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SKU: 5C6-s
View product citations for antibody 5C6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: collagen type VI
Hybridoma Cells Available: No
Antigen Species: human
Depositor: Engvall, E.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: La Jolla Cancer Research Foundation,
Positive Tested Species Reactivity: Human, Rabbit
Depositors Notes: Antibody does not work in immunoblotting. Stains type VI collagen in immunofluorescence. Specific for central, collagenous domain of type VI collagen.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: human fetal membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5C6 was deposited to the DSHB by Engvall, E.S. (DSHB Hybridoma Product 5C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication

    Type VI collagen. Studies on its localization, structure, and biosynthetic form with monoclonal antibodies.
    Engvall E
    The Journal of biological chemistry 259.6 (1984 Mar 25): 3955-61.

    IHC References
    All References

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