3H2 2D7

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$40.00
SKU: 3H2 2D7
View product citations for antibody 3H2 2D7 on CiteAb

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Available: 242

DSHB Data Sheet

Catalog Fields

Antigen: Synaptotagmin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Crayfish, Drosophila, Insect, Mouse
Depositors Notes: Insect species that tested positive include Manduca sexta (tobacco hornworm), Periplaneta americana (cockroach), Orthoptera sp. (locust), and Bombyx mori (silkworm moth) Crustaceans include the freshwater crayfish.
Antigen Molecular Weight: Predicted: multiple isoforms at 19, 37, and 53kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Syt1
Immunogen: Synaptotagmin recombinant, purified.
Alternate Gene Names: Droso1; DSypt; dSYT; DSYT; dsyt1; Dsyt2; SYT1;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence:
Uniprot ID: P21521 
Immunogen Sequence: Recombinant synaptotagmin, Partial protein (= conserved cytoplasmic domain, amino acids 134-474)
Entrez Gene ID: 33473 
Additional Characterization:
Antibody Registry ID: AB_528483 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H2 2D7 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 3H2 2D7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
    Tolbert LP
    The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.

    IF References

    Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
    Tolbert LP
    The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.

    Drosophila Fip200 is an essential regulator of autophagy that attenuates both growth and aging.
    Lee JH
    Autophagy 9.8 (2013 Aug): 1201-13.

    Modular subdivision of mushroom bodies by Kenyon cells in the silkmoth.
    Kanzaki R
    The Journal of comparative neurology 513.3 (2009 Mar 20): 315-30.

    Development of a scheme and tools to construct a standard moth brain for neural network simulations.
    Kanzaki R
    Computational intelligence and neuroscience 2012. (2012): 795291.

    Neurogenesis in the circumventricular organs of adult mouse brains.
    Miyata S
    Journal of neuroscience research 91.6 (2013 Jun): 757-70.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    The translational repressor Pumilio regulates presynaptic morphology and controls postsynaptic accumulation of translation factor eIF-4E.
    Zinn K
    Neuron 44.4 (2004 Nov 18): 663-76.

    Negative regulation of active zone assembly by a newly identified SR protein kinase.
    Davis GW
    PLoS biology 7.9 (2009 Sep): e1000193.

    Abnormal synaptic vesicle biogenesis in Drosophila synaptogyrin mutants.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.50 (2012 Dec 12): 18054-67, 18067a.

    WB References

    Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
    Tolbert LP
    The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.

    IHC References

    Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
    Tolbert LP
    The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.

    Epitope Map References

    Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
    Tolbert LP
    The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.

    All References

    Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
    Tolbert LP
    The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.

    Drosophila Fip200 is an essential regulator of autophagy that attenuates both growth and aging.
    Lee JH
    Autophagy 9.8 (2013 Aug): 1201-13.

    Modular subdivision of mushroom bodies by Kenyon cells in the silkmoth.
    Kanzaki R
    The Journal of comparative neurology 513.3 (2009 Mar 20): 315-30.

    Development of a scheme and tools to construct a standard moth brain for neural network simulations.
    Kanzaki R
    Computational intelligence and neuroscience 2012. (2012): 795291.

    Neurogenesis in the circumventricular organs of adult mouse brains.
    Miyata S
    Journal of neuroscience research 91.6 (2013 Jun): 757-70.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    The translational repressor Pumilio regulates presynaptic morphology and controls postsynaptic accumulation of translation factor eIF-4E.
    Zinn K
    Neuron 44.4 (2004 Nov 18): 663-76.

    Negative regulation of active zone assembly by a newly identified SR protein kinase.
    Davis GW
    PLoS biology 7.9 (2009 Sep): e1000193.

    Abnormal synaptic vesicle biogenesis in Drosophila synaptogyrin mutants.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.50 (2012 Dec 12): 18054-67, 18067a.

    Confocal imaging of fluorescently labeled proteins in the Drosophila larval neuromuscular junction.
    Coyle IP
    Methods in molecular biology (Clifton, N.J.) 1075. (2014): 201-12.

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