WVID1(9C5)

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$40.00
SKU: WVID1(9C5)
View product citations for antibody WVID1(9C5) on CiteAb

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Available: 57

DSHB Data Sheet

Catalog Fields

Antigen: bone sialoprotein II
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Solursh, M. / Franzen, A.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Acorn worm, Canine, Cuttlefish, Horseshoe crab, Human, Mouse, Quail, Rabbit, Rat
Depositors Notes: Antigen chemical properties: recognizes bone sialoprotein II (BSPII) by ELISA. IHC note: stains rat bone.
Antigen Molecular Weight: 35.2 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ibsp
Immunogen: bone sialoproteins I and II
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P13839 
Immunogen Sequence: Full length
Entrez Gene ID: 24477 
Additional Characterization:  
Antibody Registry ID: AB_2121529 
Additional Information: RRID:AB_2121529
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
WVID1(9C5) was deposited to the DSHB by Solursh, M. / Franzen, A. (DSHB Hybridoma Product WVID1(9C5))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

38 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Osteogenic cell response to calcium aluminate-based cement.
    de Oliveira PT
    International endodontic journal 50.8 (2017 Aug): 771-779.

    Bioactive-glass ceramic with two crystalline phases (BioS-2P) for bone tissue engineering.
    Luiz Rosa A
    Biomedical materials (Bristol, England) 12.4 (2017 Aug 9): 045018.

    Calcium chloride-enriched calcium aluminate cement promotes in vitro osteogenesis.
    de Oliveira PT
    International endodontic journal 51.6 (2018 Jun): 674-683.

    Oxidative nanopatterning of titanium surfaces promotes production and extracellular accumulation of osteopontin.
    Oliveira PT
    Brazilian dental journal 22.3 (2011): 179-84.

    Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering.
    Alsberg E
    Biomaterials 161. (2018 Apr): 240-255.

    Long-period gratings in photonic crystal fiber as an optofluidic label-free biosensor.
    Du H
    Biosensors & bioelectronics 26.12 (2011 Aug 15): 4774-8.

    Effects of the association between a calcium hydroxide paste and 0.4% chlorhexidine on the development of the osteogenic phenotype in vitro.
    de Oliveira PT
    Journal of endodontics 34.12 (2008 Dec): 1485-9.

    Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells.
    Nanci A
    Biomaterials 25.3 (2004 Feb): 403-13.

    Enhancement of in vitro osteogenesis on titanium by chemically produced nanotopography.
    Nanci A
    Journal of biomedical materials research. Part A 80.3 (2007 Mar 1): 554-64.

    Treatment with a growth factor-protein mixture inhibits formation of mineralized nodules in osteogenic cell cultures grown on titanium.
    de Oliveira PT
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.3 (2009 Mar): 265-76.

    Differentiation plasticity of chondrocytes derived from mouse embryonic stem cells.
    Rohwedel J
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4617-28.

    Reversible suppression of in vitro biomineralization by activation of protein kinase A.
    Midura RJ
    The Journal of biological chemistry 275.15 (2000 Apr 14): 11082-91.

    In vivo matrix-guided human mesenchymal stem cells.
    Rohwedel J
    Cellular and molecular life sciences : CMLS 63.5 (2006 Mar): 616-26.

    Osteoblasts derived from induced pluripotent stem cells form calcified structures in scaffolds both in vitro and in vivo.
    Majka SM
    Stem cells (Dayton, Ohio) 29.2 (2011 Feb): 206-16.

    In vitro osteogenesis on a highly bioactive glass-ceramic (Biosilicate).
    de Oliveira PT
    Journal of biomedical materials research. Part A 82.3 (2007 Sep 1): 545-57.

    Progression of osteogenic cell cultures grown on microtopographic titanium coated with calcium phosphate and functionalized with a type I collagen-derived peptide.
    de Oliveira PT
    Journal of periodontology 84.8 (2013 Aug): 1199-210.

    Human mesenchymal stromal cells from adipose tissue of the neck.
    Kramer J
    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 269.12 (2012 Dec): 2561-70.

    Effects of enamel matrix derivative and transforming growth factor-β1 on connective tissue growth factor in human periodontal ligament fibroblasts.
    Pischon N
    Journal of periodontology 86.4 (2015 Apr): 569-77.

    Influence of discrete and continuous culture conditions on human mesenchymal stem cell lineage choice in RGD concentration gradient hydrogels.
    Becker ML
    Biomacromolecules 14.9 (2013 Sep 9): 3047-54.

    Mesenchymal Stem Cells Repress Osteoblast Differentiation Under Osteogenic-Inducing Conditions.
    Rosa AL
    Journal of cellular biochemistry 116.12 (2015 Dec): 2896-902.

    Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases.
    Robinson WF
    Research in veterinary science 77.2 (2004 Oct): 131-41.

    Nanoscale oxidative patterning of metallic surfaces to modulate cell activity and fate.
    Nanci A
    Nano letters 9.2 (2009 Feb): 659-65.

