WVID1(9C5)
Catalog Fields
Product Name/ID: WVID1(9C5)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Ibsp
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2121529
Uniprot ID: P13839
Entrez Gene ID: 24477
Clonality: Monoclonal
Immunogen: bone sialoproteins I and II
Clone:
Immunogen Sequence: Full length
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: bone sialoprotein II
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/22/1988
Antigen Molecular Weight: 35.2 kDa
Depositor: Solursh, M. / Franzen, A.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: rat
Depositor Notes: Antigen chemical properties: recognizes bone sialoprotein II (BSPII) by ELISA. IHC note: stains rat bone.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Acorn worm, Canine, Cuttlefish, Horseshoe crab, Human, Mouse, Quail, Rabbit, Rat
Additional Information: RRID:AB_2121529
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
WVID1(9C5) was deposited to the DSHB by Solursh, M. / Franzen, A. (DSHB Hybridoma Product WVID1(9C5))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
38 References
Initial Publication
Temporal studies on the tissue compartmentalization of bone sialoprotein (BSP), osteopontin (OPN), and SPARC protein during bone formation in vitro.
Sodek J
Journal of cellular physiology 152.3 (1992 Sep): 467-77.