DN-Ex #8
Catalog Fields
Product Name/ID: DN-Ex #8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CadN
Ab Isotype: RIgG2a
Gene Symbol:
Entrez Gene ID: 35070
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3-X63-Ag8-U1
Epitope Mapped: Yes
Antigen Name: Cadherin, DN- (extracellular domain)
Epitope Location or Sequence: extracellular region including CR2-CR8
Alternate Antigen Name:
Deposit Date: 1/7/2004
Antigen Molecular Weight: Predicted: 347 kDa; Apparent: 300 and 200 kDa
Depositor: Uemura, T.
Antigen Sequence:
Depositor Institution: Kyoto Univ. Graduate Sch.of Biostudies
Antigen Species: Drosophila
Depositor Notes: Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Marker of neuropil
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DN-Ex #8 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product DN-Ex #8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
25 References
Initial Publication
Axon patterning requires DN-cadherin, a novel neuronal adhesion receptor, in the Drosophila embryonic CNS.
Uemura T
Neuron 19.1 (1997 Jul): 77-89.