IVD3(1)A9

(0) No Reviews yet
$40.00
SKU: IVD3(1)A9-s
View product citations for antibody IVD3(1)A9 on CiteAb

In Stock

Available: 166

DSHB Data Sheet

Catalog Fields

Antigen: alpha sarcoglycan
Hybridoma Cells Available: No
Antigen Species: Rabbit
Depositor: Campbell, K.P.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Canine, Human, Mouse, Rabbit, Rat, Sheep
Depositors Notes:
Antigen Molecular Weight: Predicted: 40 kDa; Apparent: 50 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SGCA
Immunogen: Isolated membranes from skeletal muscle.
Alternate Gene Names: ADL, DAG2, 50DAG, DMDA2, LGMD2D, SCARMD1, adhalin
Alternate Antibody Name: IVD31
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q28686 
Immunogen Sequence: Full length protein
Entrez Gene ID: 100009178 
Additional Characterization:  
Antibody Registry ID: AB_2185657 
Additional Information: The alpha sarcoglican is a component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.This antibody doesn't work on canine western blots. IVD3(1)A9 works under nonreducing conditions (and not under reducing conditions) for western blotting. One reference states that the epitope is located in the C-terminal region [PMID: 8040315]. RRID:AB_2185657
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IVD3(1)A9 was deposited to the DSHB by Campbell, K.P. (DSHB Hybridoma Product IVD3(1)A9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

35 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Identification of novel proteins unique to either transverse tubules (TS28) or the sarcolemma (SL50) in rabbit skeletal muscle.
    Campbell KP
    The Journal of cell biology 110.4 (1990 Apr): 1173-85.

    Identification of novel proteins unique to either transverse tubules (TS28) or the sarcolemma (SL50) in rabbit skeletal muscle.
    Campbell KP
    The Journal of cell biology 110.4 (1990 Apr): 1173-85.

    Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma.
    Campbell KP
    The Journal of cell biology 112.1 (1991 Jan): 135-48.

    Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle.
    Campbell KP
    Neuron 7.3 (1991 Sep): 499-508.

    Deficiency of the 50K dystrophin-associated glycoprotein in severe childhood autosomal recessive muscular dystrophy.
    Campbell KP
    Nature 359.6393 (1992 Sep 24): 320-2.

    Dystrophin-glycoprotein complex and laminin colocalize to the sarcolemma and transverse tubules of cardiac muscle.
    Jorgensen AO
    Circulation research 72.2 (1993 Feb): 349-60.

    Abnormal expression of dystrophin-associated proteins in Fukuyama-type congenital muscular dystrophy.
    Campbell KP
    Lancet (London, England) 341.8844 (1993 Feb 27): 521-2.

    Duchenne muscular dystrophy: deficiency of dystrophin-associated proteins in the sarcolemma.
    Campbell KP
    Neurology 43.4 (1993 Apr): 795-800.

    Mild deficiency of dystrophin-associated proteins in Becker muscular dystrophy patients having in-frame deletions in the rod domain of dystrophin.
    Campbell KP
    American journal of human genetics 53.2 (1993 Aug): 409-16.

    Deficiency of dystrophin-associated proteins in Duchenne muscular dystrophy patients lacking COOH-terminal domains of dystrophin.
    Fardeau M
    The Journal of clinical investigation 92.2 (1993 Aug): 866-71.

    Severe childhood autosomal recessive muscular dystrophy with the deficiency of the 50 kDa dystrophin-associated glycoprotein maps to chromosome 13q12.
    Weissenbach J
    Human molecular genetics 2.9 (1993 Sep): 1423-8.

    Differential expression of dystrophin, utrophin and dystrophin-associated proteins in peripheral nerve.
    Campbell KP
    FEBS letters 334.3 (1993 Nov 22): 281-5.

    Immunohistochemical analysis of dystrophin-associated proteins in Becker/Duchenne muscular dystrophy with huge in-frame deletions in the NH2-terminal and rod domains of dystrophin.
    Reinert P
    The Journal of clinical investigation 93.1 (1994 Jan): 99-105.

    Expression of dystrophin-associated proteins in dystrophin-positive muscle fibers (revertants) in Duchenne muscular dystrophy.
    Campbell KP
    Neuromuscular disorders : NMD 4.2 (1994 Mar): 115-20.

    Genetic heterogeneity of severe childhood autosomal recessive muscular dystrophy with adhalin (50 kDa dystrophin-associated glycoprotein) deficiency.
    Fardeau M
    Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie 317.1 (1994 Jan): 70-6.

    Assessment of the 50-kDa dystrophin-associated glycoprotein in Brazilian patients with severe childhood autosomal recessive muscular dystrophy.
    Campbell KP
    Journal of the neurological sciences 123.1-2 (1994 May): 122-8.

