BA-D5

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$40.00
SKU: BA-D5
View product citations for antibody BA-D5 on CiteAb

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Available: 363

DSHB Data Sheet

Catalog Fields

Antigen: Myosin Heavy Chain Type I
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Padova
Positive Tested Species Reactivity: Bovine, Canine, Fish, Goat, Guinea Pig, Horse, Human, Lamb, Llama, Mouse, Porcine, Rabbit, Rat, Zebrafish
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH7
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: myosin heavy chain, slow, alpha- and beta-. Myosin heavy chain 7
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q9BE39 
Immunogen Sequence:
Entrez Gene ID: 282714 
Additional Characterization:
Antibody Registry ID: AB_2235587 
Additional Information: Muscle contraction. RRID:AB_2235587
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BA-D5 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BA-D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    IF References

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis.
    Sahenk Z
    Skeletal muscle 7.1 (2017 Dec 14): 27.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants.
    McClung JM
    JCI insight 3.21 (2018 Nov 2): .

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging.
    Mann M
    Cell reports 19.11 (2017 Jun 13): 2396-2409.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    WB References
    IHC References
    All References

    New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes.
    Rivero JL
    Cell and tissue research 323.2 (2006 Feb): 283-303.

    Sulf1 modulates BMP signaling and is required for somite morphogenesis and development of the horizontal myoseptum.
    Pownall ME
    Developmental biology 378.2 (2013 Jun 15): 107-21.

    Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
    Söll D
    Nucleic acids research 11.24 (1983 Dec 20): 8677-90.

    Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study.
    Peterson CA
    Journal of translational medicine 14.1 (2016 Sep 29): 284.

    Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.
    Brotto M
    The Journal of biological chemistry 291.9 (2016 Feb 26): 4308-22.

    Cellular and morphological changes with EAA supplementation before and after total knee arthroplasty.
    Dreyer HC
    Journal of applied physiology (Bethesda, Md. : 1985) 127.2 (2019 Aug 1): 531-545.

    Hypertrophic muscle growth and metabolic efficiency were impaired by chronic heat stress, improved by zilpaterol supplementation, and not affected by ractopamine supplementation in feedlot lambs1.
    Yates DT
    Journal of animal science 97.10 (2019 Oct 3): 4101-4113.

    Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock.
    Schiaffino S
    Molecular metabolism 3.1 (2014 Feb): 29-41.

    Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model.
    Soslowsky LJ
    Annals of biomedical engineering 44.10 (2016 Oct): 2901-2910.

    Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis.
    Sahenk Z
    Skeletal muscle 7.1 (2017 Dec 14): 27.

    Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants.
    McClung JM
    JCI insight 3.21 (2018 Nov 2): .

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression.
    Chen ZB
    Current medical science 39.1 (2019 Feb): 59-66.

    Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging.
    Mann M
    Cell reports 19.11 (2017 Jun 13): 2396-2409.

    Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.
    Jiang QY
    EMBO reports 20.9 (2019 Sep): e47892.

    Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods.
    Soukup T
    European journal of histochemistry : EJH 52.3 (2008 Jul-Sep): 179-90.

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

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