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Rat-Elav-7E8A10 anti-elav

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SKU: Rat-Elav-7E8A10 anti-elav
View product citations for antibody Rat-Elav-7E8A10 anti-elav on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: elav Drosophila protein; embryonic lethal abnormal vision
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: HHMI / Janelia Farm Research
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is a marker for most differentiated neuronal cells in the central and peripheral nervous system.
Antigen Molecular Weight: 50.8 kD Predicted
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: elav
Immunogen: Recombinant protein
Alternate Gene Names: elav-2, elav-3, fliJ, weg
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P16914 
Immunogen Sequence: Full length protein
Entrez Gene ID: 31000 
Additional Characterization:
Antibody Registry ID: AB_528218 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rat-Elav-7E8A10 anti-elav was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product Rat-Elav-7E8A10 anti-elav)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

39 References

  • Initial Publication
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  • Initial Publication
    IF References
    IHC References
    All References

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