Clone ID / Name: DN-Ex #8

Anti - Cadherin, DN- (extracellular domain)

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$50.00

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SKU: DN-Ex #8

Product Form :

Supernatant 1.0 ml

~ 19 – 29 μg/mL

Concentrate 0.1 ml

~ 222 – 247 μg/mL

Frozen Cells 1 vial

N/A

Growing Cells 1 vial

N/A

iConcentration varies between lots. Lots are distributed in the order they are produced.

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Catalog Fields

Identity

Clone ID / Name: DN-Ex #8

Alternate Antibody Name: —

Antigen Name: Cadherin, DN- (extracellular domain)

Alternate Antigen Name: —

Gene Symbol: —

Gene Name: CadN

Entrez Gene ID: 35070

Antigen Sequence: —

Antigen Species: Drosophila

Antigen Mol. Weight: Predicted: 347 kDa; Apparent: 300 and 200 kDa

Uniprot ID: O15943

Human Protein Atlas: —

Antibody Properties

Host Species: Rat

Clonality: Monoclonal

Isotype: RIgG2a

Myeloma Strain: P3-X63-Ag8-U1

Antibody Registry / RRID: AB_528121

Available to For-Profits: Yes

Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.

Immunogen & Epitope

Immunogen: Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8.

Immunogen Sequence: Partial protein

Epitope Mapped: Yes

Epitope Location / Seq: extracellular region including CR2-CR8

Clone: —

Additional Info: Marker of neuropil

Add. Characterization: —

Species Reactivity

Confirmed Reactivity: Drosophila

Predicted Reactivity: —

Depositor

Depositor Name: T. Uemura

Institution: Kyoto Univ. Graduate Sch.of Biostudies

Deposit Date: January 7, 2004

Depositor Notes: Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions.

Recommended Applications

IHC

Each lab must optimize concentration per application and species. Full usage recommendations →

UniProt DataUniProtO15943 ↗

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Could not load UniProt data for O15943

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Clone ID / Name: DN-Ex #8

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