenic phenotype of αB-crystallin-expressing peripapillary glial cells in the developing chick retina.
    Seo JH
    Anatomy and cell biology 44.1 (2011 Mar): 35-40.

    Interplay between FGF2 and BMP controls the self-renewal, dormancy and differentiation of rat neural stem cells.
    Smith A
    Journal of cell science 124.Pt 11 (2011 Jun 1): 1867-77.

    Glucose transporter 1 and monocarboxylate transporters 1, 2, and 4 localization within the glial cells of shark blood-brain-barriers.
    Nualart F
    PloS one 7.2 (2012): e32409.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    Effects of High-Temperature-Pressure Polymerized Resin-Infiltrated Ceramic Networks on Oral Stem Cells.
    Fournier BP
    PloS one 11.5 (2016): e0155450.

    A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome.
    Engle EC
    Nature communications 8. (2017 Jul 6): 16077.

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