LET413

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$40.00
SKU: LET413
View product citations for antibody LET413 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: LET-413 (lap1, Scribble)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works well on C. elegans whole mounts. Stains basolateral membranes.
Antigen Molecular Weight: Predicted: 75 kDA and 77 kDa; Apparent: 76 kDa (and two faint bands at 110 and 200 kDa)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: let-413
Immunogen: Recombinant His6-tagged LET-413 (aa 460-606 of isoform a) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 5C12
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: aa 460-606 of isoform a
Uniprot ID: O61967 
Immunogen Sequence: Partial protein
Entrez Gene ID: 179171 
Additional Characterization:  
Antibody Registry ID: AB_10571452 
Additional Information: The protein lap1 encoded by let-413 is a homolog of ERBIN, DENSIN-180 and SCRIB, an epithelial basolateral membrane protein, in the LAP family of proteins.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LET413 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product LET413)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References
    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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