3H2 2D7
Catalog Fields
Clone ID/Product Name: 3H2 2D7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Syt1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 33473
Clonality: Monoclonal
Immunogen: Synaptotagmin recombinant, purified.
Clone:
Immunogen Sequence: Recombinant synaptotagmin, Partial protein (= conserved cytoplasmic domain, amino acids 134-474)
Myeloma Strain: HL-1
Epitope Mapped: Yes
Antigen Name: Synaptotagmin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/16/2004
Antigen Molecular Weight: Predicted: multiple isoforms at 19, 37, and 53kDa
Depositor: Zinn, K.
Antigen Sequence:
Depositor Institution: California Institute of Technology
Antigen Species: Drosophila
Depositor Notes: Insect species that tested positive include Manduca sexta (tobacco hornworm), Periplaneta americana (cockroach), Orthoptera sp. (locust), and Bombyx mori (silkworm moth) Crustaceans include the freshwater crayfish.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Crayfish, Drosophila, Insect, Mouse
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H2 2D7 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 3H2 2D7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta.
Tolbert LP
The Journal of comparative neurology 441.4 (2001 Dec 24): 277-87.