G3G4 (anti-BrdU)

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$40.00
SKU: G3G4 (anti-BrdU)
View product citations for antibody G3G4 (anti-BrdU) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: 5-bromo-2'-deoxyuridine BrdU
Hybridoma Cells Available: Yes
Antigen Species: Not applicable
Depositor: Kaufman, S.J.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Illinois
Positive Tested Species Reactivity: All species labeled with BrdU
Depositors Notes: This antibody detects BrdU incorporated into cell nuclei during S-phase. 2N HCL treatment of nuclei increases antibody access to incorporated BrdU within the DNA (see Foster et al., 1987 for details).
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: BrdU
Immunogen: 5-bromo-2'-deoxyuridine-BSA
Alternate Gene Names:
Alternate Antibody Name: G3G4(AntiBrdUrd), Bromodeoxyuridine
Clonality: Monoclonal
Alternate Antigen Name: Proliferation Marker
Epitope Mapped:
Myeloma Strain: Sp 2/0 Ag 14
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2314035 
Additional Information: IHC paper: Acta zoologica doi: 10.1111/azo.12114
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
G3G4 (anti-BrdU) was deposited to the DSHB by Kaufman, S.J. (DSHB Hybridoma Product G3G4 (anti-BrdU))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

36 References

  • Initial Publication
  • IF References
  • IHC References
  • FFPE References
  • FACS References
  • All References
  • Initial Publication
    IF References

    Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
    Jay P
    The Journal of cell biology 192.5 (2011 Mar 7): 767-80.

    Generation of GABAergic and dopaminergic interneurons from endogenous embryonic olfactory bulb precursor cells.
    Vicario-Abejón C
    Development (Cambridge, England) 133.21 (2006 Nov): 4367-79.

    Modulation of the PI 3-kinase-Akt signalling pathway by IGF-I and PTEN regulates the differentiation of neural stem/precursor cells.
    de Pablo F
    Journal of cell science 119.Pt 13 (2006 Jul 1): 2739-48.

    Absence of hematopoiesis from transplanted olfactory bulb neural stem cells.
    Vicario-Abejón C
    The European journal of neuroscience 19.3 (2004 Feb): 505-12.

    Bcl-2 expression identifies an early stage of myogenesis and promotes clonal expansion of muscle cells.
    Miller JB
    The Journal of cell biology 142.2 (1998 Jul 27): 537-44.

    Early neural cell death is an extensive, dynamic process in the embryonic chick and mouse retina.
    de la Rosa EJ
    TheScientificWorldJournal 2013. (2013): 627240.

    Defects in the outer limiting membrane are associated with rosette development in the Nrl-/- retina.
    Naash MI
    PloS one 7.3 (2012): e32484.

    Calcium influx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch.
    Tatsumi R
    American journal of physiology. Cell physiology 302.12 (2012 Jun 15): C1741-50.

    A2E accumulation influences retinal microglial activation and complement regulation.
    Wong WT
    Neurobiology of aging 34.3 (2013 Mar): 943-60.

    Rab11, a vesicular trafficking protein, affects endoreplication through Ras-mediated pathway in Drosophila melanogaster.
    Roy JK
    Cell and tissue research 367.2 (2017 Feb): 269-282.

    Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema.
    Alibardi L
    Acta histochemica 119.3 (2017 Apr): 211-219.

    MicroRNA-107 Targets IKBKG and Sensitizes A549 Cells to Parthenolide.
    Park JK
    Anticancer research 38.11 (2018 Nov): 6309-6316.

    Discs large 1 controls daughter-cell polarity after cytokinesis in vertebrate morphogenesis.
    Fraser SE
    Proceedings of the National Academy of Sciences of the United States of America 115.46 (2018 Nov 13): E10859-E10868.

    GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice.
    Wang C
    BMC biology 17.1 (2019 Mar 12): 23.

    IHC References

    The Retinal Homeobox (Rx) gene is necessary for retinal regeneration.
    El-Hodiri HM
    Developmental biology 353.1 (2011 May 1): 10-8.

    Epithelial-mesenchymal transition and proliferation of retinal pigment epithelial cells initiated upon loss of cell-cell contact.
    Kaplan HJ
    Investigative ophthalmology and visual science 51.5 (2010 May): 2755-63.

    Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells.
    Gan L
    Gene expression patterns : GEP 7.1-2 (2007 Jan): 57-65.

    Generation of GABAergic and dopaminergic interneurons from endogenous embryonic olfactory bulb precursor cells.
    Vicario-Abejón C
    Development (Cambridge, England) 133.21 (2006 Nov): 4367-79.

    Spatial and temporal patterns of proliferation and differentiation in the developing turtle eye.
    Martín-Partido G
    Brain research 1103.1 (2006 Aug 4): 32-48.

