Clone ID / Name: 3B5
Anti - AP-2 alpha
Immunofluorescent image of a cryosection of a chicken embryo cephalic region labeled with DSHB antibody 3B5 against AP-2 alpha. Image Credit: Cathy Danesin (Inserm) and Eric Theveneau (CNRS).
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: MIgG2b, kappa light chain
Myeloma Strain: SP2/0
Antibody Registry / RRID: AB_528084
Available to For-Profits: Yes
Hybridoma Available: No
Immunogen & Epitope
Immunogen: AP-2 alpha delta N165 (DNA-binding domain)
Immunogen Sequence: a.a. 166-437
Epitope Mapped: Yes
Epitope Location / Seq: AP-2 alpha sequence (amino acids 166-197)/ GINIPDQTVIKKGPVSLSKSNSNAVSAIPINK
Clone: —
Additional Info: —
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Chicken, Human, Mouse, Zebrafish
Predicted Reactivity: —
Depositor
Depositor Name: T.J. Williams
Institution: Yale University
Deposit Date: March 24, 1999
Depositor Notes: AP-2 alpha specific. Does not react with AP-2 beta or AP-2 gamma. Immunoprecipitation also EMSA supershift analysis and ChIP-seq. Immunohistochemistry requires antigen retrieval, {see PMID: 9850080}.
Recommended Applications
ChIP
FFPE
Gel Supershift
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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