Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S. / Colley, N.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila, Insect, Mosquito, Planaria
Depositors Notes: Blots well using 4% SDS-PAGE
Antigen Molecular Weight: Predicted: 575 kDa, Apparent: >500kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: adult fly heads homogenates
Alternate Gene Names: 22C10/FUTSCH; 22C20; CG14772; CG3064; CG34387; Dmel\CG34387; EG:49E4.1; Futch; Futsch; lincRNA.936; Mab 22C10; mAb-22C10; mAb22c10; Map-1B; MAP-IB; Map1B; Olk; ssC10
Alternate Antibody Name:
Alternate Antigen Name: MAP1B homolog
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: aa 2517 to 2758 of futsch
Uniprot ID: B4R2P4
Entrez Gene ID: 6724864
Antibody Registry ID: AB_528403
Additional Information: RRID: AB_528403
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22C10 was deposited to the DSHB by Benzer, S. / Colley, N. (DSHB Hybridoma Product 22C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References