AON-1

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$40.00
SKU: AON-1
View product citations for antibody AON-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Osteonectin (SPARC)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Termine, J.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Bone Research Branch, NIDR, NIH
Positive Tested Species Reactivity: Acorn worm, Bovine, Canine, Human, Monkey, Pig, Rabbit, Rat
Depositors Notes: The isotype of this monoclonal antibody was originally reported as IgG3 (PMID: 2476206). This antibody recognizes osteonectin (SPARC)-containing cartilages in invertebrate species. See PMID: 16351944.
Antigen Molecular Weight: 42 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: SPARC
Immunogen: Purified bovine osteonectin (SPARC)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: x63-AG8.653
Epitope Location or Sequence: The epitope bound by AON-1 is cryptic and often requires tissue pretreatment for exposure. See PMID: 19435834 & 2476206.
Uniprot ID: P13213 
Immunogen Sequence: Full length protein
Entrez Gene ID: 282077 
Additional Characterization:
Antibody Registry ID: AB_528421 
Additional Information: RRID:AB_528421
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AON-1 was deposited to the DSHB by Termine, J.D. (DSHB Hybridoma Product AON-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • All References
  • Initial Publication

    Monoclonal antibodies against osteonectin show conservation of epitopes across species.
    Termine JD
    Calcified tissue international 45.2 (1989 Aug): 74-80.

    IF References
    WB References

    Monoclonal antibodies against osteonectin show conservation of epitopes across species.
    Termine JD
    Calcified tissue international 45.2 (1989 Aug): 74-80.

    Osteonectin is a Schwann cell-secreted factor that promotes retinal ganglion cell survival and process outgrowth.
    Taylor JS
    The European journal of neuroscience 21.10 (2005 May): 2611-23.

    IHC References

    A new approach to demonstrate cellular activity in bone formation adjacent to implants.
    Albrektsson T
    Journal of biomedical materials research 51.2 (2000 Aug): 280-91.

    Bone-tissue formation and integration of titanium implants: an evaluation with newly developed enzyme and immunohistochemical techniques.
    Albrektsson T
    Clinical implant dentistry and related research 1.1 (1999): 33-40.

    Monoclonal antibodies against osteonectin show conservation of epitopes across species.
    Termine JD
    Calcified tissue international 45.2 (1989 Aug): 74-80.

    Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases.
    Robinson WF
    Research in veterinary science 77.2 (2004 Oct): 131-41.

    Inflammation-dependent expression of SPARC during development of chronic pancreatitis in WBN/Kob rats and a microarray gene expression analysis.
    Graf R
    Physiological genomics 38.2 (2009 Jul 9): 196-204.

    Autologous tenocyte therapy for experimental Achilles tendinopathy in a rabbit model.
    Zheng MH
    Tissue engineering. Part A 17.15-16 (2011 Aug): 2037-48.

    Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds.
    Dhert WJ
    Tissue engineering. Part C, Methods 18.1 (2012 Jan): 33-44.

    Effects of transforming growth factor beta1 on bonelike tissue formation in three-dimensional cell culture. II: Osteoblastic differentiation.
    Schulz MB
    Tissue engineering 10.9-10 (2004 Sep-Oct): 1414-25.

    A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues.
    Gawlitta D
    Advanced healthcare materials 8.19 (2019 Oct): e1900979.

    IP References

    Monoclonal antibodies against osteonectin show conservation of epitopes across species.
    Termine JD
    Calcified tissue international 45.2 (1989 Aug): 74-80.

    ELISA References

    Monoclonal antibodies against osteonectin show conservation of epitopes across species.
    Termine JD
    Calcified tissue international 45.2 (1989 Aug): 74-80.

    FFPE References
    All References

    A new approach to demonstrate cellular activity in bone formation adjacent to implants.
    Albrektsson T
    Journal of biomedical materials research 51.2 (2000 Aug): 280-91.

    Bone-tissue formation and integration of titanium implants: an evaluation with newly developed enzyme and immunohistochemical techniques.
    Albrektsson T
    Clinical implant dentistry and related research 1.1 (1999): 33-40.

    Monoclonal antibodies against osteonectin show conservation of epitopes across species.
    Termine JD
    Calcified tissue international 45.2 (1989 Aug): 74-80.

    Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases.
    Robinson WF
    Research in veterinary science 77.2 (2004 Oct): 131-41.

    Inflammation-dependent expression of SPARC during development of chronic pancreatitis in WBN/Kob rats and a microarray gene expression analysis.
    Graf R
    Physiological genomics 38.2 (2009 Jul 9): 196-204.

    Autologous tenocyte therapy for experimental Achilles tendinopathy in a rabbit model.
    Zheng MH
    Tissue engineering. Part A 17.15-16 (2011 Aug): 2037-48.

    Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds.
    Dhert WJ
    Tissue engineering. Part C, Methods 18.1 (2012 Jan): 33-44.

    Effects of transforming growth factor beta1 on bonelike tissue formation in three-dimensional cell culture. II: Osteoblastic differentiation.
    Schulz MB
    Tissue engineering 10.9-10 (2004 Sep-Oct): 1414-25.

    A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues.
    Gawlitta D
    Advanced healthcare materials 8.19 (2019 Oct): e1900979.

    Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue.
    Bürgi U
    Thyroid : official journal of the American Thyroid Association 7.3 (1997 Jun): 347-56.

    Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment.
    Motlík J
    Physiological research 60.3 (2011): 559-71.

    Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis.
    Miosge N
    Cell stem cell 4.4 (2009 Apr 3): 324-35.

    Correlation between in vitro expansion-related cell stiffening and differentiation potential of human mesenchymal stem cells.
    Jedlicka SS
    Differentiation; research in biological diversity 90.1-3 (2015 Jul-Oct): 1-15.

    Osteonectin is a Schwann cell-secreted factor that promotes retinal ganglion cell survival and process outgrowth.
    Taylor JS
    The European journal of neuroscience 21.10 (2005 May): 2611-23.

    The nature and significance of invertebrate cartilages revisited: distribution and histology of cartilage and cartilage-like tissues within the Metazoa.
    Hall BK
    Zoology (Jena, Germany) 107.4 (2004): 261-73.

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