MANDYS1(3B7)
Catalog Fields
Product Name/ID: MANDYS1(3B7)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: DMD
Ab Isotype: MIgG2a
Gene Symbol:
Entrez Gene ID: 1756
Clonality: Monoclonal
Immunogen: Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NS0 or Sp2/0
Epitope Mapped: Yes
Antigen Name: Dystrophin, exons 31/32
Epitope Location or Sequence: exons 31/32, aa 1431-1505 (repeats 10 and 11 of the central rod domain)
Alternate Antigen Name:
Deposit Date: 10/26/2005
Antigen Molecular Weight: Predicted: 427 kDa; Apparent: 427 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: human
Depositor Notes: This antibody does not work with Xenopus
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Human, Mouse, Pig, Rabbit
Additional Information:
Additional Characterization: http://www.glennmorris.org.uk/datasheets/MANDYS1(3B7).doc
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS1(3B7) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS1(3B7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin.
Davies KE
FEBS letters 262.2 (1990 Mar 26): 237-40.