RC2

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$40.00
SKU: RC2
View product citations for antibody RC2 on CiteAb

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Available: 185

DSHB Data Sheet

Catalog Fields

Antigen: Nestin/radial glial marker
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Yamamoto, M.
Isotype: MIgM, lambda light chain
Depositors Institution: University of Tsukuba
Host Species: mouse
Depositors Notes: This antibody recognizes the intermediate filament nestin and is a marker for pluripotent neural progenitor cells.
Positive Tested Species Reactivity: Mouse
Gene: Nes
Antigen Molecular Weight: 295 kDa Apparent
Clonality: Monoclonal
Immunogen: Fetal brain cell lysate (E14–E17)
Epitope Mapped: No
Myeloma Strain: NS-1
Uniprot ID: Q6P5H2 
Entrez Gene ID: 18008 
Antibody Registry ID: AB_531887 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RC2 was deposited to the DSHB by Yamamoto, M. (DSHB Hybridoma Product RC2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

34 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Multiple telencephalic and extratelencephalic embryonic domains contribute neurons to the medial extended amygdala.
    Medina L
    The Journal of comparative neurology 519.8 (2011 Jun 1): 1505-25.

    Shp2-dependent ERK signaling is essential for induction of Bergmann glia and foliation of the cerebellum.
    Li JY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.3 (2014 Jan 15): 922-31.

    MiR-30e and miR-181d control radial glia cell proliferation via HtrA1 modulation.
    Muzio L
    Cell death & disease 3. (2012 Aug 2): e360.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    F3/Contactin acts as a modulator of neurogenesis during cerebral cortex development.
    Gennarini G
    Developmental biology 365.1 (2012 May 1): 133-51.

    Comparison of fluid transport systems in lymphatics and veins.
    Roddie IC
    Lymphology 20.4 (1987 Dec): 224-9.

    Breaches of the pial basement membrane and disappearance of the glia limitans during development underlie the cortical lamination defect in the mouse model of muscle-eye-brain disease.
    Qi Y
    The Journal of comparative neurology 501.1 (2007 Mar 1): 168-83.

    Development of the mouse amygdala as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation.
    Yuasa S
    The Journal of comparative neurology 513.1 (2009 Mar 1): 113-28.

    Reciprocal induction of simple organogenesis by mouse kidney progenitor cells in three-dimensional co-culture.
    Barnes JL
    The American journal of pathology 180.2 (2012 Feb): 819-30.

    Disease-associated missense mutations in bestrophin-1 affect cellular trafficking and anion conductance.
    Strauss O
    Journal of cell science 124.Pt 17 (2011 Sep 1): 2988-96.

    Intrinsic and extrinsic connections of Tet3 dioxygenase with CXXC zinc finger modules.
    Spada F
    PloS one 8.5 (2013): e62755.

    Generation of regionally specified neurons in expanded glial cultures derived from the mouse and human lateral ganglionic eminence.
    Campbell K
    Molecular and cellular neurosciences 17.5 (2001 May): 811-20.

    Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
    DiCicco-Bloom E
    Proceedings of the National Academy of Sciences of the United States of America 98.8 (2001 Apr 10): 4758-63.

    Characterization of CNS precursor subtypes and radial glia.
    Götz M
    Developmental biology 229.1 (2001 Jan 1): 15-30.

    A subset of fibroblast growth factors (Fgfs) promote survival, but Fgf-8b specifically promotes astroglial differentiation of rat cortical precursor cells.
    Dickson C
    Molecular and cellular neurosciences 14.6 (1999 Dec): 468-85.

    Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor cell populations.
    Magnuson DS
    Experimental cell research 252.1 (1999 Oct 10): 75-95.

    Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye.
    Baker NE
    Current biology : CB 11.5 (2001 Mar 6): 330-8.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    Self-renewal and differentiation of reactive astrocyte-derived neural stem/progenitor cells isolated from the cortical peri-infarct area after stroke.
    Spees JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.23 (2012 Jun 6): 7926-40.

    Dpy19l1, a multi-transmembrane protein, regulates the radial migration of glutamatergic neurons in the developing cerebral cortex.
    Tamamaki N
    Development (Cambridge, England) 138.22 (2011 Nov): 4979-90.

    C3G regulates cortical neuron migration, preplate splitting and radial glial cell attachment.
    Thomas T
    Development (Cambridge, England) 135.12 (2008 Jun): 2139-49.

    The endocannabinoid system promotes astroglial differentiation by acting on neural progenitor cells.
    Galve-Roperh I
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.5 (2006 Feb 1): 1551-61.

    A unique subpopulation of Tbr1-expressing deep layer neurons in the developing cerebral cortex.
    Donoghue MJ
    Molecular and cellular neurosciences 30.4 (2005 Dec): 538-51.

    Integrin-linked kinase deletion from mouse cortex results in cortical lamination defects resembling cobblestone lissencephaly.
    Reichardt LF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.30 (2005 Jul 27): 7022-31.

    Generation of regionally specified neurons in expanded glial cultures derived from the mouse and human lateral ganglionic eminence.
    Campbell K
    Molecular and cellular neurosciences 17.5 (2001 May): 811-20.

    Retinoids are produced by glia in the lateral ganglionic eminence and regulate striatal neuron differentiation.
    Campbell K
    Development (Cambridge, England) 126.6 (1999 Mar): 1317-26.

    The first retinal axon growth in the mouse optic chiasm: axon patterning and the cellular environment.
    Mason CA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.10 (1995 Oct): 6389-402.

    Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker.
    Caviness VS Jr
    Brain research. Developmental brain research 44.1 (1988 Nov 1): 95-108.

