Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works on C. elegans whole mounts. Stains the plasma membrane at cell-cell contacts. Works poorly in detecting SAX-7 on immunoblots. By western blot, CeSAX7 identifies a band of 55 kDa which may represent a post-translational cleaved product [PMID: 20405020].
Antigen Molecular Weight: Predicted: 124 (shortform) and 145 (longform) kDa; Apparent: multiple bands between 45 and 200 kDa
Predicted Species Reactivity:
Immunogen: Recombinant His6-tagged SAX-7 (C-terminal cytoplasmic domain 94 aa) fusion protein
Alternate Gene Names:
Alternate Antibody Name: SAX7, 11.2.1
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: C-terminal cytoplasmic domain 94 aa
Immunogen Sequence: Partial protein
Additional Information: SAX-7 is a homolog of the cell adhesion molecule L1/CAM which localizes most prominently to sites of cell-cell contact in the nervous system.
Recommended Applications: Immunofluorescence, Western Blot