A4.840

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$40.00
SKU: A4.840
View product citations for antibody A4.840 on CiteAb

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Available: 109

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (human slow fibers)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baxter Lab for Stem Cell Biology, Stanford University
Positive Tested Species Reactivity: C. elegans, Chicken, Fish, Human, Rodent
Depositors Notes: Stains first developmental MyHCI and the epitope on slow MHC is different from that of mAbs A4.951 and N2.261 [PMID 7687223]
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH7
Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle
Alternate Gene Names: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 653
Epitope Location or Sequence: C-terminal excluding the first 100 aa of the N-terminal
Uniprot ID: P12883 
Immunogen Sequence:
Entrez Gene ID: 4625 
Additional Characterization:
Antibody Registry ID: AB_528384 
Additional Information: A4.480 may be used to stain skeletal muscle paraffin sections. High pH antigen retrieval is required. See PUBMED ID: 24924099 .
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.840 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.840)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

36 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Gene expression and fiber type variations in repeated vastus lateralis biopsies.
    Svensson MB
    Muscle and nerve 52.5 (2015 Nov): 812-7.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Length-dependent axo-terminal degeneration at the neuromuscular synapses of type II muscle in SOD1 mice.
    Farah MH
    Neuroscience 312. (2016 Jan 15): 179-89.

    Thermal acclimation to cold alters myosin content and contractile properties of rainbow smelt, Osmerus mordax, red muscle.
    Shuman JL
    Comparative biochemistry and physiology. Part A, Molecular and integrative physiology 196. (2016 Jun): 46-53.

    Contractile properties of the myotomal muscle of sheepshead, Archosargus probatocephalus.
    Akhtar M
    Journal of experimental zoology. Part A, Ecological genetics and physiology 323.3 (2015 Mar): 169-78.

    Unique expression of cytoskeletal proteins in human soft palate muscles.
    Stål P
    Journal of anatomy 228.3 (2016 Mar): 487-94.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Molecular analysis of fiber type-specific expression of murine myostatin promoter.
    Sharma M
    American journal of physiology. Cell physiology 287.4 (2004 Oct): C1031-40.

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells.
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation.
    Dunn SE
    FEBS letters 391.1-2 (1996 Aug 5): 39-44.

    Using laser capture microdissection to study fiber specific signaling in locomotor muscle in COPD: A pilot study.
    Polkey MI
    Muscle and nerve 55.6 (2017 Jun): 902-912.

    Rescue of Metabolic Alterations in AR113Q Skeletal Muscle by Peripheral Androgen Receptor Gene Silencing.
    Lieberman AP
    Cell reports 17.1 (2016 Sep 27): 125-136.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    WB References

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle and nerve 36.2 (2007 Aug): 183-9.

    Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.
    Vallejo-Illarramendi A
    Expert reviews in molecular medicine 18. (2016 Apr 8): e7.

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle and nerve 49.4 (2014 Apr): 534-44.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    IHC References

    Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy.
    Rüegg MA
    Skeletal muscle 3.1 (2013 Mar 6): 6.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Changes in a rat facial muscle after facial nerve injury and repair.
    Hildebrand C
    Muscle and nerve 24.9 (2001 Sep): 1202-12.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Muscle fiber pattern is independent of cell lineage in postnatal rodent development.
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply.
    Lindman R
    Journal of anatomy 197 ( Pt 2). (2000 Aug): 275-90.

    Morphological characterization of the levator veli palatini muscle in children born with cleft palates.
    Stål PS
    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association 38.5 (2001 Sep): 438-48.

    Abnormal palatopharyngeal muscle morphology in sleep-disordered breathing.
    Stål PS
    Journal of the neurological sciences 195.1 (2002 Mar 15): 11-23.

    Subcellular immunolocalisation of fatty acid translocase (FAT)/CD36 in human type-1 and type-2 skeletal muscle fibres.
    Luiken JJ
    Histochemistry and cell biology 121.2 (2004 Feb): 101-7.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle and nerve 36.2 (2007 Aug): 183-9.

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle and nerve 49.4 (2014 Apr): 534-44.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    ELISA References
    FFPE References
    Epitope Map References
    All References

    Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy.
    Rüegg MA
    Skeletal muscle 3.1 (2013 Mar 6): 6.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Changes in a rat facial muscle after facial nerve injury and repair.
    Hildebrand C
    Muscle and nerve 24.9 (2001 Sep): 1202-12.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Muscle fiber pattern is independent of cell lineage in postnatal rodent development.
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply.
    Lindman R
    Journal of anatomy 197 ( Pt 2). (2000 Aug): 275-90.

    Morphological characterization of the levator veli palatini muscle in children born with cleft palates.
    Stål PS
    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association 38.5 (2001 Sep): 438-48.

    Abnormal palatopharyngeal muscle morphology in sleep-disordered breathing.
    Stål PS
    Journal of the neurological sciences 195.1 (2002 Mar 15): 11-23.

    Subcellular immunolocalisation of fatty acid translocase (FAT)/CD36 in human type-1 and type-2 skeletal muscle fibres.
    Luiken JJ
    Histochemistry and cell biology 121.2 (2004 Feb): 101-7.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle and nerve 36.2 (2007 Aug): 183-9.

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle and nerve 49.4 (2014 Apr): 534-44.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Gene expression and fiber type variations in repeated vastus lateralis biopsies.
    Svensson MB
    Muscle and nerve 52.5 (2015 Nov): 812-7.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Length-dependent axo-terminal degeneration at the neuromuscular synapses of type II muscle in SOD1 mice.
    Farah MH
    Neuroscience 312. (2016 Jan 15): 179-89.

    Thermal acclimation to cold alters myosin content and contractile properties of rainbow smelt, Osmerus mordax, red muscle.
    Shuman JL
    Comparative biochemistry and physiology. Part A, Molecular and integrative physiology 196. (2016 Jun): 46-53.

    Contractile properties of the myotomal muscle of sheepshead, Archosargus probatocephalus.
    Akhtar M
    Journal of experimental zoology. Part A, Ecological genetics and physiology 323.3 (2015 Mar): 169-78.

    Unique expression of cytoskeletal proteins in human soft palate muscles.
    Stål P
    Journal of anatomy 228.3 (2016 Mar): 487-94.

    Molecular analysis of fiber type-specific expression of murine myostatin promoter.
    Sharma M
    American journal of physiology. Cell physiology 287.4 (2004 Oct): C1031-40.

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells.
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation.
    Dunn SE
    FEBS letters 391.1-2 (1996 Aug 5): 39-44.

    Using laser capture microdissection to study fiber specific signaling in locomotor muscle in COPD: A pilot study.
    Polkey MI
    Muscle and nerve 55.6 (2017 Jun): 902-912.

    Rescue of Metabolic Alterations in AR113Q Skeletal Muscle by Peripheral Androgen Receptor Gene Silencing.
    Lieberman AP
    Cell reports 17.1 (2016 Sep 27): 125-136.

    Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
    Kang PB
    Human molecular genetics 26.15 (2017 Aug 1): 2984-3000.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.
    Vallejo-Illarramendi A
    Expert reviews in molecular medicine 18. (2016 Apr 8): e7.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Reliable determination of training-induced alterations in muscle fiber composition in human skeletal muscle using quantitative polymerase chain reaction.
    Rønnestad BR
    Scandinavian journal of medicine and science in sports 24.5 (2014 Oct): e332-42.

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