X-AC9

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$40.00
SKU: X-AC9-s
View product citations for antibody X-AC9 on CiteAb

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Available: 32

DSHB Data Sheet

Catalog Fields

Antigen: Collagen, type X
Hybridoma Cells Available: No
Antigen Species: Chicken
Depositor: Linsenmayer, T.F.
Isotype: MIgG1
Depositors Institution: Tufts Medical School
Host Species: mouse
Depositors Notes: Immunostaining requires pretreatment with 0.5% testicular hyaluronidase (500U/mg) for 1 hr at 37°C.
Positive Tested Species Reactivity: Chicken, Cuttlefish, Human, Mouse, Zebrafish
Gene: COL10A1
Antigen Molecular Weight: 66.4 kDa
Clonality: Monoclonal
Immunogen: Conditioned supernatant from chick chondrocytes in culture
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: The epitope lies near the C-terminus of the major collagenase fragment within the triple helical domain of type X collagen.
Uniprot ID: P08125 
Immunogen Sequence: Ammonium sulfate precipitated collagen type X
Entrez Gene ID: 100858979 
Additional Information: RRID:AB_528175
Antibody Registry ID: AB_528175 
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
X-AC9 was deposited to the DSHB by Linsenmayer, T.F. (DSHB Hybridoma Product X-AC9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    IF References

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds.
    Vargas AO
    Scientific reports 5. (2015 May 14): 9840.

    Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.
    Cancela ML
    Zebrafish 10.4 (2013 Dec): 500-9.

    Human mesenchymal stromal cells from adipose tissue of the neck.
    Kramer J
    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 269.12 (2012 Dec): 2561-70.

    Dynamic compression improves biosynthesis of human zonal chondrocytes from osteoarthritis patients.
    Klein TJ
    Osteoarthritis and cartilage 20.8 (2012 Aug): 906-15.

    FoxA family members are crucial regulators of the hypertrophic chondrocyte differentiation program.
    Lassar AB
    Developmental cell 22.5 (2012 May 15): 927-39.

    Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.
    Naus CC
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 26.12 (2011 Dec): 2911-22.

    Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction.
    Kramer J
    BMC cell biology 10. (2009 Dec 19): 92.

    Positive regulation of endochondral cartilage growth by perichondrial and periosteal calcitonin.
    Linsenmayer TF
    Endocrinology 144.5 (2003 May): 1979-83.

    Environmental regulation of type X collagen production by cultures of limb mesenchyme, mesectoderm, and sternal chondrocytes.
    Linsenmayer TF
    Developmental biology 117.1 (1986 Sep): 90-101.

    IHC References
    ELISA References

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    Epitope Map References

    Type X collagen contains two cleavage sites for a vertebrate collagenase.
    Linsenmayer TF
    The Journal of biological chemistry 261.9 (1986 Mar 25): 4184-9.

    All References

    Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
    Linsenmayer TF
    The Journal of cell biology 100.2 (1985 Feb): 598-605.

    Transient exposure to transforming growth factor beta 3 improves the mechanical properties of mesenchymal stem cell-laden cartilage constructs in a density-dependent manner.
    Mauck RL
    Tissue engineering. Part A 15.11 (2009 Nov): 3461-72.

    Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds.
    Vargas AO
    Scientific reports 5. (2015 May 14): 9840.

    Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.
    Cancela ML
    Zebrafish 10.4 (2013 Dec): 500-9.

    Human mesenchymal stromal cells from adipose tissue of the neck.
    Kramer J
    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 269.12 (2012 Dec): 2561-70.

    Dynamic compression improves biosynthesis of human zonal chondrocytes from osteoarthritis patients.
    Klein TJ
    Osteoarthritis and cartilage 20.8 (2012 Aug): 906-15.

    FoxA family members are crucial regulators of the hypertrophic chondrocyte differentiation program.
    Lassar AB
    Developmental cell 22.5 (2012 May 15): 927-39.

    Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.
    Naus CC
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 26.12 (2011 Dec): 2911-22.

    Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction.
    Kramer J
    BMC cell biology 10. (2009 Dec 19): 92.

    Positive regulation of endochondral cartilage growth by perichondrial and periosteal calcitonin.
    Linsenmayer TF
    Endocrinology 144.5 (2003 May): 1979-83.

    Environmental regulation of type X collagen production by cultures of limb mesenchyme, mesectoderm, and sternal chondrocytes.
    Linsenmayer TF
    Developmental biology 117.1 (1986 Sep): 90-101.

    Type X collagen contains two cleavage sites for a vertebrate collagenase.
    Linsenmayer TF
    The Journal of biological chemistry 261.9 (1986 Mar 25): 4184-9.

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