4D4

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$40.00
SKU: 4D4
View product citations for antibody 4D4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Wingless protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Cohen, S.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL
Positive Tested Species Reactivity: Chicken, Drosophila, Lizard, Mouse, Planaria
Depositors Notes: Antibody is specific for Drosophila Wingless protein
Antigen Molecular Weight: Predicted: 46-51 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: wg
Immunogen: Wingless (Drosophila, C-terminus a.a. 3-468); recombinant.
Alternate Gene Names: Br/DWint-1/ Dint-1/ Dm Wg/ Dm-1/ WNT/Wnt, Wnt-1/ int-1/ spdl/
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Ag8
Epitope Location or Sequence:
Uniprot ID: P09615 
Immunogen Sequence:
Entrez Gene ID: 34009 
Additional Characterization:
Antibody Registry ID: AB_528512 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D4 was deposited to the DSHB by Cohen, S.M. (DSHB Hybridoma Product 4D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

53 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Roles of N-glycosylation and lipidation in Wg secretion and signaling.
    Lin X
    Developmental biology 364.1 (2012 Apr 1): 32-41.

    Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development.
    Affolter M
    Development (Cambridge, England) 141.2 (2014 Jan): 472-80.

    A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling.
    Zhu AJ
    G3 (Bethesda, Md.) 2.12 (2012 Dec): 1563-75.

    A genome-wide RNAi screen identifies regulators of cholesterol-modified hedgehog secretion in Drosophila.
    Thérond P
    PloS one 7.3 (2012): e33665.

    Cohesin controls planar cell polarity by regulating the level of the seven-pass transmembrane cadherin Flamingo.
    Uemura T
    Genes to cells : devoted to molecular & cellular mechanisms 17.6 (2012 Jun): 509-24.

    Notch and Mef2 synergize to promote proliferation and metastasis through JNK signal activation in Drosophila.
    Artavanis-Tsakonas S
    The EMBO journal 31.13 (2012 Jun 29): 2895-907.

    The Hippo pathway regulates Wnt/beta-catenin signaling.
    Attisano L
    Developmental cell 18.4 (2010 Apr 20): 579-91.

    The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.
    Liu QX
    Scientific reports 6. (2016 Jun 15): 27981.

    Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema.
    Alibardi L
    Acta histochemica 119.3 (2017 Apr): 211-219.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila.
    Cho KO
    Cell death & disease 10.8 (2019 Jul 22): 564.

    WB References

    Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema.
    Alibardi L
    Acta histochemica 119.3 (2017 Apr): 211-219.

    Drosophila heparan sulfate 6-O-endosulfatase Sulf1 facilitates wingless (Wg) protein degradation.
    Nakato H
    The Journal of biological chemistry 288.7 (2013 Feb 15): 5081-9.

    Drosophila segment polarity gene product porcupine stimulates the posttranslational N-glycosylation of wingless in the endoplasmic reticulum.
    Kadowaki T
    The Journal of biological chemistry 277.15 (2002 Apr 12): 12816-23.

    Coop functions as a corepressor of Pangolin and antagonizes Wingless signaling.
    Basler K
    Genes & development 24.9 (2010 May): 881-6.

    Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells.
    Schwientek T
    Proteomics 11.22 (2011 Nov): 4397-410.

    Signal transduction by the Fat cytoplasmic domain.
    Irvine KD
    Development (Cambridge, England) 140.4 (2013 Feb): 831-42.

    Drosophila heparan sulfate 6-O-endosulfatase Sulf1 facilitates wingless (Wg) protein degradation.
    Nakato H
    The Journal of biological chemistry 288.7 (2013 Feb 15): 5081-9.

    Subcellular distribution of Wnt-1 at adherens junctions and actin-rich densities in endothelial cells.
    Coan DE
    Experimental cell research 288.2 (2003 Aug 15): 335-43.

    Roles of N-glycosylation and lipidation in Wg secretion and signaling.
    Lin X
    Developmental biology 364.1 (2012 Apr 1): 32-41.

    ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila.
    Cho KO
    Cell death & disease 10.8 (2019 Jul 22): 564.

    IHC References

    Drosophila pupal abdomen immunohistochemistry.
    Yoder JH
    Journal of visualized experiments : JoVE .56 (2011 Oct 2): .

    A critical role for endocytosis in Wnt signaling.
    Nusse R
    BMC cell biology 7. (2006 Jul 6): 28.

    Loss of Drosophila Myb interrupts the progression of chromosome condensation.
    Lipsick JS
    Nature cell biology 9.5 (2007 May): 581-7.

    Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons.
    Budnik V
    The Journal of biological chemistry 287.20 (2012 May 11): 16820-34.

    Roles of N-glycosylation and lipidation in Wg secretion and signaling.
    Lin X
    Developmental biology 364.1 (2012 Apr 1): 32-41.