    In vitro osteogenesis on a microstructured titanium surface with additional submicron-scale topography.
    de Oliveira PT
    Clinical oral implants research 18.3 (2007 Jun): 333-44.

    Progression of osteogenic cell cultures grown on microtopographic titanium coated with calcium phosphate and functionalized with a type I collagen-derived peptide.
    de Oliveira PT
    Journal of periodontology 84.8 (2013 Aug): 1199-210.

    Early expression of bone matrix proteins in osteogenic cell cultures.
    Nanci A
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.5 (2003 May): 633-41.

    Derivation and characterization of chondrocytes from embryonic stem cells in vitro.
    Rohwedel J
    Methods in molecular biology (Clifton, N.J.) 330. (2006): 171-90.

    Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction.
    Kramer J
    BMC cell biology 10. (2009 Dec 19): 92.

    Preadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxide.
    Bombonato-Prado KF
    Molecular biology reports 48.1 (2021 Jan): 13-20.

    WB References

    Activation of integrin alpha(V)beta(3) regulates cell adhesion and migration to bone sialoprotein.
    Plow EF
    Experimental cell research 254.2 (2000 Feb 1): 299-308.

    Reversible suppression of in vitro biomineralization by activation of protein kinase A.
    Midura RJ
    The Journal of biological chemistry 275.15 (2000 Apr 14): 11082-91.

    IHC References

    The Wnt/β-catenin signaling pathway is regulated by titanium with nanotopography to induce osteoblast differentiation.
    Beloti MM
    Colloids and surfaces. B, Biointerfaces 184. (2019 Dec 1): 110513.

    The nature and significance of invertebrate cartilages revisited: distribution and histology of cartilage and cartilage-like tissues within the Metazoa.
    Hall BK
    Zoology (Jena, Germany) 107.4 (2004): 261-73.

    Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts.
    Milz S
    Anticancer research 24.6 (2004 Nov-Dec): 3743-8.

    Emdogain regulation of cellular differentiation in wounded rat periodontium.
    McCulloch CA
    Journal of periodontal research 38.2 (2003 Apr): 164-74.

    Effects of transforming growth factor beta1 on bonelike tissue formation in three-dimensional cell culture. II: Osteoblastic differentiation.
    Schulz MB
    Tissue engineering 10.9-10 (2004 Sep-Oct): 1414-25.

    Osteoporosis-like bone conditions affect osseointegration of implants.
    Schneider GB
    The International journal of oral & maxillofacial implants 19.5 (2004 Sep-Oct): 687-94.

    Specific amelogenin gene splice products have signaling effects on cells in culture and in implants in vivo.
    Healy KE
    The Journal of biological chemistry 275.52 (2000 Dec 29): 41263-72.

    Strain-guided mineralization in the bone-PDL-cementum complex of a rat periodontium.
    Ho S
    Bone reports 3. (2015 Dec 1): 20-31.

    The Effects of Stably Tethered BMP-2 on MC3T3-E1 Preosteoblasts Encapsulated in a PEG Hydrogel.
    Bryant SJ
    Biomacromolecules 22.3 (2021 Mar 8): 1065-1079.

    All References

    The Wnt/β-catenin signaling pathway is regulated by titanium with nanotopography to induce osteoblast differentiation.
    Beloti MM
    Colloids and surfaces. B, Biointerfaces 184. (2019 Dec 1): 110513.

    The nature and significance of invertebrate cartilages revisited: distribution and histology of cartilage and cartilage-like tissues within the Metazoa.
    Hall BK
    Zoology (Jena, Germany) 107.4 (2004): 261-73.

    Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts.
    Milz S
    Anticancer research 24.6 (2004 Nov-Dec): 3743-8.

    Emdogain regulation of cellular differentiation in wounded rat periodontium.
    McCulloch CA
    Journal of periodontal research 38.2 (2003 Apr): 164-74.

    Effects of transforming growth factor beta1 on bonelike tissue formation in three-dimensional cell culture. II: Osteoblastic differentiation.
    Schulz MB
    Tissue engineering 10.9-10 (2004 Sep-Oct): 1414-25.

    Osteoporosis-like bone conditions affect osseointegration of implants.
    Schneider GB
    The International journal of oral & maxillofacial implants 19.5 (2004 Sep-Oct): 687-94.

    Specific amelogenin gene splice products have signaling effects on cells in culture and in implants in vivo.
    Healy KE
    The Journal of biological chemistry 275.52 (2000 Dec 29): 41263-72.

    Strain-guided mineralization in the bone-PDL-cementum complex of a rat periodontium.
    Ho S
    Bone reports 3. (2015 Dec 1): 20-31.

    The Effects of Stably Tethered BMP-2 on MC3T3-E1 Preosteoblasts Encapsulated in a PEG Hydrogel.
    Bryant SJ
    Biomacromolecules 22.3 (2021 Mar 8): 1065-1079.

    Osteogenic cell response to calcium aluminate-based cement.
    de Oliveira PT
    International endodontic journal 50.8 (2017 Aug): 771-779.

