M-38

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$40.00
SKU: M-38
View product citations for antibody M-38 on CiteAb

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Available: 168

DSHB Data Sheet

Catalog Fields

Antigen: collagen(pro-) type I
Hybridoma Cells Available:
Antigen Species: Human
Depositor: McDonald, J.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Mayo Clinic Scottsdale
Positive Tested Species Reactivity: Bovine, Chicken, Guinea Pig, Human
Depositors Notes: The epitope recognized by this antibody is destroyed by formalin. Frozen or acid alcohol fixed tissue is recommended. This antibody does not recognize mouse, rat or hamster procollagen type I.
Antigen Molecular Weight: 140-150 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: COL1A1
Immunogen: Ascorbate-supplemented human IMR-90 cells (lung fibroblast)
Alternate Gene Names: OI4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP-2/0-Ag 14
Epitope Location or Sequence:
Uniprot ID: P02452 
Immunogen Sequence: Carboxyterminal propeptide type I collagen
Entrez Gene ID: 1277 
Additional Characterization:
Antibody Registry ID: AB_2245128 
Additional Information: RRID:AB_2245128
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M-38 was deposited to the DSHB by McDonald, J.A. (DSHB Hybridoma Product M-38)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

23 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    A monoclonal antibody to the carboxyterminal domain of procollagen type I visualizes collagen-synthesizing fibroblasts. Detection of an altered fibroblast phenotype in lungs of patients with pulmonary fibrosis.
    Kuhn C 3rd
    The Journal of clinical investigation 78.5 (1986 Nov): 1237-44.

    NaCl potentiates human fibrocyte differentiation.
    Gomer RH
    PloS one 7.9 (2012): e45674.

    Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development.
    Nusse R
    Development (Cambridge, England) 135.19 (2008 Oct): 3247-57.

    Type XXVII collagen at the transition of cartilage to bone during skeletogenesis.
    Pace JM
    Bone 41.4 (2007 Oct): 535-42.

    Molecular markers in patients with chronic wounds to guide surgical debridement.
    Tomic-Canic M
    Molecular medicine (Cambridge, Mass.) 13.1-2 (2007 Jan-Feb): 30-9.

    Increased arterial load alters aortic structural and functional properties during embryogenesis.
    Keller BB
    American journal of physiology. Heart and circulatory physiology 291.4 (2006 Oct): H1919-26.

    In vivo generation of cartilage from periosteum.
    Kuijer R
    Tissue engineering 11.3-4 (2005 Mar-Apr): 369-77.

    Improved cartilage integration and interfacial strength after enzymatic treatment in a cartilage transplantation model.
    van Osch GJ
    Arthritis research and therapy 6.5 (2004): R469-76.

    Functional tissue engineering of autologous tunica albuginea: a possible graft for Peyronie's disease surgery.
    Jonas U
    European urology 45.6 (2004 Jun): 781-6.

    Architectural transcription factor HMGI(Y) promotes tumor progression and mesenchymal transition of human epithelial cells.
    Li Y
    Molecular and cellular biology 21.2 (2001 Jan): 575-94.

    A new approach to demonstrate cellular activity in bone formation adjacent to implants.
    Albrektsson T
    Journal of biomedical materials research 51.2 (2000 Aug): 280-91.

    The teratogenic effects of valproic acid in human chondrogenesis in vitro.
    Hitt DC
    Teratology 49.3 (1994 Mar): 208-17.

    IP References

    D-periodic assemblies of type I procollagen.
    Chapman JA
    Journal of molecular biology 211.3 (1990 Feb 5): 581-94.

    ELISA References

    Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen.
    Risteli J
    Clinical chemistry 36.7 (1990 Jul): 1328-32.

    FFPE References
    FACS References

    The pathology of bone tissue during peri-implantitis.
    Miosge N
    Journal of dental research 94.2 (2015 Feb): 354-61.

    Epitope Map References

    D-periodic assemblies of type I procollagen.
    Chapman JA
    Journal of molecular biology 211.3 (1990 Feb 5): 581-94.

