IVD3(1)A9
Catalog Fields
Clone ID/Product Name: IVD3(1)A9
Available to For-Profits: Yes
Alternate Antibody Name: IVD31
Gene Symbol: SGCA
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2185657
Uniprot ID: Q28686
Entrez Gene ID: 100009178
Clonality: Monoclonal
Immunogen: Isolated membranes from skeletal muscle.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: alpha sarcoglycan
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/26/2004
Antigen Molecular Weight: Predicted: 40 kDa; Apparent: 50 kDa
Depositor: Campbell, K.P.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Rabbit
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Canine, Human, Mouse, Rabbit, Rat, Sheep
Additional Information: The alpha sarcoglican is a component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.This antibody doesn't work on canine western blots. IVD3(1)A9 works under nonreducing conditions (and not under reducing conditions) for western blotting. One reference states that the epitope is located in the C-terminal region [PMID: 8040315]. RRID:AB_2185657
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IVD3(1)A9 was deposited to the DSHB by Campbell, K.P. (DSHB Hybridoma Product IVD3(1)A9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
35 References
Initial Publication
Identification of novel proteins unique to either transverse tubules (TS28) or the sarcolemma (SL50) in rabbit skeletal muscle.
Campbell KP
The Journal of cell biology 110.4 (1990 Apr): 1173-85.