3B5

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  • Immunofluorescent image of a cryosection of a chicken embryo cephalic region labeled with DSHB antibody 3B5 against AP-2 alpha. Image Credit: Cathy Danesin (Inserm) and Eric Theveneau (CNRS).
  • Fluorescent microscope image of an embryonic mouse section through the fourth pharyngeal arch artery. Ectoderm and neural crest cells are labeled with DSHB antibody 3B5 (red). PMID: 15996652, Fig. 5E
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SKU: 3B5

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Catalog Fields

Clone ID/Product Name: 3B5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TFAP2A
Ab Isotype: MIgG2b, kappa light chain
Gene Name:
Antibody Registry ID: AB_528084 
Uniprot ID: P05549 
RRID:  
Entrez Gene ID: 7020 
Clonality: Monoclonal
Immunogen: AP-2 alpha delta N165 (DNA-binding domain)
Clone:
Immunogen Sequence: a.a. 166-437
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: AP-2 alpha
Epitope Location or Sequence: AP-2 alpha sequence (amino acids 166-197)/ GINIPDQTVIKKGPVSLSKSNSNAVSAIPINK
Alternate Antigen Name:
Deposit Date: 3/24/1999
Antigen Molecular Weight: Predicted: 48kDa; Apparent: 50kDa
Depositor: Williams, T.J.
Antigen Sequence:
Depositor Institution: Yale University
Antigen Species: Human
Depositor Notes: AP-2 alpha specific. Does not react with AP-2 beta or AP-2 gamma. Immunoprecipitation also EMSA supershift analysis and ChIP-seq. Immunohistochemistry requires antigen retrieval, {see PMID: 9850080}.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chicken, Human, Mouse, Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
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