B4-78
Catalog Fields
Antigen: alkaline phosphatase, tissue-nonspecific
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Katzmann, J.A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Institution: Mayo Clinic, Rochester
Antigen Molecular Weight: Predicted: 47, 49, and 53 kDa;Apparent: 75 kDa
Depositors Notes: Specific for bone and liver ALP (does not react with intestinal or placental ALP; kidney ALP not tested).
Immunogen: Purified human bone alkaline phosphatase
Gene: ALPL
Alternate Antibody Name:
Alternate Gene Names: AP-TNAP, TNSALP, HOPS
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: No
Uniprot ID: P05186
Epitope Location or Sequence:
Entrez Gene ID: 249
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528075
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B4-78 was deposited to the DSHB by Katzmann, J.A. (DSHB Hybridoma Product B4-78)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase.
O'Brien JF
Clinical chemistry 31.3 (1985 Mar): 381-5.