B4-78

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$40.00
SKU: B4-78
View product citations for antibody B4-78 on CiteAb

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Available: 21

DSHB Data Sheet

Catalog Fields

Antigen: alkaline phosphatase, tissue-nonspecific
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Katzmann, J.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Mayo Clinic, Rochester
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Specific for bone and liver ALP (does not react with intestinal or placental ALP; kidney ALP not tested).
Antigen Molecular Weight: Predicted: 47, 49, and 53 kDa;Apparent: 75 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ALPL
Immunogen: Purified human bone alkaline phosphatase
Alternate Gene Names: AP-TNAP, TNSALP, HOPS
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P05186 
Immunogen Sequence: Full length protein
Entrez Gene ID: 249 
Additional Characterization:
Antibody Registry ID: AB_528075 
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B4-78 was deposited to the DSHB by Katzmann, J.A. (DSHB Hybridoma Product B4-78)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • All References
  • Initial Publication
    IF References

    Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells.
    Gimble JM
    Journal of cellular physiology 154.2 (1993 Feb): 317-28.

    Circulating osteoblast-lineage cells in humans.
    Khosla S
    The New England journal of medicine 352.19 (2005 May 12): 1959-66.

    Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment.
    Ando K
    Blood 107.5 (2006 Mar 1): 1878-87.

    Effects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures.
    Rosa AL
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.7 (2008 Jul): 629-38.

    Treatment with a growth factor-protein mixture inhibits formation of mineralized nodules in osteogenic cell cultures grown on titanium.
    de Oliveira PT
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.3 (2009 Mar): 265-76.

    A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways.
    Vogel V
    Scientific reports 6. (2016 May 9): 25104.

    Tissue-nonspecific alkaline phosphatase is activated in enterocytes by oxidative stress via changes in glycosylation.
    Sánchez de Medina F
    Inflammatory bowel diseases 17.2 (2011 Feb): 543-56.

    WB References
    IHC References
    FACS References

    Circulating osteoblast-lineage cells in humans.
    Khosla S
    The New England journal of medicine 352.19 (2005 May 12): 1959-66.

    cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
    de Boer J
    Proceedings of the National Academy of Sciences of the United States of America 105.20 (2008 May 20): 7281-6.

    All References

    Induction of alkaline phosphatase in the inflamed intestine: a novel pharmacological target for inflammatory bowel disease.
    Zarzuelo A
    Biochemical pharmacology 68.12 (2004 Dec 15): 2317-26.

    Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells.
    Gimble JM
    Journal of cellular physiology 154.2 (1993 Feb): 317-28.

    Circulating osteoblast-lineage cells in humans.
    Khosla S
    The New England journal of medicine 352.19 (2005 May 12): 1959-66.

    Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment.
    Ando K
    Blood 107.5 (2006 Mar 1): 1878-87.

    Effects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures.
    Rosa AL
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.7 (2008 Jul): 629-38.

    Treatment with a growth factor-protein mixture inhibits formation of mineralized nodules in osteogenic cell cultures grown on titanium.
    de Oliveira PT
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.3 (2009 Mar): 265-76.

    A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways.
    Vogel V
    Scientific reports 6. (2016 May 9): 25104.

    Tissue-nonspecific alkaline phosphatase is activated in enterocytes by oxidative stress via changes in glycosylation.
    Sánchez de Medina F
    Inflammatory bowel diseases 17.2 (2011 Feb): 543-56.

    Notch1 represses osteogenic pathways in aortic valve cells.
    Srivastava D
    Journal of molecular and cellular cardiology 47.6 (2009 Dec): 828-34.

    Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats.
    Sánchez de Medina F
    Pharmacological research 66.2 (2012 Aug): 144-53.

    Runx2/Cbfa1, but not loss of myocardin, is required for smooth muscle cell lineage reprogramming toward osteochondrogenesis.
    Giachelli CM
    Journal of cellular biochemistry 110.4 (2010 Jul 1): 935-47.

    cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
    de Boer J
    Proceedings of the National Academy of Sciences of the United States of America 105.20 (2008 May 20): 7281-6.

    Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase.
    O'Brien JF
    Clinical chemistry 31.3 (1985 Mar): 381-5.

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