X-AC9

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SKU: X-AC9-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: X-AC9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: COL10A1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528175 
Uniprot ID: P08125 
RRID:  
Entrez Gene ID: 100858979 
Clonality: Monoclonal
Immunogen: Conditioned supernatant from chick chondrocytes in culture
Clone:
Immunogen Sequence: Ammonium sulfate precipitated collagen type X
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: Collagen, type X
Epitope Location or Sequence: The epitope lies near the C-terminus of the major collagenase fragment within the triple helical domain of type X collagen.
Alternate Antigen Name:
Deposit Date: 2/11/1999
Antigen Molecular Weight: 66.4 kDa
Depositor: Linsenmayer, T.F.
Antigen Sequence:
Depositor Institution: Tufts Medical School
Antigen Species: Chicken
Depositor Notes: Immunostaining requires pretreatment with 0.5% testicular hyaluronidase (500U/mg) for 1 hr at 37°C.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chicken, Cuttlefish, Human, Mouse, Zebrafish
Additional Information: RRID:AB_528175
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
X-AC9 was deposited to the DSHB by Linsenmayer, T.F. (DSHB Hybridoma Product X-AC9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    IF References

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds.
    Vargas AO
    Scientific reports 5. (2015 May 14): 9840.

    Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.
    Cancela ML
    Zebrafish 10.4 (2013 Dec): 500-9.

    Human mesenchymal stromal cells from adipose tissue of the neck.
    Kramer J
    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 269.12 (2012 Dec): 2561-70.

    Dynamic compression improves biosynthesis of human zonal chondrocytes from osteoarthritis patients.
    Klein TJ
    Osteoarthritis and cartilage 20.8 (2012 Aug): 906-15.

    FoxA family members are crucial regulators of the hypertrophic chondrocyte differentiation program.
    Lassar AB
    Developmental cell 22.5 (2012 May 15): 927-39.

    Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.
    Naus CC
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 26.12 (2011 Dec): 2911-22.

    Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction.
    Kramer J
    BMC cell biology 10. (2009 Dec 19): 92.

    Positive regulation of endochondral cartilage growth by perichondrial and periosteal calcitonin.
    Linsenmayer TF
    Endocrinology 144.5 (2003 May): 1979-83.

    Environmental regulation of type X collagen production by cultures of limb mesenchyme, mesectoderm, and sternal chondrocytes.
    Linsenmayer TF
    Developmental biology 117.1 (1986 Sep): 90-101.

    IHC References
    ELISA References

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    Epitope Map References

    Type X collagen contains two cleavage sites for a vertebrate collagenase.
    Linsenmayer TF
    The Journal of biological chemistry 261.9 (1986 Mar 25): 4184-9.

    All References

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    Transient exposure to transforming growth factor beta 3 improves the mechanical properties of mesenchymal stem cell-laden cartilage constructs in a density-dependent manner.
    Mauck RL
    Tissue engineering. Part A 15.11 (2009 Nov): 3461-72.

    Bioinspired Injectable Hydrogels Dynamically Stiffen and Contract to Promote Mechanosensing-Mediated Chondrogenic Commitment of Stem Cells.
    Katti DS
    ACS applied materials & interfaces 14.6 (2022 Feb 16): 7531-7550.

    Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds.
    Vargas AO
    Scientific reports 5. (2015 May 14): 9840.

    Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.
    Cancela ML
    Zebrafish 10.4 (2013 Dec): 500-9.

    Human mesenchymal stromal cells from adipose tissue of the neck.
    Kramer J
    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 269.12 (2012 Dec): 2561-70.

    Dynamic compression improves biosynthesis of human zonal chondrocytes from osteoarthritis patients.
    Klein TJ
    Osteoarthritis and cartilage 20.8 (2012 Aug): 906-15.

    FoxA family members are crucial regulators of the hypertrophic chondrocyte differentiation program.
    Lassar AB
    Developmental cell 22.5 (2012 May 15): 927-39.

    Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.
    Naus CC
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 26.12 (2011 Dec): 2911-22.

    Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction.
    Kramer J
    BMC cell biology 10. (2009 Dec 19): 92.

    Positive regulation of endochondral cartilage growth by perichondrial and periosteal calcitonin.
    Linsenmayer TF
    Endocrinology 144.5 (2003 May): 1979-83.

    Environmental regulation of type X collagen production by cultures of limb mesenchyme, mesectoderm, and sternal chondrocytes.
    Linsenmayer TF
    Developmental biology 117.1 (1986 Sep): 90-101.

    Type X collagen contains two cleavage sites for a vertebrate collagenase.
    Linsenmayer TF
    The Journal of biological chemistry 261.9 (1986 Mar 25): 4184-9.

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