Antigen: elav Drosophila protein; embryonic lethal abnormal vision
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Host Species: rat
Depositors Institution: HHMI / Janelia Farm Research
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is a marker for most differentiated neuronal cells in the central and peripheral nervous system.
Antigen Molecular Weight: 50.8 kD Predicted
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant protein
Alternate Gene Names: elav-2, elav-3, fliJ, weg
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Location or Sequence:
Uniprot ID: P16914
Immunogen Sequence: Full length protein
Entrez Gene ID: 31000
Antibody Registry ID: AB_528218
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rat-Elav-7E8A10 anti-elav was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product Rat-Elav-7E8A10 anti-elav)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).