    Abnormal expression of laminin suggests disturbance of sarcolemma-extracellular matrix interaction in Japanese patients with autosomal recessive muscular dystrophy deficient in adhalin.
    Campbell KP
    The Journal of clinical investigation 94.2 (1994 Aug): 601-6.

    A role for dystrophin-associated glycoproteins and utrophin in agrin-induced AChR clustering.
    Scheller RH
    Cell 77.5 (1994 Jun 3): 663-74.

    Expression of dystrophin-associated glycoproteins and utrophin in carriers of Duchenne muscular dystrophy.
    Dubowitz V
    Neuromuscular disorders : NMD 4.5-6 (1994 Sep-Nov): 401-9.

    Laminin abnormality in severe childhood autosomal recessive muscular dystrophy.
    Mitsui T
    Laboratory investigation; a journal of technical methods and pathology 72.6 (1995 Jun): 715-22.

    A common missense mutation in the adhalin gene in three unrelated Brazilian families with a relatively mild form of autosomal recessive limb-girdle muscular dystrophy.
    Zatz M
    Human molecular genetics 4.7 (1995 Jul): 1163-7.

    The expression of dystrophin-associated glycoproteins during skeletal muscle degeneration and regeneration. An immunofluorescence study.
    Cullen MJ
    Journal of neuropathology and experimental neurology 54.4 (1995 Jul): 557-69.

    Adhalin gene mutations in patients with autosomal recessive childhood onset muscular dystrophy with adhalin deficiency.
    Shin S
    The Journal of clinical investigation 96.3 (1995 Sep): 1202-7.

    Beta-sarcoglycan: characterization and role in limb-girdle muscular dystrophy linked to 4q12.
    Slaughter C
    Nature genetics 11.3 (1995 Nov): 257-65.

    Merosin-negative congenital muscular dystrophy associated with extensive brain abnormalities.
    Campbell KP
    Neurology 45.11 (1995 Nov): 2084-9.

    Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice.
    Richter EA
    The Journal of physiology 594.17 (2016 Sep 1): 4997-5008.

    Purinergic receptors expressed in human skeletal muscle fibres.
    Thaning P
    Purinergic signalling 8.2 (2012 Jun): 255-64.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    Orthogonal analysis of dystrophin protein and mRNA as a surrogate outcome for drug development.
    Hoffman EP
    Biomarkers in medicine 13.14 (2019 Oct): 1209-1225.

    WB References
    IHC References
    IP References
    All References

    Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy.
    Huchet C
    PloS one 9.10 (2014): e110371.

    Identification of novel proteins unique to either transverse tubules (TS28) or the sarcolemma (SL50) in rabbit skeletal muscle.
    Campbell KP
    The Journal of cell biology 110.4 (1990 Apr): 1173-85.

    Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma.
    Campbell KP
    The Journal of cell biology 112.1 (1991 Jan): 135-48.

    Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle.
    Campbell KP
    Neuron 7.3 (1991 Sep): 499-508.

    Deficiency of the 50K dystrophin-associated glycoprotein in severe childhood autosomal recessive muscular dystrophy.
    Campbell KP
    Nature 359.6393 (1992 Sep 24): 320-2.

    Dystrophin-glycoprotein complex and laminin colocalize to the sarcolemma and transverse tubules of cardiac muscle.
    Jorgensen AO
    Circulation research 72.2 (1993 Feb): 349-60.

    Abnormal expression of dystrophin-associated proteins in Fukuyama-type congenital muscular dystrophy.
    Campbell KP
    Lancet (London, England) 341.8844 (1993 Feb 27): 521-2.

    Duchenne muscular dystrophy: deficiency of dystrophin-associated proteins in the sarcolemma.
    Campbell KP
    Neurology 43.4 (1993 Apr): 795-800.

    Mild deficiency of dystrophin-associated proteins in Becker muscular dystrophy patients having in-frame deletions in the rod domain of dystrophin.
    Campbell KP
    American journal of human genetics 53.2 (1993 Aug): 409-16.

    Deficiency of dystrophin-associated proteins in Duchenne muscular dystrophy patients lacking COOH-terminal domains of dystrophin.
    Fardeau M
    The Journal of clinical investigation 92.2 (1993 Aug): 866-71.

    Severe childhood autosomal recessive muscular dystrophy with the deficiency of the 50 kDa dystrophin-associated glycoprotein maps to chromosome 13q12.
    Weissenbach J
    Human molecular genetics 2.9 (1993 Sep): 1423-8.

