ChAT4B1
Catalog Fields
Antigen: choline acetyltransferase
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Salvaterra, P.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Manduca sexta
Depositors Institution: City of Hope, Beckman Research Institute
Antigen Molecular Weight: 81 kDa; cleaved into three chains of 13, 54, and 67 kDa
Depositors Notes:
Immunogen: Recombinant fusion protein
Gene: cha
Alternate Antibody Name: 4B1
Alternate Gene Names: CG12345, ChAT
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: No
Uniprot ID: P07668
Epitope Location or Sequence:
Entrez Gene ID: 42249
Immunogen Sequence: Total protein
Antibody Registry ID: AB_528122
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: Choline O-acetyltransferase catalyzes the reversible synthesis of acetylcholine from acetyl CoA and choline at cholinergic synapses.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ChAT4B1 was deposited to the DSHB by Salvaterra, P.M. (DSHB Hybridoma Product ChAT4B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
23 References
Initial Publication
Analysis of choline acetyltransferase protein in temperature sensitive mutant flies using newly generated monoclonal antibody.
Salvaterra P
Neuroscience research 24.3 (1996 Feb): 237-43.