    Bioactive-glass ceramic with two crystalline phases (BioS-2P) for bone tissue engineering.
    Luiz Rosa A
    Biomedical materials (Bristol, England) 12.4 (2017 Aug 9): 045018.

    Calcium chloride-enriched calcium aluminate cement promotes in vitro osteogenesis.
    de Oliveira PT
    International endodontic journal 51.6 (2018 Jun): 674-683.

    Oxidative nanopatterning of titanium surfaces promotes production and extracellular accumulation of osteopontin.
    Oliveira PT
    Brazilian dental journal 22.3 (2011): 179-84.

    Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering.
    Alsberg E
    Biomaterials 161. (2018 Apr): 240-255.

    Long-period gratings in photonic crystal fiber as an optofluidic label-free biosensor.
    Du H
    Biosensors & bioelectronics 26.12 (2011 Aug 15): 4774-8.

    Effects of the association between a calcium hydroxide paste and 0.4% chlorhexidine on the development of the osteogenic phenotype in vitro.
    de Oliveira PT
    Journal of endodontics 34.12 (2008 Dec): 1485-9.

    Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells.
    Nanci A
    Biomaterials 25.3 (2004 Feb): 403-13.

    Enhancement of in vitro osteogenesis on titanium by chemically produced nanotopography.
    Nanci A
    Journal of biomedical materials research. Part A 80.3 (2007 Mar 1): 554-64.

    Treatment with a growth factor-protein mixture inhibits formation of mineralized nodules in osteogenic cell cultures grown on titanium.
    de Oliveira PT
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.3 (2009 Mar): 265-76.

    Differentiation plasticity of chondrocytes derived from mouse embryonic stem cells.
    Rohwedel J
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4617-28.

    Reversible suppression of in vitro biomineralization by activation of protein kinase A.
    Midura RJ
    The Journal of biological chemistry 275.15 (2000 Apr 14): 11082-91.

    In vivo matrix-guided human mesenchymal stem cells.
    Rohwedel J
    Cellular and molecular life sciences : CMLS 63.5 (2006 Mar): 616-26.

    Osteoblasts derived from induced pluripotent stem cells form calcified structures in scaffolds both in vitro and in vivo.
    Majka SM
    Stem cells (Dayton, Ohio) 29.2 (2011 Feb): 206-16.

    In vitro osteogenesis on a highly bioactive glass-ceramic (Biosilicate).
    de Oliveira PT
    Journal of biomedical materials research. Part A 82.3 (2007 Sep 1): 545-57.

    Progression of osteogenic cell cultures grown on microtopographic titanium coated with calcium phosphate and functionalized with a type I collagen-derived peptide.
    de Oliveira PT
    Journal of periodontology 84.8 (2013 Aug): 1199-210.

    Human mesenchymal stromal cells from adipose tissue of the neck.
    Kramer J
    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 269.12 (2012 Dec): 2561-70.

    Effects of enamel matrix derivative and transforming growth factor-β1 on connective tissue growth factor in human periodontal ligament fibroblasts.
    Pischon N
    Journal of periodontology 86.4 (2015 Apr): 569-77.

    Influence of discrete and continuous culture conditions on human mesenchymal stem cell lineage choice in RGD concentration gradient hydrogels.
    Becker ML
    Biomacromolecules 14.9 (2013 Sep 9): 3047-54.

    Mesenchymal Stem Cells Repress Osteoblast Differentiation Under Osteogenic-Inducing Conditions.
    Rosa AL
    Journal of cellular biochemistry 116.12 (2015 Dec): 2896-902.

    Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases.
    Robinson WF
    Research in veterinary science 77.2 (2004 Oct): 131-41.

    Nanoscale oxidative patterning of metallic surfaces to modulate cell activity and fate.
    Nanci A
    Nano letters 9.2 (2009 Feb): 659-65.

    In vitro osteogenesis on a microstructured titanium surface with additional submicron-scale topography.
    de Oliveira PT
    Clinical oral implants research 18.3 (2007 Jun): 333-44.

    Early expression of bone matrix proteins in osteogenic cell cultures.
    Nanci A
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.5 (2003 May): 633-41.

    Derivation and characterization of chondrocytes from embryonic stem cells in vitro.
    Rohwedel J
    Methods in molecular biology (Clifton, N.J.) 330. (2006): 171-90.

    Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction.
    Kramer J
    BMC cell biology 10. (2009 Dec 19): 92.

    Preadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxide.
    Bombonato-Prado KF
    Molecular biology reports 48.1 (2021 Jan): 13-20.

    Activation of integrin alpha(V)beta(3) regulates cell adhesion and migration to bone sialoprotein.
    Plow EF
    Experimental cell research 254.2 (2000 Feb 1): 299-308.

    Temporal studies on the tissue compartmentalization of bone sialoprotein (BSP), osteopontin (OPN), and SPARC protein during bone formation in vitro.
    Sodek J
    Journal of cellular physiology 152.3 (1992 Sep): 467-77.

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