    Differential expression of dystrophin, utrophin and dystrophin-associated proteins in peripheral nerve.
    Campbell KP
    FEBS letters 334.3 (1993 Nov 22): 281-5.

    Immunohistochemical analysis of dystrophin-associated proteins in Becker/Duchenne muscular dystrophy with huge in-frame deletions in the NH2-terminal and rod domains of dystrophin.
    Reinert P
    The Journal of clinical investigation 93.1 (1994 Jan): 99-105.

    Expression of dystrophin-associated proteins in dystrophin-positive muscle fibers (revertants) in Duchenne muscular dystrophy.
    Campbell KP
    Neuromuscular disorders : NMD 4.2 (1994 Mar): 115-20.

    Genetic heterogeneity of severe childhood autosomal recessive muscular dystrophy with adhalin (50 kDa dystrophin-associated glycoprotein) deficiency.
    Fardeau M
    Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie 317.1 (1994 Jan): 70-6.

    Assessment of the 50-kDa dystrophin-associated glycoprotein in Brazilian patients with severe childhood autosomal recessive muscular dystrophy.
    Campbell KP
    Journal of the neurological sciences 123.1-2 (1994 May): 122-8.

    Abnormal expression of laminin suggests disturbance of sarcolemma-extracellular matrix interaction in Japanese patients with autosomal recessive muscular dystrophy deficient in adhalin.
    Campbell KP
    The Journal of clinical investigation 94.2 (1994 Aug): 601-6.

    A role for dystrophin-associated glycoproteins and utrophin in agrin-induced AChR clustering.
    Scheller RH
    Cell 77.5 (1994 Jun 3): 663-74.

    Expression of dystrophin-associated glycoproteins and utrophin in carriers of Duchenne muscular dystrophy.
    Dubowitz V
    Neuromuscular disorders : NMD 4.5-6 (1994 Sep-Nov): 401-9.

    Laminin abnormality in severe childhood autosomal recessive muscular dystrophy.
    Mitsui T
    Laboratory investigation; a journal of technical methods and pathology 72.6 (1995 Jun): 715-22.

    A common missense mutation in the adhalin gene in three unrelated Brazilian families with a relatively mild form of autosomal recessive limb-girdle muscular dystrophy.
    Zatz M
    Human molecular genetics 4.7 (1995 Jul): 1163-7.

    The expression of dystrophin-associated glycoproteins during skeletal muscle degeneration and regeneration. An immunofluorescence study.
    Cullen MJ
    Journal of neuropathology and experimental neurology 54.4 (1995 Jul): 557-69.

    Adhalin gene mutations in patients with autosomal recessive childhood onset muscular dystrophy with adhalin deficiency.
    Shin S
    The Journal of clinical investigation 96.3 (1995 Sep): 1202-7.

    Beta-sarcoglycan: characterization and role in limb-girdle muscular dystrophy linked to 4q12.
    Slaughter C
    Nature genetics 11.3 (1995 Nov): 257-65.

    Merosin-negative congenital muscular dystrophy associated with extensive brain abnormalities.
    Campbell KP
    Neurology 45.11 (1995 Nov): 2084-9.

    Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice.
    Richter EA
    The Journal of physiology 594.17 (2016 Sep 1): 4997-5008.

    Purinergic receptors expressed in human skeletal muscle fibres.
    Thaning P
    Purinergic signalling 8.2 (2012 Jun): 255-64.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    Orthogonal analysis of dystrophin protein and mRNA as a surrogate outcome for drug development.
    Hoffman EP
    Biomarkers in medicine 13.14 (2019 Oct): 1209-1225.

    Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle.
    Campbell KP
    FEBS letters 283.2 (1991 Jun 3): 230-4.

    Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy.
    Burkin DJ
    Molecular therapy : the journal of the American Society of Gene Therapy 23.8 (2015 Aug): 1285-1297.

    Deficiency of merosin in dystrophic dy mice and genetic linkage of laminin M chain gene to dy locus.
    Campbell KP
    The Journal of biological chemistry 269.19 (1994 May 13): 13729-32.

    Deficiency of the 50 kDa dystrophin associated glycoprotein (adhalin) in severe autosomal recessive muscular dystrophies in children native from European countries.
    Campbell KP
    Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie 316.8 (1993 Aug): 799-804.

    Congenital muscular dystrophy with merosin deficiency.
    Fardeau M
    Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie 317.4 (1994 Apr): 351-7.

    Deficiency of the 50 kDa dystrophin-associated glycoprotein and abnormal expression of utrophin in two south Asian cousins with variable expression of severe childhood autosomal recessive muscular dystrophy.
    Dubowitz V
    Neuromuscular disorders : NMD 4.2 (1994 Mar): 121-9.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review