AMV-3C2

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$40.00
SKU: AMV-3C2
View product citations for antibody AMV-3C2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: avian myoblastosis virus, p19^(gag)
Hybridoma Cells Available: Yes
Antigen Species: Avian myoblastosis virus (AMV)
Depositor: Boettiger, D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pennsylvania
Positive Tested Species Reactivity: Avian myoblastosis virus (AMV)
Depositors Notes: This antibody identifies cells infected with all strains of the avian sarcoma/leukemia virus complex and can also be used to identify cells harboring Rous sarcoma or avian leukemia virus-derived vectors. Gag-Pro polyprotein is cleaved into protein fragments; p19 represents the first 155 amino acids of the Gag-Pro polyprotein.
Antigen Molecular Weight: Predicted: 16.5kDa; Apparent: 19kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: gag-pro
Immunogen: Avian Myeloblastosis Virus purified from infected chickens.
Alternate Gene Names:
Alternate Antibody Name: 3C2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: N-terminal a.a.1-51 near a.a. 40.
Uniprot ID: P03322 
Immunogen Sequence: Full length protein
Entrez Gene ID: 1491923 
Additional Characterization:
Antibody Registry ID: AB_528098 
Additional Information: Abdominal-b has two isoforms, M and R; isoform R lacks the amino terminal 223 aa portion of isoform M. Both M and R isoforms are recognized by mAb 1A2E9.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AMV-3C2 was deposited to the DSHB by Boettiger, D. (DSHB Hybridoma Product AMV-3C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

43 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of general virology
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    IF References

    Developmental biology
    Shukunami C
    Developmental biology 332.2 (2009 Aug 15): 196-211.

    Developmental biology
    Adler R
    Developmental biology 298.1 (2006 Oct 1): 272-84.

    BMC developmental biology
    Canto-Soler MV
    BMC developmental biology 10. (2010 Oct 18): 106.

    Developmental biology
    Fekete DM
    Developmental biology 352.1 (2011 Apr 1): 27-39.

    PloS one
    Del Rio-Tsonis K
    PloS one 9.7 (2014): e101748.

    Proceedings of the National Academy of Sciences of the United States of America
    Chuong CM
    Proceedings of the National Academy of Sciences of the United States of America 110.16 (2013 Apr 16): E1452-61.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Monnier PP
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.4 (2009 Jan 28): 1126-31.

    Mechanisms of development
    Mansfield JH
    Mechanisms of development 130.4-5 (2013 Apr-May): 226-40.

    Cell death and differentiation
    Yaginuma H
    Cell death and differentiation 13.3 (2006 Mar): 435-45.

    Development (Cambridge, England)
    Del Rio-Tsonis K
    Development (Cambridge, England) 131.18 (2004 Sep): 4607-21.

    Development (Cambridge, England)
    Tabin CJ
    Development (Cambridge, England) 131.16 (2004 Aug): 3885-96.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 229.3 (2004 Mar): 555-63.

    Neuron
    Jessell TM
    Neuron 32.6 (2001 Dec 20): 997-1012.

    Developmental biology
    Hudspeth AJ
    Developmental biology 354.2 (2011 Jun 15): 221-31.

    WB References

    The Journal of general virology
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    Journal of virology
    Schambach A
    Journal of virology 84.13 (2010 Jul): 6626-35.

    IHC References

    Developmental biology
    Mina M
    Developmental biology 316.2 (2008 Apr 15): 336-49.

    Developmental biology
    Wada N
    Developmental biology 342.2 (2010 Jun 15): 157-68.

    Molecular immunology
    Kaspers B
    Molecular immunology 47.7-8 (2010 Apr): 1619-28.

    PloS one
    Renshaw PF
    PloS one 9.8 (2014): e104937.

    PLoS genetics
    Chuong CM
    PLoS genetics 8.7 (2012): e1002748.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Neubüser A
    Developmental dynamics : an official publication of the American Association of Anatomists 239.11 (2010 Nov): 2860-74.

    Mechanisms of development
    Cairine Logan C
    Mechanisms of development 122.7-8 (2005 Jul): 900-13.

    Developmental biology
    Klewer SE
    Developmental biology 278.1 (2005 Feb 1): 35-48.

    Developmental biology
    Marx M
    Developmental biology 283.2 (2005 Jul 15): 335-44.

    Proceedings of the National Academy of Sciences of the United States of America
    Prum RO
    Proceedings of the National Academy of Sciences of the United States of America 102.33 (2005 Aug 16): 11734-9.

    Developmental biology
    Chuong CM
    Developmental biology 266.1 (2004 Feb 1): 109-22.

    BMC developmental biology
    Cepko CL
    BMC developmental biology 6. (2006 Jan 22): 2.

    FFPE References

    Genome biology and evolution
    Li WH
    Genome biology and evolution 6.9 (2014 Aug 24): 2258-73.

