M1-B4

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$40.00
SKU: M1-B4
View product citations for antibody M1-B4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Tenascin
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Chicken, Newt
Depositors Notes: The M1-B4 epitope is preserved is Zenker's fixative or freeze-substituted paraffin sections (see PMID: 2464073). Historically, this antigen has been known as myotendinous antigen, neuronectin, glioma-associated ECM antigen and cytotactin.
Antigen Molecular Weight: 150-240 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: TNC
Immunogen: Pepsin digest of type V collagen
Alternate Gene Names: GMEM, JI, TN, TN-C
Alternate Antibody Name: tenascin
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P10039 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396440 
Additional Characterization:
Antibody Registry ID: AB_528488 
Additional Information: RRID: AB_528488
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M1-B4 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product M1-B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis.
    Fambrough DM
    The Journal of cell biology 98.6 (1984 Jun): 1926-36.

    The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth.
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    WB References
    IHC References

    Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis.
    Fambrough DM
    The Journal of cell biology 98.6 (1984 Jun): 1926-36.

    Tenascin: an extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis.
    Sakakura T
    Cell 47.1 (1986 Oct 10): 131-9.

    J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo.
    Schachner M
    Development (Cambridge, England) 107.2 (1989 Oct): 309-19.

    Enhanced expression of neural cell adhesion molecules and tenascin (cytotactin) during wound healing.
    Chen HM
    The American journal of pathology 138.2 (1991 Feb): 427-40.

    Computer-aided mechanogenesis of skeletal muscle organs from single cells in vitro.
    Karlisch P
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 5.13 (1991 Oct): 2860-7.

    Adhesion molecules in skin development: morphogenesis of feather and hair.
    Chia J
    Annals of the New York Academy of Sciences 642. (1991 Dec 26): 263-80.

    Mechanism of skin morphogenesis. I. Analyses with antibodies to adhesion molecules tenascin, N-CAM, and integrin.
    Chuong CM
    Developmental biology 150.1 (1992 Mar): 82-98.

    Basal lamina development in chicken muscle spindles.
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 202.3 (1995 Mar): 284-93.

    IP References
    ELISA References
    All References

    Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis.
    Fambrough DM
    The Journal of cell biology 98.6 (1984 Jun): 1926-36.

    Tenascin: an extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis.
    Sakakura T
    Cell 47.1 (1986 Oct 10): 131-9.

    J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo.
    Schachner M
    Development (Cambridge, England) 107.2 (1989 Oct): 309-19.

    Enhanced expression of neural cell adhesion molecules and tenascin (cytotactin) during wound healing.
    Chen HM
    The American journal of pathology 138.2 (1991 Feb): 427-40.

    Computer-aided mechanogenesis of skeletal muscle organs from single cells in vitro.
    Karlisch P
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 5.13 (1991 Oct): 2860-7.

    Adhesion molecules in skin development: morphogenesis of feather and hair.
    Chia J
    Annals of the New York Academy of Sciences 642. (1991 Dec 26): 263-80.

    Mechanism of skin morphogenesis. I. Analyses with antibodies to adhesion molecules tenascin, N-CAM, and integrin.
    Chuong CM
    Developmental biology 150.1 (1992 Mar): 82-98.

    Basal lamina development in chicken muscle spindles.
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 202.3 (1995 Mar): 284-93.

    The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth.
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    A major, six-armed glycoprotein from embryonic cartilage.
    Winterhalter KH
    The EMBO journal 6.2 (1987 Feb): 349-53.

    Chick myotendinous antigen. II. A novel extracellular glycoprotein complex consisting of large disulfide-linked subunits.
    Fambrough DM
    The Journal of cell biology 98.6 (1984 Jun): 1937-46.

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