3CB2
Catalog Fields
Antigen: Vimentin (radial glial and neuronal stem cells)
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: De La Rosa, E.J.
Isotype: MIgM
Depositors Institution: Centro de Investigaciones Biológicas CSIC, Madrid
Host Species: mouse
Depositors Notes: 3CB2 is a cell marker from neural stem cells and radial glia. 3CB2 also recognizes three isoforms of vimentin in gliomas.
Positive Tested Species Reactivity: Chameleon, Chick, Human, Mouse, Quail, Rat
Gene: VIM
Antigen Molecular Weight: 53.1 kDa
Clonality: Monoclonal
Immunogen: E8 optic tectum, fractionated
Immunogen Sequence: Full length sequence
Myeloma Strain: Ag8.653
Additional Information: RRID:AB_531888
Uniprot ID: P09654
Entrez Gene ID: 420519
Antibody Registry ID: AB_531888
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3CB2 was deposited to the DSHB by De La Rosa, E.J. (DSHB Hybridoma Product 3CB2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
24 References
Initial Publication
Early expression of a novel radial glia antigen in the chick embryo.
de la Rosa EJ
Glia 15.4 (1995 Dec): 389-400.