    Enhancer-associated H3K4 monomethylation by Trithorax-related, the Drosophila homolog of mammalian Mll3/Mll4.
    Shilatifard A
    Genes & development 26.23 (2012 Dec 1): 2604-20.

    Roles of N-glycosylation and lipidation in Wg secretion and signaling.
    Lin X
    Developmental biology 364.1 (2012 Apr 1): 32-41.

    The core protein of glypican Dally-like determines its biphasic activity in wingless morphogen signaling.
    Lin X
    Developmental cell 17.4 (2009 Oct): 470-81.

    Spenito and Split ends act redundantly to promote Wingless signaling.
    Cadigan KM
    Developmental biology 314.1 (2008 Feb 1): 100-11.

    Drosophila Ndfip is a novel regulator of Notch signaling.
    Kumar S
    Cell death and differentiation 18.7 (2011 Jul): 1150-60.

    Asymmetric distribution of Spalt in Drosophila wing squamous and columnar epithelia ensures correct cell morphogenesis.
    Shen J
    Scientific reports 6. (2016 Jul 25): 30236.

    The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.
    Liu QX
    Scientific reports 6. (2016 Jun 15): 27981.

    Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription.
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema.
    Alibardi L
    Acta histochemica 119.3 (2017 Apr): 211-219.

    IP References

    Regulation of Notch signalling by non-visual beta-arrestin.
    Artavanis-Tsakonas S
    Nature cell biology 7.12 (2005 Dec): 1191-201.

    Roles of N-glycosylation and lipidation in Wg secretion and signaling.
    Lin X
    Developmental biology 364.1 (2012 Apr 1): 32-41.

    The core protein of glypican Dally-like determines its biphasic activity in wingless morphogen signaling.
    Lin X
    Developmental cell 17.4 (2009 Oct): 470-81.

    FFPE References
    Gel Supershift References
    All References

    Drosophila pupal abdomen immunohistochemistry.
    Yoder JH
    Journal of visualized experiments : JoVE .56 (2011 Oct 2): .

    A critical role for endocytosis in Wnt signaling.
    Nusse R
    BMC cell biology 7. (2006 Jul 6): 28.

    Loss of Drosophila Myb interrupts the progression of chromosome condensation.
    Lipsick JS
    Nature cell biology 9.5 (2007 May): 581-7.

    Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons.
    Budnik V
    The Journal of biological chemistry 287.20 (2012 May 11): 16820-34.

    Roles of N-glycosylation and lipidation in Wg secretion and signaling.
    Lin X
    Developmental biology 364.1 (2012 Apr 1): 32-41.

    Enhancer-associated H3K4 monomethylation by Trithorax-related, the Drosophila homolog of mammalian Mll3/Mll4.
    Shilatifard A
    Genes & development 26.23 (2012 Dec 1): 2604-20.

    The core protein of glypican Dally-like determines its biphasic activity in wingless morphogen signaling.
    Lin X
    Developmental cell 17.4 (2009 Oct): 470-81.

    Spenito and Split ends act redundantly to promote Wingless signaling.
    Cadigan KM
    Developmental biology 314.1 (2008 Feb 1): 100-11.

    Drosophila Ndfip is a novel regulator of Notch signaling.
    Kumar S
    Cell death and differentiation 18.7 (2011 Jul): 1150-60.

    Asymmetric distribution of Spalt in Drosophila wing squamous and columnar epithelia ensures correct cell morphogenesis.
    Shen J
    Scientific reports 6. (2016 Jul 25): 30236.

    The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.
    Liu QX
    Scientific reports 6. (2016 Jun 15): 27981.

    Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription.
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema.
    Alibardi L
    Acta histochemica 119.3 (2017 Apr): 211-219.

    Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development.
    Affolter M
    Development (Cambridge, England) 141.2 (2014 Jan): 472-80.

    A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling.
    Zhu AJ
    G3 (Bethesda, Md.) 2.12 (2012 Dec): 1563-75.

    A genome-wide RNAi screen identifies regulators of cholesterol-modified hedgehog secretion in Drosophila.
    Thérond P
    PloS one 7.3 (2012): e33665.

    Cohesin controls planar cell polarity by regulating the level of the seven-pass transmembrane cadherin Flamingo.
    Uemura T
    Genes to cells : devoted to molecular & cellular mechanisms 17.6 (2012 Jun): 509-24.

    Notch and Mef2 synergize to promote proliferation and metastasis through JNK signal activation in Drosophila.
    Artavanis-Tsakonas S
    The EMBO journal 31.13 (2012 Jun 29): 2895-907.

    The Hippo pathway regulates Wnt/beta-catenin signaling.
    Attisano L
    Developmental cell 18.4 (2010 Apr 20): 579-91.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila.
    Cho KO
    Cell death & disease 10.8 (2019 Jul 22): 564.