    Docosahexaenoic acid promotes photoreceptor differentiation without altering Crx expression.
    Politi LE
    Investigative ophthalmology and visual science 47.7 (2006 Jul): 3017-27.

    Modulation of the PI 3-kinase-Akt signalling pathway by IGF-I and PTEN regulates the differentiation of neural stem/precursor cells.
    de Pablo F
    Journal of cell science 119.Pt 13 (2006 Jul 1): 2739-48.

    Locally born olfactory bulb stem cells proliferate in response to insulin-related factors and require endogenous insulin-like growth factor-I for differentiation into neurons and glia.
    de Pablo F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.3 (2003 Feb 1): 895-906.

    In vitro and in vivo expression of alpha 7 integrin and desmin define the primary and secondary myogenic lineages.
    Kaufman SJ
    Developmental biology 156.1 (1993 Mar): 209-29.

    The Retinal Homeobox (Rx) gene is necessary for retinal regeneration.
    El-Hodiri HM
    Developmental biology 353.1 (2011 May 1): 10-8.

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    Ric-8a, a guanine nucleotide exchange factor for heterotrimeric G proteins, regulates bergmann glia-basement membrane adhesion during cerebellar foliation.
    Huang Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.43 (2012 Oct 24): 14979-93.

    Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes.
    Miyata S
    Cell biochemistry and function 30.5 (2012 Jul): 400-5.

    Ganglioside GD3 is required for neurogenesis and long-term maintenance of neural stem cells in the postnatal mouse brain.
    Yu RK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.41 (2014 Oct 8): 13790-800.

    Pharmacological administration of the isoflavone daidzein enhances cell proliferation and reduces high fat diet-induced apoptosis and gliosis in the rat hippocampus.
    Suárez J
    PloS one 8.5 (2013): e64750.

    Temporally tuned neuronal differentiation supports the functional remodeling of a neuronal network in Drosophila.
    Allan DW
    Proceedings of the National Academy of Sciences of the United States of America 109.13 (2012 Mar 27): E748-56.

    Role of retinal pigment epithelial cell β-catenin signaling in experimental proliferative vitreoretinopathy.
    Tamiya S
    The American journal of pathology 184.5 (2014 May): 1419-28.

    [Pituitary reserve of growth hormone in adolescent patients with cerebral palsy and mental retardation].
    Kurashige T
    No to hattatsu = Brain and development 21.3 (1989 May): 239-44.

    Regulation of ADSC Osteoinductive Potential Using Notch Pathway Inhibition and Gene Rescue: A Potential On/Off Switch for Clinical Applications in Bone Formation and Reconstructive Efforts.
    Neumeister MW
    Plastic and reconstructive surgery 138.4 (2016 Oct): 642e-52e.



    . (): .

    PAK4 suppresses RELB to prevent senescence-like growth arrest in breast cancer.
    Strömblad S
    Nature communications 10.1 (2019 Aug 9): 3589.

    Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema.
    Alibardi L
    Acta histochemica 119.3 (2017 Apr): 211-219.

    FFPE References
    FACS References
    All References

    The Retinal Homeobox (Rx) gene is necessary for retinal regeneration.
    El-Hodiri HM
    Developmental biology 353.1 (2011 May 1): 10-8.

    Epithelial-mesenchymal transition and proliferation of retinal pigment epithelial cells initiated upon loss of cell-cell contact.
    Kaplan HJ
    Investigative ophthalmology and visual science 51.5 (2010 May): 2755-63.

    Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells.
    Gan L
    Gene expression patterns : GEP 7.1-2 (2007 Jan): 57-65.

    Generation of GABAergic and dopaminergic interneurons from endogenous embryonic olfactory bulb precursor cells.
    Vicario-Abejón C
    Development (Cambridge, England) 133.21 (2006 Nov): 4367-79.

    Spatial and temporal patterns of proliferation and differentiation in the developing turtle eye.
    Martín-Partido G
    Brain research 1103.1 (2006 Aug 4): 32-48.

    Docosahexaenoic acid promotes photoreceptor differentiation without altering Crx expression.
    Politi LE
    Investigative ophthalmology and visual science 47.7 (2006 Jul): 3017-27.

    Modulation of the PI 3-kinase-Akt signalling pathway by IGF-I and PTEN regulates the differentiation of neural stem/precursor cells.
    de Pablo F
    Journal of cell science 119.Pt 13 (2006 Jul 1): 2739-48.

    Locally born olfactory bulb stem cells proliferate in response to insulin-related factors and require endogenous insulin-like growth factor-I for differentiation into neurons and glia.
    de Pablo F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.3 (2003 Feb 1): 895-906.

    In vitro and in vivo expression of alpha 7 integrin and desmin define the primary and secondary myogenic lineages.
    Kaufman SJ
    Developmental biology 156.1 (1993 Mar): 209-29.

