3CB2
Catalog Fields
Clone ID/Product Name: 3CB2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: VIM
Ab Isotype: MIgM
Gene Name:
Entrez Gene ID: 420519
Clonality: Monoclonal
Immunogen: E8 optic tectum, fractionated
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: Ag8.653
Epitope Mapped: No
Antigen Name: Vimentin (radial glial and neuronal stem cells)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/13/1996
Antigen Molecular Weight: 53.1 kDa
Depositor: De La Rosa, E.J.
Antigen Sequence:
Depositor Institution: Centro de Investigaciones Biologicas CSIC, Madrid
Antigen Species: Chick
Depositor Notes: 3CB2 is a cell marker from neural stem cells and radial glia. 3CB2 also recognizes three isoforms of vimentin in gliomas.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chameleon, Chick, Human, Mouse, Quail, Rat, Xenopus
Additional Information: RRID:AB_531888
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3CB2 was deposited to the DSHB by De La Rosa, E.J. (DSHB Hybridoma Product 3CB2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
25 References
Initial Publication
Early expression of a novel radial glia antigen in the chick embryo.
de la Rosa EJ
Glia 15.4 (1995 Dec): 389-400.