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View product citations for antibody PMY-2A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: puromycin
Hybridoma Cells Available: Yes
Antigen Species: Streptomyces alboniger
Depositor: Yewdell, Jonathan
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Rat
Depositors Notes: Fusion date: 07/21/2010. Fixation, 4% paraformaldehyde. The antigen puromycin inhibits translation by covalently associating with nascent chain within the ribosome. 2A4 allows puromycin detection in this condition. 2A4 also detects any puromycylated peptide. Medium: RPMI 1640 / Glutamine / Na pyruvate / 10% fetal bovine serum / Penicillin-streptomycin /gentamycin
Antigen Molecular Weight: 0.5kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: Puromycin (coupled to KLH) from Streptomyces alboniger bacterium. Chemical Composition: 3?-(?-Amino-p-methoxyhydrocinnamamido)-3?-deoxy-N,N-dimethyladenosine, 2HC1.
Alternate Gene Names:
Alternate Antibody Name: clone 4A12
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2619605 
Additional Information: Puromycin is a structural analog of aminoacyl tRNAs that can be incorporated into nascent polypeptide chain and blocks elongation. This antibody is used to detect the subcellular localization of actively translating ribosomes by the ribopuromycylation method (RPM), and localizes puromycylated nascent chains on ribosomes immobilized by chain elongation inhibitors in living cells prior to processing for intracellular immunofluorescence and/or western blots. RRID:AB_2619605
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PMY-2A4 was deposited to the DSHB by Yewdell, Jonathan (DSHB Hybridoma Product PMY-2A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Nuclear translation visualized by ribosome-bound nascent chain puromycylation.
    Yewdell JW
    The Journal of cell biology 197.1 (2012 Apr 2): 45-57.

    IF References
    WB References
    IHC References
    All References

    Heads or tails? Differential translational regulation in cercarial heads and tails of schistosome worms.
    Jolly ER
    PloS one 14.10 (2019): e0224358.

    Nuclear translation visualized by ribosome-bound nascent chain puromycylation.
    Yewdell JW
    The Journal of cell biology 197.1 (2012 Apr 2): 45-57.

    Emetine optimally facilitates nascent chain puromycylation and potentiates the ribopuromycylation method (RPM) applied to inert cells.
    Yewdell JW
    Histochemistry and cell biology 139.3 (2013 Mar): 501-4.

    Virus-mediated compartmentalization of the host translational machinery.
    Parker JS
    mBio 5.5 (2014 Sep 16): e01463-14.

    Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.
    Brogna S
    RNA (New York, N.Y.) 19.12 (2013 Dec): 1669-83.

    Spatiotemporal analysis of hepatitis C virus infection.
    Randall G
    PLoS pathogens 11.3 (2015 Mar): e1004758.

    Polysomes identified by live imaging of nascent peptides are stalled in hippocampal and cortical neurites.
    Sossin WS
    Learning & memory (Cold Spring Harbor, N.Y.) 26.9 (2019 Sep): 351-362.

    Requirement of Neuronal Ribosome Synthesis for Growth and Maintenance of the Dendritic Tree.
    Hetman M
    The Journal of biological chemistry 291.11 (2016 Mar 11): 5721-39.

    Structure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution.
    Hegde RS
    Cell 157.7 (2014 Jun 19): 1632-43.

    Hepatoprotective role of Sestrin2 against chronic ER stress.
    Lee JH
    Nature communications 5. (2014 Jun 20): 4233.

    Actin dynamics tune the integrated stress response by regulating eukaryotic initiation factor 2α dephosphorylation.
    Marciniak SJ
    eLife 4. (2015 Mar 16): .

    A broad-spectrum antiviral molecule, QL47, selectively inhibits eukaryotic translation.
    Yang PL
    The Journal of biological chemistry 295.6 (2020 Feb 7): 1694-1703.

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