    Drosophila heparan sulfate 6-O-endosulfatase Sulf1 facilitates wingless (Wg) protein degradation.
    Nakato H
    The Journal of biological chemistry 288.7 (2013 Feb 15): 5081-9.

    Drosophila segment polarity gene product porcupine stimulates the posttranslational N-glycosylation of wingless in the endoplasmic reticulum.
    Kadowaki T
    The Journal of biological chemistry 277.15 (2002 Apr 12): 12816-23.

    Coop functions as a corepressor of Pangolin and antagonizes Wingless signaling.
    Basler K
    Genes & development 24.9 (2010 May): 881-6.

    Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells.
    Schwientek T
    Proteomics 11.22 (2011 Nov): 4397-410.

    Signal transduction by the Fat cytoplasmic domain.
    Irvine KD
    Development (Cambridge, England) 140.4 (2013 Feb): 831-42.

    Subcellular distribution of Wnt-1 at adherens junctions and actin-rich densities in endothelial cells.
    Coan DE
    Experimental cell research 288.2 (2003 Aug 15): 335-43.

    Regulation of Notch signalling by non-visual beta-arrestin.
    Artavanis-Tsakonas S
    Nature cell biology 7.12 (2005 Dec): 1191-201.

    Antagonistic interactions between wingless and decapentaplegic responsible for dorsal-ventral pattern in the Drosophila Leg.
    Cohen SM
    Science (New York, N.Y.) 273.5280 (1996 Sep 6): 1373-7.

    Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors.
    Trembleau A
    Development (Cambridge, England) 130.9 (2003 May): 1867-76.

    The WNT7b promoter is regulated by TTF-1, GATA6, and Foxa2 in lung epithelium.
    Morrisey EE
    The Journal of biological chemistry 277.23 (2002 Jun 7): 21061-70.

    Sonic hedgehog mutations identified in holoprosencephaly patients can act in a dominant negative manner.
    Robbins DJ
    Human genetics 125.1 (2009 Feb): 95-103.

    The role of Drosophila heparan sulfate 6-O-endosulfatase in sulfation compensation.
    Nakato H
    The Journal of biological chemistry 288.9 (2013 Mar 1): 6574-82.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Role of the cellular prion protein in oligodendrocyte precursor cell proliferation and differentiation in the developing and adult mouse CNS.
    del Río JA
    PloS one 7.4 (2012): e33872.

    Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development.
    Cohen SM
    Current biology : CB 24.13 (2014 Jul 7): 1476-84.

    Scoring criteria for response latency and habituation in electrodermal research: a study in the context of the orienting response.
    Barry RJ
    Psychophysiology 27.1 (1990 Jan): 94-100.

    Wounded embryonic corneas exhibit nonfibrotic regeneration and complete innervation.
    Lwigale PY
    Investigative ophthalmology & visual science 54.9 (2013 Sep 24): 6334-44.



    . (): .

    Skeletal muscle neural progenitor cells exhibit properties of NG2-glia.
    Delbono O
    Experimental cell research 319.1 (2013 Jan 1): 45-63.

    The bHLH factors Dpn and members of the E(spl) complex mediate the function of Notch signalling regulating cell proliferation during wing disc development.
    Baonza A
    Biology open 1.7 (2012 Jul 15): 667-76.

    Early postnatal in vivo gliogenesis from nestin-lineage progenitors requires cdk5.
    Eisch AJ
    PloS one 8.8 (2013): e72819.

    The Ecdysone receptor constrains wingless expression to pattern cell cycle across the Drosophila wing margin in a Cyclin B-dependent manner.
    Quinn LM
    BMC developmental biology 13. (2013 Jul 13): 28.

    Wingless secretion requires endosome-to-Golgi retrieval of Wntless/Evi/Sprinter by the retromer complex.
    Vincent JP
    Nature cell biology 10.2 (2008 Feb): 170-7.

    The role of Bro1- domain-containing protein Myopic in endosomal trafficking of Wnt/Wingless.
    Verheyen EM
    Developmental biology 392.1 (2014 Aug 1): 93-107.

    Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo.
    Tjian R
    Proceedings of the National Academy of Sciences of the United States of America 106.1 (2009 Jan 6): 55-60.

    Cholesterol modification is necessary for controlled planar long-range activity of Hedgehog in Drosophila epithelia.
    Thérond PP
    Development (Cambridge, England) 133.3 (2006 Feb): 407-18.

    The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility.
    Wodarz A
    PLoS genetics 10.7 (2014 Jul): e1004443.

    Enhancer-PRE communication contributes to the expansion of gene expression domains in proliferating primordia.
    Milán M
    Development (Cambridge, England) 138.15 (2011 Aug): 3125-34.

    The BEAF insulator regulates genes involved in cell polarity and neoplastic growth.
    Corces VG
    Developmental biology 369.1 (2012 Sep 1): 124-32.

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