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View product citations for antibody PMY-2A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: puromycin
Hybridoma Cells Available: Yes
Antigen Species: Streptomyces alboniger
Depositor: Yewdell, Jonathan
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Rat
Depositors Notes: Fusion date: 07/21/2010. Fixation, 4% paraformaldehyde. The antigen puromycin inhibits translation by covalently associating with nascent chain within the ribosome. 2A4 allows puromycin detection in this condition. 2A4 also detects any puromycylated peptide. Medium: RPMI 1640 / Glutamine / Na pyruvate / 10% fetal bovine serum / Penicillin-streptomycin /gentamycin
Antigen Molecular Weight: 0.5kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Immunogen: Puromycin (coupled to KLH) from Streptomyces alboniger bacterium. Chemical Composition: 3?-(?-Amino-p-methoxyhydrocinnamamido)-3?-deoxy-N,N-dimethyladenosine, 2HC1.
Alternate Gene Names:
Alternate Antibody Name: clone 4A12
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2619605 
Additional Information: Puromycin is a structural analog of aminoacyl tRNAs that can be incorporated into nascent polypeptide chain and blocks elongation. This antibody is used to detect the subcellular localization of actively translating ribosomes by the ribopuromycylation method (RPM), and localizes puromycylated nascent chains on ribosomes immobilized by chain elongation inhibitors in living cells prior to processing for intracellular immunofluorescence and/or western blots. RRID:AB_2619605
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PMY-2A4 was deposited to the DSHB by Yewdell, Jonathan (DSHB Hybridoma Product PMY-2A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Nuclear translation visualized by ribosome-bound nascent chain puromycylation.
    Yewdell JW
    The Journal of cell biology 197.1 (2012 Apr 2): 45-57.

    IF References
    WB References
    All References

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