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DSHB Data Sheet

Catalog Fields

Antigen: SAX-7
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Depositors Institution: Washington University Medical School
Host Species: mouse
Depositors Notes: Works on C. elegans whole mounts. Stains the plasma membrane at cell-cell contacts. Works poorly in detecting SAX-7 on immunoblots. By western blot, CeSAX7 identifies a band of 55 kDa which may represent a post-translational cleaved product [PMID: 20405020].
Positive Tested Species Reactivity: C. elegans
Gene: sax-7
Antigen Molecular Weight: Predicted: 124 (shortform) and 145 (longform) kDa; Apparent: multiple bands between 45 and 200 kDa
Clonality: Monoclonal
Immunogen: Recombinant His6-tagged SAX-7 (C-terminal cytoplasmic domain 94 aa) fusion protein
Epitope Mapped: Yes
Alternate Antibody Name: SAX7, 11.2.1
Epitope Location or Sequence: C-terminal cytoplasmic domain 94 aa
Myeloma Strain: P3X63Ag8.653
Immunogen Sequence: Partial protein
Uniprot ID: Q53U86 Q53U87 
Additional Information: SAX-7 is a homolog of the cell adhesion molecule L1/CAM which localizes most prominently to sites of cell-cell contact in the nervous system.
Entrez Gene ID: 177602 
Antibody Registry ID: AB_10567266 
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CeSAX7 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product CeSAX7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References
    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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