Antigen: Wingless protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Cohen, S.M.
Host Species: mouse
Depositors Institution: EMBL
Positive Tested Species Reactivity: Chicken, Drosophila, Lizard, Mouse, Planaria
Depositors Notes: Antibody is specific for Drosophila Wingless protein
Antigen Molecular Weight: Predicted: 46-51 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Wingless (Drosophila, C-terminus a.a. 3-468); recombinant.
Alternate Gene Names: Br/DWint-1/ Dint-1/ Dm Wg/ Dm-1/ WNT/Wnt, Wnt-1/ int-1/ spdl/
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Ag8
Epitope Location or Sequence:
Uniprot ID: P09615
Entrez Gene ID: 34009
Antibody Registry ID: AB_528512
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D4 was deposited to the DSHB by Cohen, S.M. (DSHB Hybridoma Product 4D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Gel Supershift References