Antibody Name: AON-1
Antigen:Osteonectin (SPARC)

IHC of stromal cells stained with DSHB antibody AON-1 against osteonectin (brown). Image credit: Iris Pennings, D. Gawlitta Laboratory, UMC Utrecht.
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1, kappa light chain
Myeloma Strain: x63-AG8.653
Antibody Registry / RRID: AB_528421
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Purified bovine osteonectin (SPARC)
Immunogen Sequence: Full length protein
Epitope Mapped: No
Epitope Location / Seq: The epitope bound by AON-1 is cryptic and often requires tissue pretreatment for exposure. See PMID: 19435834 & 2476206.
Clone: —
Additional Info: RRID:AB_528421
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Acorn worm, Bovine, Canine, Human, Monkey, Pig, Rabbit, Rat
Predicted Reactivity: —
Depositor
Depositor Name: J.D. Termine
Institution: NIDR, NIH, Bone Research Branch
Deposit Date: July 13, 1990
Depositor Notes: The isotype of this monoclonal antibody was originally reported as IgG3 (PMID: 2476206). This antibody recognizes osteonectin (SPARC)-containing cartilages in invertebrate species. See PMID: 16351944.
Recommended Applications
ELISA
FFPE
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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