P4C4-B10

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$40.00
SKU: P4C4-B10
View product citations for antibody P4C4-B10 on CiteAb

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Available: 97

DSHB Data Sheet

Catalog Fields

Antigen: Myc protein/ Diminutive
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Eisenman, R.N.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 79.3 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Myc
Immunogen:
Alternate Gene Names: dMyc, dm, d-myc, c-MYC
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q9W4S7 
Immunogen Sequence:
Entrez Gene ID: 31310 
Additional Characterization:  
Antibody Registry ID:  
Additional Information: FBgn0262656
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4C4-B10 was deposited to the DSHB by Eisenman, R.N. (DSHB Hybridoma Product P4C4-B10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Ras1 promotes cellular growth in the Drosophila wing.
    Edgar BA
    Cell 100.4 (2000 Feb 18): 435-46.

    IF References

    Ras1 promotes cellular growth in the Drosophila wing.
    Edgar BA
    Cell 100.4 (2000 Feb 18): 435-46.

    Interactions between Ras1, dMyc, and dPI3K signaling in the developing Drosophila wing.
    Edgar BA
    Genes & development 16.17 (2002 Sep 1): 2286-99.

    Drosophila Myc, a novel modifier suppresses the poly(Q) toxicity by modulating the level of CREB binding protein and histone acetylation.
    Sarkar S
    Neurobiology of disease 63. (2014 Mar): 48-61.

    The Drosophila F box protein archipelago regulates dMyc protein levels in vivo.
    Hariharan IK
    Current biology : CB 14.11 (2004 Jun 8): 965-74.

    The Drosophila ubiquitin-specific protease Puffyeye regulates dMyc-mediated growth.
    Eisenman RN
    Development (Cambridge, England) 140.23 (2013 Dec): 4776-87.

    dMyc is required for larval growth and endoreplication in Drosophila.
    Eisenman RN
    Development (Cambridge, England) 131.10 (2004 May): 2317-27.

    The steroid hormone ecdysone controls systemic growth by repressing dMyc function in Drosophila fat cells.
    Léopold P
    Developmental cell 18.6 (2010 Jun 15): 1012-21.

    Inverse regulation of two classic Hippo pathway target genes in Drosophila by the dimerization hub protein Ctp.
    Moberg K
    Scientific reports 6. (2016 Mar 14): 22726.

    Sunspot, a link between Wingless signaling and endoreplication in Drosophila.
    Akiyama T
    Development (Cambridge, England) 137.10 (2010 May): 1755-64.

    WB References

    The Drosophila ubiquitin-specific protease Puffyeye regulates dMyc-mediated growth.
    Eisenman RN
    Development (Cambridge, England) 140.23 (2013 Dec): 4776-87.

    The Drosophila F box protein archipelago regulates dMyc protein levels in vivo.
    Hariharan IK
    Current biology : CB 14.11 (2004 Jun 8): 965-74.

    dMyc is required for larval growth and endoreplication in Drosophila.
    Eisenman RN
    Development (Cambridge, England) 131.10 (2004 May): 2317-27.

    snoRNAs are a novel class of biologically relevant Myc targets.
    Gallant P
    BMC biology 13. (2015 Apr 16): 25.

    PAF1 complex component Leo1 helps recruit <i>Drosophila</i> Myc to promoters.
    Gallant P
    Proceedings of the National Academy of Sciences of the United States of America 114.44 (2017 Oct 31): E9224-E9232.

    Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster.
    Barrio R
    Scientific reports 5. (2015 Jul 22): 12383.

    IHC References

    Drosophila Minus is required for cell proliferation and influences Cyclin E turnover.
    Cohen SM
    Genes & development 23.17 (2009 Sep 1): 1998-2003.

    IP References

    snoRNAs are a novel class of biologically relevant Myc targets.
    Gallant P
    BMC biology 13. (2015 Apr 16): 25.

    PAF1 complex component Leo1 helps recruit <i>Drosophila</i> Myc to promoters.
    Gallant P
    Proceedings of the National Academy of Sciences of the United States of America 114.44 (2017 Oct 31): E9224-E9232.

    All References

    Drosophila Minus is required for cell proliferation and influences Cyclin E turnover.
    Cohen SM
    Genes & development 23.17 (2009 Sep 1): 1998-2003.

    Ras1 promotes cellular growth in the Drosophila wing.
    Edgar BA
    Cell 100.4 (2000 Feb 18): 435-46.

    Interactions between Ras1, dMyc, and dPI3K signaling in the developing Drosophila wing.
    Edgar BA
    Genes & development 16.17 (2002 Sep 1): 2286-99.

    Drosophila Myc, a novel modifier suppresses the poly(Q) toxicity by modulating the level of CREB binding protein and histone acetylation.
    Sarkar S
    Neurobiology of disease 63. (2014 Mar): 48-61.

    The Drosophila F box protein archipelago regulates dMyc protein levels in vivo.
    Hariharan IK
    Current biology : CB 14.11 (2004 Jun 8): 965-74.

    The Drosophila ubiquitin-specific protease Puffyeye regulates dMyc-mediated growth.
    Eisenman RN
    Development (Cambridge, England) 140.23 (2013 Dec): 4776-87.

    dMyc is required for larval growth and endoreplication in Drosophila.
    Eisenman RN
    Development (Cambridge, England) 131.10 (2004 May): 2317-27.

    The steroid hormone ecdysone controls systemic growth by repressing dMyc function in Drosophila fat cells.
    Léopold P
    Developmental cell 18.6 (2010 Jun 15): 1012-21.

    Inverse regulation of two classic Hippo pathway target genes in Drosophila by the dimerization hub protein Ctp.
    Moberg K
    Scientific reports 6. (2016 Mar 14): 22726.

    Sunspot, a link between Wingless signaling and endoreplication in Drosophila.
    Akiyama T
    Development (Cambridge, England) 137.10 (2010 May): 1755-64.

    snoRNAs are a novel class of biologically relevant Myc targets.
    Gallant P
    BMC biology 13. (2015 Apr 16): 25.

    PAF1 complex component Leo1 helps recruit <i>Drosophila</i> Myc to promoters.
    Gallant P
    Proceedings of the National Academy of Sciences of the United States of America 114.44 (2017 Oct 31): E9224-E9232.

    Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster.
    Barrio R
    Scientific reports 5. (2015 Jul 22): 12383.

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