Antigen: Vimentin (radial glial and neuronal stem cells)
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: De La Rosa, E.J.
Host Species: mouse
Depositors Institution: Centro de Investigaciones BiolÃ³gicas CSIC, Madrid
Positive Tested Species Reactivity: Chameleon, Chick, Human, Mouse, Quail, Rat
Depositors Notes: 3CB2 is a cell marker from neural stem cells and radial glia. 3CB2 also recognizes three isoforms of vimentin in gliomas.
Predicted Species Reactivity:
Immunogen: E8 optic tectum, fractionated
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Ag8.653
Epitope Location or Sequence:
Uniprot ID: P09654
Immunogen Sequence: Full length sequence
Entrez Gene ID: 420519
Antibody Registry ID: AB_531888
Additional Information: RRID:AB_531888
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3CB2 was deposited to the DSHB by De La Rosa, E.J. (DSHB Hybridoma Product 3CB2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.