BF-45

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$40.00
SKU: BF-45
View product citations for antibody BF-45 on CiteAb

In Stock

Available: 117

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain, embryonic/ myosin-3
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padov
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: BF-G6 antibody recognizes embryonic myosin heavy chain on fresh frozen or acetone-fixed cultures of rhabdomyosarcomas and Wilms' tumors.
Antigen Molecular Weight: 223 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MYH3
Immunogen: Purified myosin from embryonic bovine skeletal muscle
Alternate Gene Names: HEMHC, MYHC-EMB, MYHSE1, SMHCE
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-0
Epitope Location or Sequence:
Uniprot ID: P11055 
Immunogen Sequence: Full length protein
Entrez Gene ID: 4621 
Additional Characterization:  
Antibody Registry ID: AB_10573810 
Additional Information: RRID:AB_10573810
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-45 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-45)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Molecular signals governing cremaster muscle development: clues for cryptorchidism.
    Hutson JM
    Journal of pediatric surgery 49.2 (2014 Feb): 312-6; discussion 316.

    WB References
    IHC References

    Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle.
    Lømo T
    Developmental biology 127.1 (1988 May): 1-11.

    Effects of chronic AICAR administration on the metabolic and contractile phenotypes of rat slow- and fast-twitch skeletal muscles.
    Putman CT
    Canadian journal of physiology and pharmacology 81.11 (2003 Nov): 1072-82.

    A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells.
    Miller JB
    Development (Cambridge, England) 117.3 (1993 Mar): 1125-33.

    Laminin-111 improves skeletal muscle stem cell quantity and function following eccentric exercise.
    Boppart MD
    Stem cells translational medicine 3.9 (2014 Sep): 1013-22.

    Effects of chronic AICAR administration on the metabolic and contractile phenotypes of rat slow- and fast-twitch skeletal muscles.
    Putman CT
    Canadian journal of physiology and pharmacology 81.11 (2003 Nov): 1072-82.

    Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study.
    Carli M
    Experimental cell research 163.1 (1986 Mar): 211-20.

    Localization of T-cell factor 4 positive fibroblasts and CD206-positive macrophages during skeletal muscle regeneration in mice.
    Abe S
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 235. (2021 May): 151694.

    Localization of T-cell factor 4 positive fibroblasts and CD206-positive macrophages during skeletal muscle regeneration in mice.
    Abe S
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 235. (2021 May): 151694.

    IP References
    All References

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