7A6

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$40.00
SKU: 7A6
View product citations for antibody 7A6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Nuclear factor of activated T-cells, cytoplasmic 1
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Crabtree, G.R.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Beckman Center, Stanford University
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: multiple isoforms from 39 to 101 kDa; Apparent: phosphorylated form (cytoplasmic) at 150, 120, and 80 kDa, and dephosphorylated form (nuclear) at 120, 80, and 68 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: NFATC1
Immunogen: Recombinant GST/NFATC1 (aa 1-654 of isoform A-alpha) fusion protein
Alternate Gene Names: NF-ATC, NFAT2, NFATc
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: aa 186-380
Uniprot ID: O95644 
Immunogen Sequence: partial protein
Entrez Gene ID: 4772 
Additional Characterization:
Antibody Registry ID: AB_2152505 
Additional Information:
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7A6 was deposited to the DSHB by Crabtree, G.R. (DSHB Hybridoma Product 7A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References
    WB References

    Nuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-Fos.
    Wagner EF
    The Journal of biological chemistry 279.25 (2004 Jun 18): 26475-80.

    Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction.
    Crabtree GR
    Genes & development 11.7 (1997 Apr 1): 824-34.

    Redundant expression but selective utilization of nuclear factor of activated T cells family members.
    Crabtree GR
    Journal of immunology (Baltimore, Md. : 1950) 159.6 (1997 Sep 15): 2735-40.

    Role of the NF-ATc transcription factor in morphogenesis of cardiac valves and septum.
    Mak TW
    Nature 392.6672 (1998 Mar 12): 182-6.

    NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity.
    Crabtree GR
    The Journal of biological chemistry 270.34 (1995 Aug 25): 19898-907.

    Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction.
    Crabtree GR
    Current biology : CB 8.10 (1998 May 7): 563-72.

    Different nuclear signals are activated by the B cell receptor during positive versus negative signaling.
    Goodnow CC
    Immunity 6.4 (1997 Apr): 419-28.

    Protein kinase A negatively modulates the nuclear accumulation of NF-ATc1 by priming for subsequent phosphorylation by glycogen synthase kinase-3.
    Gardner P
    The Journal of biological chemistry 277.50 (2002 Dec 13): 48664-76.

    Selective role of NFATc3 in positive selection of thymocytes.
    Crabtree GR
    Journal of immunology (Baltimore, Md. : 1950) 179.1 (2007 Jul 1): 103-10.

    Nuclear factor of activated T cells (NFAT) as a new component of the signal transduction pathway in glioma cells.
    Kaminska B
    Journal of neurochemistry 71.1 (1998 Jul): 134-41.

    IHC References
    IP References
    Epitope Map References
    Gel Supershift References

    NF-AT components define a family of transcription factors targeted in T-cell activation.
    Crabtree GR
    Nature 369.6480 (1994 Jun 9): 497-502.

    Nuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-Fos.
    Wagner EF
    The Journal of biological chemistry 279.25 (2004 Jun 18): 26475-80.

    Redundant expression but selective utilization of nuclear factor of activated T cells family members.
    Crabtree GR
    Journal of immunology (Baltimore, Md. : 1950) 159.6 (1997 Sep 15): 2735-40.

    NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity.
    Crabtree GR
    The Journal of biological chemistry 270.34 (1995 Aug 25): 19898-907.

    All References

    Role of the NF-ATc transcription factor in morphogenesis of cardiac valves and septum.
    Mak TW
    Nature 392.6672 (1998 Mar 12): 182-6.

    Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression.
    Crabtree GR
    Nature 383.6603 (1996 Oct 31): 837-40.

    Nuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-Fos.
    Wagner EF
    The Journal of biological chemistry 279.25 (2004 Jun 18): 26475-80.

    Redundant expression but selective utilization of nuclear factor of activated T cells family members.
    Crabtree GR
    Journal of immunology (Baltimore, Md. : 1950) 159.6 (1997 Sep 15): 2735-40.

    Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction.
    Crabtree GR
    Current biology : CB 8.10 (1998 May 7): 563-72.

    Protein kinase A negatively modulates the nuclear accumulation of NF-ATc1 by priming for subsequent phosphorylation by glycogen synthase kinase-3.
    Gardner P
    The Journal of biological chemistry 277.50 (2002 Dec 13): 48664-76.

    Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature.
    Crabtree GR
    Cell 105.7 (2001 Jun 29): 863-75.

    Nuclear factor of activated T cells (NFAT) as a new component of the signal transduction pathway in glioma cells.
    Kaminska B
    Journal of neurochemistry 71.1 (1998 Jul): 134-41.

    Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction.
    Crabtree GR
    Genes & development 11.7 (1997 Apr 1): 824-34.

    NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity.
    Crabtree GR
    The Journal of biological chemistry 270.34 (1995 Aug 25): 19898-907.

    Different nuclear signals are activated by the B cell receptor during positive versus negative signaling.
    Goodnow CC
    Immunity 6.4 (1997 Apr): 419-28.

    Selective role of NFATc3 in positive selection of thymocytes.
    Crabtree GR
    Journal of immunology (Baltimore, Md. : 1950) 179.1 (2007 Jul 1): 103-10.

    NF-AT components define a family of transcription factors targeted in T-cell activation.
    Crabtree GR
    Nature 369.6480 (1994 Jun 9): 497-502.

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