ChAT4B1

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$40.00
SKU: ChAT4B1
View product citations for antibody ChAT4B1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: choline acetyltransferase
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Salvaterra, P.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Beckman Research Institute, City of Hope
Positive Tested Species Reactivity: Drosophila, Manduca sexta
Depositors Notes:
Antigen Molecular Weight: 81 kDa; cleaved into three chains of 13, 54, and 67 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cha
Immunogen: Recombinant fusion protein
Alternate Gene Names: CG12345, ChAT
Alternate Antibody Name: 4B1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID: P07668 
Immunogen Sequence: Total protein
Entrez Gene ID: 42249 
Additional Characterization:  
Antibody Registry ID: AB_528122 
Additional Information: Choline O-acetyltransferase catalyzes the reversible synthesis of acetylcholine from acetyl CoA and choline at cholinergic synapses.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ChAT4B1 was deposited to the DSHB by Salvaterra, P.M. (DSHB Hybridoma Product ChAT4B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    Neurotransmitter-induced changes in the intracellular calcium concentration suggest a differential central modulation of CCAP neuron subsets in Drosophila.
    Wegener C
    Developmental neurobiology 67.6 (2007 May): 792-808.

    Physiological and morphological characterization of local interneurons in the Drosophila antennal lobe.
    Hansson BS
    Journal of neurophysiology 104.2 (2010 Aug): 1007-19.

    Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis.
    King SM
    The Journal of cell biology 146.1 (1999 Jul 12): 165-80.

    Excitatory local circuits and their implications for olfactory processing in the fly antennal lobe.
    Miesenböck G
    Cell 128.3 (2007 Feb 9): 601-12.

    Neurons with cholinergic phenotype in the visual system of Drosophila.
    Borst A
    The Journal of comparative neurology 519.1 (2011 Jan 1): 162-76.

    Neuroblast lineage-specific origin of the neurons of the Drosophila larval olfactory system.
    Hartenstein V
    Developmental biology 373.2 (2013 Jan 15): 322-37.

    Expression of muscarinic acetylcholine receptors and choline acetyltransferase enzyme in cultured antennal sensory neurons and non-neural cells of the developing moth Manduca sexta.
    Meisner S
    Journal of neurobiology 62.3 (2005 Feb 15): 316-29.

    Glutamate, GABA and acetylcholine signaling components in the lamina of the Drosophila visual system.
    Nässel DR
    PloS one 3.5 (2008 May 7): e2110.

    Different classes of input and output neurons reveal new features in microglomeruli of the adult Drosophila mushroom body calyx.
    Meinertzhagen IA
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2185-201.

    Brain aging, memory impairment and oxidative stress: a study in Drosophila melanogaster.
    Ramesh SR
    Behavioural brain research 259. (2014 Feb 1): 60-9.

    Immunoreactivity against choline acetyltransferase, gamma-aminobutyric acid, histamine, octopamine, and serotonin in the larval chemosensory system of Dosophila melanogaster.
    Stocker RF
    The Journal of comparative neurology 453.2 (2002 Nov 11): 157-67.

    Characterization of the octopaminergic and tyraminergic neurons in the central brain of Drosophila larvae.
    Thum AS
    The Journal of comparative neurology 522.15 (2014 Oct 15): 3485-500.

    Colocalization of allatotropin and tachykinin-related peptides with classical transmitters in physiologically distinct subtypes of olfactory local interneurons in the cockroach (Periplaneta americana).
    Kloppenburg P
    The Journal of comparative neurology 523.10 (2015 Jul 1): 1569-86.

    Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae.
    Thum AS
    The Journal of comparative neurology 523.18 (2015 Dec 15): 2637-64.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

    WB References
    IHC References
    IP References
    ELISA References
    FFPE References

    Synaptic organization of the mushroom body calyx in Drosophila melanogaster.
    Schürmann FW
    The Journal of comparative neurology 445.3 (2002 Apr 8): 211-26.

