B4-78
Catalog Fields
Antigen: alkaline phosphatase, tissue-nonspecific
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Katzmann, J.A.
Isotype: MIgG1, kappa light chain
Depositors Institution: Mayo Clinic, Rochester
Host Species: mouse
Depositors Notes: Specific for bone and liver ALP (does not react with intestinal or placental ALP; kidney ALP not tested).
Positive Tested Species Reactivity: Human, Mouse, Rat
Gene: ALPL
Antigen Molecular Weight: Predicted: 47, 49, and 53 kDa;Apparent: 75 kDa
Alternate Gene Names: AP-TNAP, TNSALP, HOPS
Immunogen: Purified human bone alkaline phosphatase
Clonality: Monoclonal
Myeloma Strain: NS1
Immunogen Sequence: Full length protein
Uniprot ID: P05186
Entrez Gene ID: 249
Antibody Registry ID: AB_528075
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B4-78 was deposited to the DSHB by Katzmann, J.A. (DSHB Hybridoma Product B4-78)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase.
O'Brien JF
Clinical chemistry 31.3 (1985 Mar): 381-5.