3H2 2D7
Catalog Fields
Antigen: Synaptotagmin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Crayfish, Drosophila, Insect, Mouse
Depositors Notes: Insect species that tested positive include Manduca sexta (tobacco hornworm), Periplaneta americana (cockroach), Orthoptera sp. (locust), and Bombyx mori (silkworm moth) Crustaceans include the freshwater crayfish.
Predicted Species Reactivity:
Gene: Syt1
Immunogen: Synaptotagmin recombinant, purified.
Alternate Gene Names: Droso1; DSypt; dSYT; DSYT; dsyt1; Dsyt2; SYT1;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence:
Uniprot ID: P21521
Immunogen Sequence: Recombinant synaptotagmin, Partial protein (= conserved cytoplasmic domain, amino acids 134-474)
Entrez Gene ID: 33473
Antibody Registry ID: AB_528483
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H2 2D7 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 3H2 2D7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
Tolbert LP
The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.