    All References

    A monoclonal antibody to the carboxyterminal domain of procollagen type I visualizes collagen-synthesizing fibroblasts. Detection of an altered fibroblast phenotype in lungs of patients with pulmonary fibrosis.
    Kuhn C 3rd
    The Journal of clinical investigation 78.5 (1986 Nov): 1237-44.

    NaCl potentiates human fibrocyte differentiation.
    Gomer RH
    PloS one 7.9 (2012): e45674.

    Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development.
    Nusse R
    Development (Cambridge, England) 135.19 (2008 Oct): 3247-57.

    Type XXVII collagen at the transition of cartilage to bone during skeletogenesis.
    Pace JM
    Bone 41.4 (2007 Oct): 535-42.

    Molecular markers in patients with chronic wounds to guide surgical debridement.
    Tomic-Canic M
    Molecular medicine (Cambridge, Mass.) 13.1-2 (2007 Jan-Feb): 30-9.

    Increased arterial load alters aortic structural and functional properties during embryogenesis.
    Keller BB
    American journal of physiology. Heart and circulatory physiology 291.4 (2006 Oct): H1919-26.

    In vivo generation of cartilage from periosteum.
    Kuijer R
    Tissue engineering 11.3-4 (2005 Mar-Apr): 369-77.

    Improved cartilage integration and interfacial strength after enzymatic treatment in a cartilage transplantation model.
    van Osch GJ
    Arthritis research and therapy 6.5 (2004): R469-76.

    Functional tissue engineering of autologous tunica albuginea: a possible graft for Peyronie's disease surgery.
    Jonas U
    European urology 45.6 (2004 Jun): 781-6.

    Architectural transcription factor HMGI(Y) promotes tumor progression and mesenchymal transition of human epithelial cells.
    Li Y
    Molecular and cellular biology 21.2 (2001 Jan): 575-94.

    A new approach to demonstrate cellular activity in bone formation adjacent to implants.
    Albrektsson T
    Journal of biomedical materials research 51.2 (2000 Aug): 280-91.

    The teratogenic effects of valproic acid in human chondrogenesis in vitro.
    Hitt DC
    Teratology 49.3 (1994 Mar): 208-17.

    A new method to investigate how mechanical loading of osteocytes controls osteoblasts.
    Mason DJ
    Frontiers in endocrinology 5. (2014): 208.

    Chondrocyte culture in three dimensional alginate sulfate hydrogels promotes proliferation while maintaining expression of chondrogenic markers.
    Zenobi-Wong M
    Tissue engineering. Part A 20.9-10 (2014 May): 1454-64.

    Valproate reduces collagen and osteonectin in cultured bone cells.
    Fuller HR
    Epilepsy research 106.3 (2013 Oct): 446-50.

    Valproate and bone loss: iTRAQ proteomics show that valproate reduces collagens and osteonectin in SMA cells.
    Morris GE
    Journal of proteome research 9.8 (2010 Aug 6): 4228-33.

    Wnt signaling in heart valve development and osteogenic gene induction.
    Yutzey KE
    Developmental biology 338.2 (2010 Feb 15): 127-35.

    Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis.
    Miosge N
    Cell stem cell 4.4 (2009 Apr 3): 324-35.

    Actin and type I collagen propeptide distribution in the developing chick cornea.
    Ralphs JR
    Investigative ophthalmology and visual science 50.4 (2009 Apr): 1653-8.

    The pathology of bone tissue during peri-implantitis.
    Miosge N
    Journal of dental research 94.2 (2015 Feb): 354-61.

    Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen.
    Risteli J
    Clinical chemistry 36.7 (1990 Jul): 1328-32.

    D-periodic assemblies of type I procollagen.
    Chapman JA
    Journal of molecular biology 211.3 (1990 Feb 5): 581-94.

    Molecular pathogenesis of chronic wounds: the role of beta-catenin and c-myc in the inhibition of epithelialization and wound healing.
    Tomic-Canic M
    The American journal of pathology 167.1 (2005 Jul): 59-69.

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