5C6

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SKU: 5C6-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 5C6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528174 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: human fetal membranes
Clone:
Immunogen Sequence:
Myeloma Strain: X63Ag8.653
Epitope Mapped: No
Antigen Name: collagen type VI
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/8/1987
Antigen Molecular Weight:
Depositor: Engvall, E.S.
Antigen Sequence:
Depositor Institution: La Jolla Cancer Research Foundation
Antigen Species: human
Depositor Notes: Antibody does not work in immunoblotting. Stains type VI collagen in immunofluorescence. Specific for central, collagenous domain of type VI collagen.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Rabbit
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5C6 was deposited to the DSHB by Engvall, E.S. (DSHB Hybridoma Product 5C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication

    Type VI collagen. Studies on its localization, structure, and biosynthetic form with monoclonal antibodies.
    Engvall E
    The Journal of biological chemistry 259.6 (1984 Mar 25): 3955-61.

    IHC References
    All References

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