RC2
Catalog Fields
Clone ID/Product Name: RC2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Nes
Ab Isotype: MIgM, lambda light chain
Gene Name:
Entrez Gene ID: 18008
Clonality: Monoclonal
Immunogen: Fetal brain cell lysate (E14–E17)
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Nestin/radial glial marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/2/1996
Antigen Molecular Weight: 295 kDa Apparent
Depositor: Yamamoto, M.
Antigen Sequence:
Depositor Institution: University of Tsukuba
Antigen Species: Rat
Depositor Notes: This antibody recognizes the intermediate filament nestin and is a marker for pluripotent neural progenitor cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RC2 was deposited to the DSHB by Yamamoto, M. (DSHB Hybridoma Product RC2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
35 References
Initial Publication
Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker.
Caviness VS Jr
Brain research. Developmental brain research 44.1 (1988 Nov 1): 95-108.