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    Ric-8a, a guanine nucleotide exchange factor for heterotrimeric G proteins, regulates bergmann glia-basement membrane adhesion during cerebellar foliation.
    Huang Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.43 (2012 Oct 24): 14979-93.

    Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes.
    Miyata S
    Cell biochemistry and function 30.5 (2012 Jul): 400-5.

    Ganglioside GD3 is required for neurogenesis and long-term maintenance of neural stem cells in the postnatal mouse brain.
    Yu RK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.41 (2014 Oct 8): 13790-800.

    Pharmacological administration of the isoflavone daidzein enhances cell proliferation and reduces high fat diet-induced apoptosis and gliosis in the rat hippocampus.
    Suárez J
    PloS one 8.5 (2013): e64750.

    Temporally tuned neuronal differentiation supports the functional remodeling of a neuronal network in Drosophila.
    Allan DW
    Proceedings of the National Academy of Sciences of the United States of America 109.13 (2012 Mar 27): E748-56.

    Role of retinal pigment epithelial cell β-catenin signaling in experimental proliferative vitreoretinopathy.
    Tamiya S
    The American journal of pathology 184.5 (2014 May): 1419-28.

    [Pituitary reserve of growth hormone in adolescent patients with cerebral palsy and mental retardation].
    Kurashige T
    No to hattatsu = Brain and development 21.3 (1989 May): 239-44.

    Regulation of ADSC Osteoinductive Potential Using Notch Pathway Inhibition and Gene Rescue: A Potential On/Off Switch for Clinical Applications in Bone Formation and Reconstructive Efforts.
    Neumeister MW
    Plastic and reconstructive surgery 138.4 (2016 Oct): 642e-52e.



    . (): .

    PAK4 suppresses RELB to prevent senescence-like growth arrest in breast cancer.
    Strömblad S
    Nature communications 10.1 (2019 Aug 9): 3589.

    Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema.
    Alibardi L
    Acta histochemica 119.3 (2017 Apr): 211-219.

    Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
    Jay P
    The Journal of cell biology 192.5 (2011 Mar 7): 767-80.

    Absence of hematopoiesis from transplanted olfactory bulb neural stem cells.
    Vicario-Abejón C
    The European journal of neuroscience 19.3 (2004 Feb): 505-12.

    Bcl-2 expression identifies an early stage of myogenesis and promotes clonal expansion of muscle cells.
    Miller JB
    The Journal of cell biology 142.2 (1998 Jul 27): 537-44.

    Early neural cell death is an extensive, dynamic process in the embryonic chick and mouse retina.
    de la Rosa EJ
    TheScientificWorldJournal 2013. (2013): 627240.

    Defects in the outer limiting membrane are associated with rosette development in the Nrl-/- retina.
    Naash MI
    PloS one 7.3 (2012): e32484.

    Calcium influx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch.
    Tatsumi R
    American journal of physiology. Cell physiology 302.12 (2012 Jun 15): C1741-50.

    A2E accumulation influences retinal microglial activation and complement regulation.
    Wong WT
    Neurobiology of aging 34.3 (2013 Mar): 943-60.

    Rab11, a vesicular trafficking protein, affects endoreplication through Ras-mediated pathway in Drosophila melanogaster.
    Roy JK
    Cell and tissue research 367.2 (2017 Feb): 269-282.

    MicroRNA-107 Targets IKBKG and Sensitizes A549 Cells to Parthenolide.
    Park JK
    Anticancer research 38.11 (2018 Nov): 6309-6316.

    Discs large 1 controls daughter-cell polarity after cytokinesis in vertebrate morphogenesis.
    Fraser SE
    Proceedings of the National Academy of Sciences of the United States of America 115.46 (2018 Nov 13): E10859-E10868.

    GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice.
    Wang C
    BMC biology 17.1 (2019 Mar 12): 23.

    Mesodermal expression of integrin α5β1 regulates neural crest development and cardiovascular morphogenesis.
    Astrof S
    Developmental biology 395.2 (2014 Nov 15): 232-44.

    Mitochondrial Fragmentation Due to Inhibition of Fusion Increases Cyclin B through Mitochondrial Superoxide Radicals.
    Gupte TM
    PloS one 10.5 (2015): e0126829.

    A laminin substrate promotes myogenesis in rat skeletal muscle cultures: analysis of replication and development using antidesmin and anti-BrdUrd monoclonal antibodies.
    Kaufman SJ
    Developmental biology 122.1 (1987 Jul): 11-20.

    Autophagy inhibition re-sensitizes pulse stimulation-selected paclitaxel-resistant triple negative breast cancer cells to chemotherapy-induced apoptosis.
    Guan JL
    Breast cancer research and treatment 149.3 (2015 Feb): 619-29.

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