M-38
Catalog Fields
Antigen: collagen(pro-) type I
Hybridoma Cells Available:
Antigen Species: Human
Depositor: McDonald, J.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Mayo Clinic Scottsdale
Positive Tested Species Reactivity: Bovine, Chicken, Guinea Pig, Human
Depositors Notes: The epitope recognized by this antibody is destroyed by formalin. Frozen or acid alcohol fixed tissue is recommended. This antibody does not recognize mouse, rat or hamster procollagen type I.
Antigen Molecular Weight: 140-150 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: COL1A1
Immunogen: Ascorbate-supplemented human IMR-90 cells (lung fibroblast)
Alternate Gene Names: OI4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP-2/0-Ag 14
Epitope Location or Sequence:
Uniprot ID: P02452
Immunogen Sequence: Carboxyterminal propeptide type I collagen
Entrez Gene ID: 1277
Additional Characterization:
Antibody Registry ID: AB_2245128
Additional Information: RRID:AB_2245128
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M-38 was deposited to the DSHB by McDonald, J.A. (DSHB Hybridoma Product M-38)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
23 References
Initial Publication
A monoclonal antibody to the carboxyterminal domain of procollagen type I visualizes collagen-synthesizing fibroblasts. Detection of an altered fibroblast phenotype in lungs of patients with pulmonary fibrosis.
Kuhn C 3rd
The Journal of clinical investigation 78.5 (1986 Nov): 1237-44.