BF-G6
Catalog Fields
Antigen: Myosin heavy chain, embryonic
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padova
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: BF-G6 antibody recognizes embryonic myosin heavy chain on fresh frozen or acetone-fixed cultures of rhabdomyosarcomas and Wilms' tumors.
Predicted Species Reactivity:
Gene: MYH3
Immunogen: Purified myosin from embryonic bovine skeletal muscle
Alternate Gene Names: HEMHC, MYHC-EMB, MYHSE1, SMHCE
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-0
Epitope Location or Sequence:
Uniprot ID: P11055
Immunogen Sequence: Full length protein
Entrez Gene ID: 4621
Antibody Registry ID: AB_10571455
Additional Information: RRID:AB_10571455
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-G6 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-G6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study.
Carli M
Experimental cell research 163.1 (1986 Mar): 211-20.