BA-F8

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$40.00
SKU: BA-F8
View product citations for antibody BA-F8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (slow, alpha- and beta-)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Padova
Positive Tested Species Reactivity: Axolotl, Human, Llama, Rodent, Xenopus
Depositors Notes: Fusion: 1986. Recognizes type 1 fibers (alpha- and beta-slow MyHC) in skeletal muscle
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH7
Immunogen: purified myosin from adult bovine atrium
Alternate Gene Names: MyHC-slow
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: Q9BE39 
Immunogen Sequence:
Entrez Gene ID: 282714 
Additional Characterization:
Antibody Registry ID: AB_10572253 
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BA-F8 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BA-F8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

27 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    IF References

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation.
    Dunn SE
    FEBS letters 391.1-2 (1996 Aug 5): 39-44.

    Tissue-specific role of the Na,K-ATPase α2 isozyme in skeletal muscle.
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Enhanced Ca2+ transport and muscle relaxation in skeletal muscle from sarcolipin-null mice.
    Gramolini AO
    American journal of physiology. Cell physiology 301.4 (2011 Oct): C841-9.

    [Use of colloid solution of In113m in clinical practice].
    Sedova TS
    Meditsinskaia radiologiia 18.8 (1973 Aug): 25-31.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry.
    Aszmann OC
    Muscle and nerve 54.2 (2016 Aug): 292-9.

    Exercise endurance capacity is markedly reduced due to impaired energy homeostasis during prolonged fasting in FABP4/5 deficient mice.
    Kurabayashi M
    BMC physiology 19.1 (2019 Mar 13): 1.

    Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice.
    Kuang S
    The Journal of biological chemistry 294.37 (2019 Sep 13): 13718-13728.

    Effects of exercise training with short-duration intermittent hypoxia on endurance performance and muscle metabolism in well-trained mice.
    Suzuki J
    Physiological reports 7.14 (2019 Jul): e14182.

    Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice.
    Kuang S
    The Journal of biological chemistry 294.37 (2019 Sep 13): 13718-13728.

    Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity.
    Stone MH
    Sports (Basel, Switzerland) 7.7 (2019 Jul 11): .

    WB References

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers.
    Tupling AR
    PloS one 8.12 (2013): e84304.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity.
    Stone MH
    Sports (Basel, Switzerland) 7.7 (2019 Jul 11): .

    IHC References

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama.
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Hedgehog regulation of superficial slow muscle fibres in Xenopus and the evolution of tetrapod trunk myogenesis.
    Hughes SM
    Development (Cambridge, England) 131.14 (2004 Jul): 3249-62.

    N-propionylated group B meningococcal polysaccharide mimics a unique epitope on group B Neisseria meningitidis.
    Ashton FE
    The Journal of experimental medicine 165.4 (1987 Apr 1): 1207-11.

    Stabilization of speckle-type POZ protein (Spop) by Daz interacting protein 1 (Dzip1) is essential for Gli turnover and the proper output of Hedgehog signaling.
    Yang J
    The Journal of biological chemistry 288.45 (2013 Nov 8): 32809-20.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama).
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama).
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Sternohyoid and diaphragm muscle form and function during postnatal development in the rat.
    O'Halloran KD
    Experimental physiology 98.9 (2013 Sep): 1386-400.

    The role of estrogen receptor-α in estrogen-mediated regulation of basal and exercise-induced Hsp70 and Hsp27 expression in rat soleus.
    Tupling AR
    Canadian journal of physiology and pharmacology 91.10 (2013 Oct): 823-29.

    The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans.
    Hepple RT
    PloS one 9.8 (2014): e103044.

    Systems-based discovery of tomatidine as a natural small molecule inhibitor of skeletal muscle atrophy.
    Adams CM
    The Journal of biological chemistry 289.21 (2014 May 23): 14913-24.

    Structural and functional characteristics of the thoracolumbar multifidus muscle in horses.
    Victorica CL
    Journal of anatomy 230.3 (2017 Mar): 398-406.

    A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.
    Demontis F
    Cell reports 28.5 (2019 Jul 30): 1268-1281.e6.

    Fidelity of muscle fibre reinnervation modulates ageing muscle impact in elderly women.
    Hepple RT
    The Journal of physiology 597.19 (2019 Oct): 5009-5023.

    All References

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama.
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Hedgehog regulation of superficial slow muscle fibres in Xenopus and the evolution of tetrapod trunk myogenesis.
    Hughes SM
    Development (Cambridge, England) 131.14 (2004 Jul): 3249-62.

    N-propionylated group B meningococcal polysaccharide mimics a unique epitope on group B Neisseria meningitidis.
    Ashton FE
    The Journal of experimental medicine 165.4 (1987 Apr 1): 1207-11.

    Stabilization of speckle-type POZ protein (Spop) by Daz interacting protein 1 (Dzip1) is essential for Gli turnover and the proper output of Hedgehog signaling.
    Yang J
    The Journal of biological chemistry 288.45 (2013 Nov 8): 32809-20.

    Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama).
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Sternohyoid and diaphragm muscle form and function during postnatal development in the rat.
    O'Halloran KD
    Experimental physiology 98.9 (2013 Sep): 1386-400.

    The role of estrogen receptor-α in estrogen-mediated regulation of basal and exercise-induced Hsp70 and Hsp27 expression in rat soleus.
    Tupling AR
    Canadian journal of physiology and pharmacology 91.10 (2013 Oct): 823-29.

    The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans.
    Hepple RT
    PloS one 9.8 (2014): e103044.

    Systems-based discovery of tomatidine as a natural small molecule inhibitor of skeletal muscle atrophy.
    Adams CM
    The Journal of biological chemistry 289.21 (2014 May 23): 14913-24.

    Structural and functional characteristics of the thoracolumbar multifidus muscle in horses.
    Victorica CL
    Journal of anatomy 230.3 (2017 Mar): 398-406.

    A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.
    Demontis F
    Cell reports 28.5 (2019 Jul 30): 1268-1281.e6.

    Fidelity of muscle fibre reinnervation modulates ageing muscle impact in elderly women.
    Hepple RT
    The Journal of physiology 597.19 (2019 Oct): 5009-5023.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation.
    Dunn SE
    FEBS letters 391.1-2 (1996 Aug 5): 39-44.

    Tissue-specific role of the Na,K-ATPase α2 isozyme in skeletal muscle.
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Enhanced Ca2+ transport and muscle relaxation in skeletal muscle from sarcolipin-null mice.
    Gramolini AO
    American journal of physiology. Cell physiology 301.4 (2011 Oct): C841-9.

    [Use of colloid solution of In113m in clinical practice].
    Sedova TS
    Meditsinskaia radiologiia 18.8 (1973 Aug): 25-31.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry.
    Aszmann OC
    Muscle and nerve 54.2 (2016 Aug): 292-9.

    Exercise endurance capacity is markedly reduced due to impaired energy homeostasis during prolonged fasting in FABP4/5 deficient mice.
    Kurabayashi M
    BMC physiology 19.1 (2019 Mar 13): 1.

    Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice.
    Kuang S
    The Journal of biological chemistry 294.37 (2019 Sep 13): 13718-13728.

    Effects of exercise training with short-duration intermittent hypoxia on endurance performance and muscle metabolism in well-trained mice.
    Suzuki J
    Physiological reports 7.14 (2019 Jul): e14182.

    Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity.
    Stone MH
    Sports (Basel, Switzerland) 7.7 (2019 Jul 11): .

    Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers.
    Tupling AR
    PloS one 8.12 (2013): e84304.

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