BF-G6

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$40.00
SKU: BF-G6
View product citations for antibody BF-G6 on CiteAb

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Available: 99

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain, embryonic
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padov
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: BF-G6 antibody recognizes embryonic myosin heavy chain on fresh frozen or acetone-fixed cultures of rhabdomyosarcomas and Wilms' tumors.
Antigen Molecular Weight: 223 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MYH3
Immunogen: Purified myosin from embryonic bovine skeletal muscle
Alternate Gene Names: HEMHC, MYHC-EMB, MYHSE1, SMHCE
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-0
Epitope Location or Sequence:
Uniprot ID: P11055 
Immunogen Sequence: Full length protein
Entrez Gene ID: 4621 
Additional Characterization:  
Antibody Registry ID: AB_10571455 
Additional Information: RRID:AB_10571455
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-G6 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-G6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study.
    Carli M
    Experimental cell research 163.1 (1986 Mar): 211-20.

    Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle.
    Lømo T
    Developmental biology 127.1 (1988 May): 1-11.

    MicroRNA deep sequencing in two adult stem cell populations identifies miR-501 as a novel regulator of myosin heavy chain during muscle regeneration.
    Krützfeldt J
    Development (Cambridge, England) 143.22 (2016 Nov 15): 4137-4148.

    MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity.
    Schiaffino S
    Nature communications 7. (2016 Aug 3): 12397.

    Egr3-dependent muscle spindle stretch receptor intrafusal muscle fiber differentiation and fusimotor innervation homeostasis.
    Tourtellotte WG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5566-78.

    Myofiber-specific inhibition of TGFβ signaling protects skeletal muscle from injury and dystrophic disease in mice.
    Molkentin JD
    Human molecular genetics 23.25 (2014 Dec 20): 6903-15.

    Local overexpression of V1a-vasopressin receptor enhances regeneration in tumor necrosis factor-induced muscle atrophy.
    Scicchitano BM
    BioMed research international 2014. (2014): 235426.

    Participation of myosin Va and Pka type I in the regeneration of neuromuscular junctions.
    Rudolf R
    PloS one 7.7 (2012): e40860.

    Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles.
    Schiaffino S
    The Journal of physiology 588.Pt 2 (2010 Jan 15): 353-64.

    WB References
    IHC References
    ELISA References
    All References

    Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study.
    Carli M
    Experimental cell research 163.1 (1986 Mar): 211-20.

    Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle.
    Lømo T
    Developmental biology 127.1 (1988 May): 1-11.

    Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice.
    Molkentin JD
    Human molecular genetics 25.6 (2016 Mar 15): 1192-202.

    Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle.
    Reggiani C
    The Journal of physiology 437. (1991 Jun): 655-72.

    An embryonic-like myosin heavy chain is transiently expressed in nodal conduction tissue of the rat heart.
    Ausoni S
    Journal of molecular and cellular cardiology 20.10 (1988 Oct): 931-41.

    MicroRNA deep sequencing in two adult stem cell populations identifies miR-501 as a novel regulator of myosin heavy chain during muscle regeneration.
    Krützfeldt J
    Development (Cambridge, England) 143.22 (2016 Nov 15): 4137-4148.

    MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity.
    Schiaffino S
    Nature communications 7. (2016 Aug 3): 12397.

    Egr3-dependent muscle spindle stretch receptor intrafusal muscle fiber differentiation and fusimotor innervation homeostasis.
    Tourtellotte WG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5566-78.

    Myofiber-specific inhibition of TGFβ signaling protects skeletal muscle from injury and dystrophic disease in mice.
    Molkentin JD
    Human molecular genetics 23.25 (2014 Dec 20): 6903-15.

    Local overexpression of V1a-vasopressin receptor enhances regeneration in tumor necrosis factor-induced muscle atrophy.
    Scicchitano BM
    BioMed research international 2014. (2014): 235426.

    Participation of myosin Va and Pka type I in the regeneration of neuromuscular junctions.
    Rudolf R
    PloS one 7.7 (2012): e40860.

    Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles.
    Schiaffino S
    The Journal of physiology 588.Pt 2 (2010 Jan 15): 353-64.

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