M-38
Catalog Fields
Antigen: collagen(pro-) type I
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: McDonald, J.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Mayo Clinic Scottsdale
Positive Tested Species Reactivity: Bovine, Chicken, Guinea Pig, Human
Depositors Notes: The epitope recognized by this antibody is destroyed by formalin. Frozen or acid alcohol fixed tissue is recommended. This antibody does not recognize mouse, rat or hamster procollagen type I.
Predicted Species Reactivity:
Gene: COL1A1
Immunogen: Ascorbate-supplemented human IMR-90 cells (lung fibroblast)
Alternate Gene Names: OI4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP-2/0-Ag 14
Epitope Location or Sequence:
Uniprot ID: P02452
Immunogen Sequence: Carboxyterminal propeptide type I collagen
Entrez Gene ID: 1277
Antibody Registry ID: AB_2245128
Additional Information: RRID:AB_2245128
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M-38 was deposited to the DSHB by McDonald, J.A. (DSHB Hybridoma Product M-38)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
23 References
Initial Publication
A monoclonal antibody to the carboxyterminal domain of procollagen type I visualizes collagen-synthesizing fibroblasts. Detection of an altered fibroblast phenotype in lungs of patients with pulmonary fibrosis.
Kuhn C 3rd
The Journal of clinical investigation 78.5 (1986 Nov): 1237-44.