B4-78
Catalog Fields
Antigen: alkaline phosphatase, tissue-nonspecific
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Katzmann, J.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Mayo Clinic, Rochester
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Specific for bone and liver ALP (does not react with intestinal or placental ALP; kidney ALP not tested).
Predicted Species Reactivity:
Gene: ALPL
Immunogen: Purified human bone alkaline phosphatase
Alternate Gene Names: AP-TNAP, TNSALP, HOPS
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P05186
Immunogen Sequence: Full length protein
Entrez Gene ID: 249
Antibody Registry ID: AB_528075
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B4-78 was deposited to the DSHB by Katzmann, J.A. (DSHB Hybridoma Product B4-78)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
14 References
Initial Publication
Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase.
O'Brien JF
Clinical chemistry 31.3 (1985 Mar): 381-5.