    All References

    Localization of choline acetyltransferase-expressing neurons in the larval visual system of Drosophila melanogaster.
    Salvaterra PM
    Cell and tissue research 282.2 (1995 Nov): 193-202.

    Immunocytochemical localization of choline acetyltransferase and muscarinic ACh receptors in the antenna during development of the sphinx moth Manduca sexta.
    Torkkeli PH
    Cell and tissue research 320.1 (2005 Apr): 163-73.

    Synaptic organization of the mushroom body calyx in Drosophila melanogaster.
    Schürmann FW
    The Journal of comparative neurology 445.3 (2002 Apr 8): 211-26.

    RNA-Seq Transcriptome Analysis of Direction-Selective T4/T5 Neurons in Drosophila.
    Borst A
    PloS one 11.9 (2016): e0163986.

    Serotonin transporter dependent modulation of food-seeking behavior.
    Scholz H
    PloS one 15.1 (2020): e0227554.

    Neurotransmitter-induced changes in the intracellular calcium concentration suggest a differential central modulation of CCAP neuron subsets in Drosophila.
    Wegener C
    Developmental neurobiology 67.6 (2007 May): 792-808.

    Physiological and morphological characterization of local interneurons in the Drosophila antennal lobe.
    Hansson BS
    Journal of neurophysiology 104.2 (2010 Aug): 1007-19.

    Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis.
    King SM
    The Journal of cell biology 146.1 (1999 Jul 12): 165-80.

    Excitatory local circuits and their implications for olfactory processing in the fly antennal lobe.
    Miesenböck G
    Cell 128.3 (2007 Feb 9): 601-12.

    Neurons with cholinergic phenotype in the visual system of Drosophila.
    Borst A
    The Journal of comparative neurology 519.1 (2011 Jan 1): 162-76.

    Neuroblast lineage-specific origin of the neurons of the Drosophila larval olfactory system.
    Hartenstein V
    Developmental biology 373.2 (2013 Jan 15): 322-37.

    Expression of muscarinic acetylcholine receptors and choline acetyltransferase enzyme in cultured antennal sensory neurons and non-neural cells of the developing moth Manduca sexta.
    Meisner S
    Journal of neurobiology 62.3 (2005 Feb 15): 316-29.

    Glutamate, GABA and acetylcholine signaling components in the lamina of the Drosophila visual system.
    Nässel DR
    PloS one 3.5 (2008 May 7): e2110.

    Different classes of input and output neurons reveal new features in microglomeruli of the adult Drosophila mushroom body calyx.
    Meinertzhagen IA
    The Journal of comparative neurology 520.10 (2012 Jul 1): 2185-201.

    Brain aging, memory impairment and oxidative stress: a study in Drosophila melanogaster.
    Ramesh SR
    Behavioural brain research 259. (2014 Feb 1): 60-9.

    Immunoreactivity against choline acetyltransferase, gamma-aminobutyric acid, histamine, octopamine, and serotonin in the larval chemosensory system of Dosophila melanogaster.
    Stocker RF
    The Journal of comparative neurology 453.2 (2002 Nov 11): 157-67.

    Characterization of the octopaminergic and tyraminergic neurons in the central brain of Drosophila larvae.
    Thum AS
    The Journal of comparative neurology 522.15 (2014 Oct 15): 3485-500.

    Colocalization of allatotropin and tachykinin-related peptides with classical transmitters in physiologically distinct subtypes of olfactory local interneurons in the cockroach (Periplaneta americana).
    Kloppenburg P
    The Journal of comparative neurology 523.10 (2015 Jul 1): 1569-86.

    Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae.
    Thum AS
    The Journal of comparative neurology 523.18 (2015 Dec 15): 2637-64.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

    Analysis of choline acetyltransferase protein in temperature sensitive mutant flies using newly generated monoclonal antibody.
    Salvaterra P
    Neuroscience research 24.3 (1996 Feb): 237-43.

    Abnormal chemosensory jump 6 is a positive transcriptional regulator of the cholinergic gene locus in Drosophila olfactory neurons.
    Salvaterra PM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.13 (2002 Jul 1): 5291-9.

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