    FFPE References
    All References

    Multiple telencephalic and extratelencephalic embryonic domains contribute neurons to the medial extended amygdala.
    Medina L
    The Journal of comparative neurology 519.8 (2011 Jun 1): 1505-25.

    Shp2-dependent ERK signaling is essential for induction of Bergmann glia and foliation of the cerebellum.
    Li JY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.3 (2014 Jan 15): 922-31.

    MiR-30e and miR-181d control radial glia cell proliferation via HtrA1 modulation.
    Muzio L
    Cell death & disease 3. (2012 Aug 2): e360.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    F3/Contactin acts as a modulator of neurogenesis during cerebral cortex development.
    Gennarini G
    Developmental biology 365.1 (2012 May 1): 133-51.

    Comparison of fluid transport systems in lymphatics and veins.
    Roddie IC
    Lymphology 20.4 (1987 Dec): 224-9.

    Breaches of the pial basement membrane and disappearance of the glia limitans during development underlie the cortical lamination defect in the mouse model of muscle-eye-brain disease.
    Qi Y
    The Journal of comparative neurology 501.1 (2007 Mar 1): 168-83.

    Development of the mouse amygdala as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation.
    Yuasa S
    The Journal of comparative neurology 513.1 (2009 Mar 1): 113-28.

    Reciprocal induction of simple organogenesis by mouse kidney progenitor cells in three-dimensional co-culture.
    Barnes JL
    The American journal of pathology 180.2 (2012 Feb): 819-30.

    Disease-associated missense mutations in bestrophin-1 affect cellular trafficking and anion conductance.
    Strauss O
    Journal of cell science 124.Pt 17 (2011 Sep 1): 2988-96.

    Intrinsic and extrinsic connections of Tet3 dioxygenase with CXXC zinc finger modules.
    Spada F
    PloS one 8.5 (2013): e62755.

    Generation of regionally specified neurons in expanded glial cultures derived from the mouse and human lateral ganglionic eminence.
    Campbell K
    Molecular and cellular neurosciences 17.5 (2001 May): 811-20.

    Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
    DiCicco-Bloom E
    Proceedings of the National Academy of Sciences of the United States of America 98.8 (2001 Apr 10): 4758-63.

    Characterization of CNS precursor subtypes and radial glia.
    Götz M
    Developmental biology 229.1 (2001 Jan 1): 15-30.

    A subset of fibroblast growth factors (Fgfs) promote survival, but Fgf-8b specifically promotes astroglial differentiation of rat cortical precursor cells.
    Dickson C
    Molecular and cellular neurosciences 14.6 (1999 Dec): 468-85.

    Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor cell populations.
    Magnuson DS
    Experimental cell research 252.1 (1999 Oct 10): 75-95.

    Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye.
    Baker NE
    Current biology : CB 11.5 (2001 Mar 6): 330-8.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    Self-renewal and differentiation of reactive astrocyte-derived neural stem/progenitor cells isolated from the cortical peri-infarct area after stroke.
    Spees JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.23 (2012 Jun 6): 7926-40.

    Dpy19l1, a multi-transmembrane protein, regulates the radial migration of glutamatergic neurons in the developing cerebral cortex.
    Tamamaki N
    Development (Cambridge, England) 138.22 (2011 Nov): 4979-90.

    C3G regulates cortical neuron migration, preplate splitting and radial glial cell attachment.
    Thomas T
    Development (Cambridge, England) 135.12 (2008 Jun): 2139-49.

    The endocannabinoid system promotes astroglial differentiation by acting on neural progenitor cells.
    Galve-Roperh I
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.5 (2006 Feb 1): 1551-61.

    A unique subpopulation of Tbr1-expressing deep layer neurons in the developing cerebral cortex.
    Donoghue MJ
    Molecular and cellular neurosciences 30.4 (2005 Dec): 538-51.

    Integrin-linked kinase deletion from mouse cortex results in cortical lamination defects resembling cobblestone lissencephaly.
    Reichardt LF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.30 (2005 Jul 27): 7022-31.

    Retinoids are produced by glia in the lateral ganglionic eminence and regulate striatal neuron differentiation.
    Campbell K
    Development (Cambridge, England) 126.6 (1999 Mar): 1317-26.

    The first retinal axon growth in the mouse optic chiasm: axon patterning and the cellular environment.
    Mason CA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.10 (1995 Oct): 6389-402.

    Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker.
    Caviness VS Jr
    Brain research. Developmental brain research 44.1 (1988 Nov 1): 95-108.

    Conditional knockout of protein O-mannosyltransferase 2 reveals tissue-specific roles of O-mannosyl glycosylation in brain development.
    Zhang P
    The Journal of comparative neurology 519.7 (2011 May 1): 1320-37.

    Xenogeneic transfer of adult quail (Coturnix coturnix) spermatogonial stem cells to embryonic chicken (Gallus gallus) hosts: a model for avian conservation.
    Jensen T
    Biology of reproduction 88.5 (2013 May): 129.

    Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage.
    Wolfe JH
    Stem cells translational medicine 2.6 (2013 Jun): 444-54.

    Commissural axonal corridors instruct neuronal migration in the mouse spinal cord.
    Wilson SI
    Nature communications 6. (2015 May 11): 7028.

    Isoform-specific localization of Nogo protein in the optic pathway of mouse embryos.
    Chan SO
    The Journal of comparative neurology 524.11 (2016 Aug 1): 2322-34.

    Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis.
    Lopez-Toledano MA
    International journal of Alzheimer's disease 2011. (2011): 929042.

    The homeodomain protein vax1 is required for axon guidance and major tract formation in the developing forebrain.
    Lemke G
    Genes & development 13.23 (1999 Dec 1): 3092-105.

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