    PLoS genetics
    Chuong CM
    PLoS genetics 8.7 (2012): e1002748.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Neubüser A
    Developmental dynamics : an official publication of the American Association of Anatomists 239.11 (2010 Nov): 2860-74.

    Epitope Map References

    The Journal of general virology
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    All References

    Developmental biology
    Mina M
    Developmental biology 316.2 (2008 Apr 15): 336-49.

    Developmental biology
    Wada N
    Developmental biology 342.2 (2010 Jun 15): 157-68.

    Molecular immunology
    Kaspers B
    Molecular immunology 47.7-8 (2010 Apr): 1619-28.

    PloS one
    Renshaw PF
    PloS one 9.8 (2014): e104937.

    PLoS genetics
    Chuong CM
    PLoS genetics 8.7 (2012): e1002748.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Neubüser A
    Developmental dynamics : an official publication of the American Association of Anatomists 239.11 (2010 Nov): 2860-74.

    Mechanisms of development
    Cairine Logan C
    Mechanisms of development 122.7-8 (2005 Jul): 900-13.

    Developmental biology
    Klewer SE
    Developmental biology 278.1 (2005 Feb 1): 35-48.

    Developmental biology
    Marx M
    Developmental biology 283.2 (2005 Jul 15): 335-44.

    Proceedings of the National Academy of Sciences of the United States of America
    Prum RO
    Proceedings of the National Academy of Sciences of the United States of America 102.33 (2005 Aug 16): 11734-9.

    Developmental biology
    Chuong CM
    Developmental biology 266.1 (2004 Feb 1): 109-22.

    BMC developmental biology
    Cepko CL
    BMC developmental biology 6. (2006 Jan 22): 2.

    Developmental biology
    Shukunami C
    Developmental biology 332.2 (2009 Aug 15): 196-211.

    Developmental biology
    Adler R
    Developmental biology 298.1 (2006 Oct 1): 272-84.

    BMC developmental biology
    Canto-Soler MV
    BMC developmental biology 10. (2010 Oct 18): 106.

    Developmental biology
    Fekete DM
    Developmental biology 352.1 (2011 Apr 1): 27-39.

    PloS one
    Del Rio-Tsonis K
    PloS one 9.7 (2014): e101748.

    Proceedings of the National Academy of Sciences of the United States of America
    Chuong CM
    Proceedings of the National Academy of Sciences of the United States of America 110.16 (2013 Apr 16): E1452-61.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Monnier PP
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.4 (2009 Jan 28): 1126-31.

    Mechanisms of development
    Mansfield JH
    Mechanisms of development 130.4-5 (2013 Apr-May): 226-40.

    Cell death and differentiation
    Yaginuma H
    Cell death and differentiation 13.3 (2006 Mar): 435-45.

    Development (Cambridge, England)
    Del Rio-Tsonis K
    Development (Cambridge, England) 131.18 (2004 Sep): 4607-21.

    Development (Cambridge, England)
    Tabin CJ
    Development (Cambridge, England) 131.16 (2004 Aug): 3885-96.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 229.3 (2004 Mar): 555-63.

    Neuron
    Jessell TM
    Neuron 32.6 (2001 Dec 20): 997-1012.

    Developmental biology
    Hudspeth AJ
    Developmental biology 354.2 (2011 Jun 15): 221-31.

    The Journal of general virology
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    Journal of virology
    Schambach A
    Journal of virology 84.13 (2010 Jul): 6626-35.

    Genome biology and evolution
    Li WH
    Genome biology and evolution 6.9 (2014 Aug 24): 2258-73.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Nishi R
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.43 (2007 Oct 24): 11501-9.

    Neuroscience letters
    Zimmermann H
    Neuroscience letters 485.3 (2010 Nov 26): 208-11.

    Journal of neurochemistry
    Kim KS
    Journal of neurochemistry 98.3 (2006 Aug): 773-81.

    Developmental biology
    Laufer E
    Developmental biology 347.1 (2010 Nov 1): 133-46.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Chédotal A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.22 (2006 May 31): 6082-8.

    Developmental biology
    Lichtler AC
    Developmental biology 316.2 (2008 Apr 15): 458-70.

    The Journal of biological chemistry
    Kim KS
    The Journal of biological chemistry 283.24 (2008 Jun 13): 16860-7.

    Virology
    Linial ML
    Virology 347.1 (2006 Mar 30): 226-33.

    Proceedings of the National Academy of Sciences of the United States of America
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Mechanisms of development
    Ryan AK
    Mechanisms of development 123.3 (2006 Mar): 210-27.

    Developmental biology
    Brand-Saberi B
    Developmental biology 277.1 (2005 Jan 1): 42-50.

    Developmental biology
    Clegg DO
    Developmental biology 276.2 (2004 Dec 15): 416-30.

    Developmental cell
    McMahon AP
    Developmental cell 16.4 (2009 Apr): 600-6.

    Developmental cell
    Monnier PP
    Developmental cell 22.2 (2012 Feb 14): 391-402.

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