DN-Ex #8

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$40.00
SKU: DN-Ex #8
View product citations for antibody DN-Ex #8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin, DN- (extracellular domain)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Uemura, T.
Isotype: RIgG2a
Depositors Institution: Kyoto Univ. Graduate Sch.of Biostudies
Host Species: rat
Depositors Notes: Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions.
Positive Tested Species Reactivity: Drosophila
Gene: CadN
Antigen Molecular Weight: Predicted: 347 kDa; Apparent: 300 and 200 kDa
Alternate Gene Names: CG7100, N-cadh
Immunogen: Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8.
Clonality: Monoclonal
Myeloma Strain: P3-X63-Ag8-U1
Epitope Mapped: Yes
Uniprot ID: O15943 
Epitope Location or Sequence: extracellular region including CR2-CR8
Entrez Gene ID: 35070 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528121 
Additional Information: Marker of neuropil
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DN-Ex #8 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product DN-Ex #8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Axon patterning requires DN-cadherin, a novel neuronal adhesion receptor, in the Drosophila embryonic CNS.
    Uemura T
    Neuron 19.1 (1997 Jul): 77-89.

    The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis.
    Luo L
    Cell 141.3 (2010 Apr 30): 536-48.

    Arborization pattern of engrailed-positive neural lineages reveal neuromere boundaries in the Drosophila brain neuropil.
    Hartenstein V
    The Journal of comparative neurology 517.1 (2009 Nov 1): 87-104.

    Postembryonic lineages of the Drosophila brain: I. Development of the lineage-associated fiber tracts.
    Hartenstein V
    Developmental biology 384.2 (2013 Dec 15): 228-57.

    Origin and development of neuropil glia of the Drosophila larval and adult brain: Two distinct glial populations derived from separate progenitors.
    Hartenstein V
    Developmental biology 404.2 (2015 Aug 15): 2-20.

    Mechanism of Notch Pathway Activation and Its Role in the Regulation of Olfactory Plasticity in Drosophila melanogaster.
    Lieber T
    PloS one 11.3 (2016): e0151279.

    doublesex functions early and late in gustatory sense organ development.
    Baker BS
    PloS one 7.12 (2012): e51489.

    Lineage-based analysis of the development of the central complex of the Drosophila brain.
    Hartenstein V
    The Journal of comparative neurology 519.4 (2011 Mar 1): 661-89.

    MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.
    Bellen HJ
    Nature methods 8.9 (2011 Sep): 737-43.

    Building the central complex in Drosophila: the generation and development of distinct neural subsets.
    Armstrong JD
    The Journal of comparative neurology 518.9 (2010 May 1): 1525-41.

    Modular neuropile organization in the Drosophila larval brain facilitates identification and mapping of central neurons.
    Atwood HL
    The Journal of comparative neurology 499.4 (2006 Dec 1): 583-602.

    Haemocyte-derived SPARC is required for collagen-IV-dependent stability of basal laminae in Drosophila embryos.
    Ringuette M
    Journal of cell science 121.Pt 10 (2008 May 15): 1671-80.

    Bazooka mediates secondary axon morphology in Drosophila brain lineages.
    Hartenstein V
    Neural development 6. (2011 Apr 27): 16.

    The Arf-GEF Schizo/Loner regulates N-cadherin to induce fusion competence of Drosophila myoblasts.
    Önel SF
    Developmental biology 368.1 (2012 Aug 1): 18-27.

    Dissection of the <i>Drosophila</i> neuropeptide F circuit using a high-throughput two-choice assay.
    Heberlein U
    Proceedings of the National Academy of Sciences of the United States of America 114.38 (2017 Sep 19): E8091-E8099.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres.
    Truman JW
    The Journal of comparative neurology 524.13 (2016 Sep 1): 2677-95.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Revisiting the developmental and cellular role of the pigmentation gene yellow in Drosophila using a tagged allele.
    Gompel N
    Developmental biology 438.2 (2018 Jun 15): 111-123.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

    WB References
    IHC References
    Epitope Map References
    All References

    Axon patterning requires DN-cadherin, a novel neuronal adhesion receptor, in the Drosophila embryonic CNS.
    Uemura T
    Neuron 19.1 (1997 Jul): 77-89.

    DN-cadherin is required for spatial arrangement of nerve terminals and ultrastructural organization of synapses.
    Uemura T
    Molecular and cellular neurosciences 19.3 (2002 Mar): 375-88.

    MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.
    Bellen HJ
    Nature methods 8.9 (2011 Sep): 737-43.

    Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements.
    Treisman JE
    Developmental cell 50.3 (2019 Aug 5): 313-326.e5.

    The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis.
    Luo L
    Cell 141.3 (2010 Apr 30): 536-48.

    Arborization pattern of engrailed-positive neural lineages reveal neuromere boundaries in the Drosophila brain neuropil.
    Hartenstein V
    The Journal of comparative neurology 517.1 (2009 Nov 1): 87-104.

    Postembryonic lineages of the Drosophila brain: I. Development of the lineage-associated fiber tracts.
    Hartenstein V
    Developmental biology 384.2 (2013 Dec 15): 228-57.

    Origin and development of neuropil glia of the Drosophila larval and adult brain: Two distinct glial populations derived from separate progenitors.
    Hartenstein V
    Developmental biology 404.2 (2015 Aug 15): 2-20.

    Mechanism of Notch Pathway Activation and Its Role in the Regulation of Olfactory Plasticity in Drosophila melanogaster.
    Lieber T
    PloS one 11.3 (2016): e0151279.

    doublesex functions early and late in gustatory sense organ development.
    Baker BS
    PloS one 7.12 (2012): e51489.

    Lineage-based analysis of the development of the central complex of the Drosophila brain.
    Hartenstein V
    The Journal of comparative neurology 519.4 (2011 Mar 1): 661-89.

    Building the central complex in Drosophila: the generation and development of distinct neural subsets.
    Armstrong JD
    The Journal of comparative neurology 518.9 (2010 May 1): 1525-41.

    Modular neuropile organization in the Drosophila larval brain facilitates identification and mapping of central neurons.
    Atwood HL
    The Journal of comparative neurology 499.4 (2006 Dec 1): 583-602.

    Haemocyte-derived SPARC is required for collagen-IV-dependent stability of basal laminae in Drosophila embryos.
    Ringuette M
    Journal of cell science 121.Pt 10 (2008 May 15): 1671-80.

    Bazooka mediates secondary axon morphology in Drosophila brain lineages.
    Hartenstein V
    Neural development 6. (2011 Apr 27): 16.

    The Arf-GEF Schizo/Loner regulates N-cadherin to induce fusion competence of Drosophila myoblasts.
    Önel SF
    Developmental biology 368.1 (2012 Aug 1): 18-27.

    Dissection of the <i>Drosophila</i> neuropeptide F circuit using a high-throughput two-choice assay.
    Heberlein U
    Proceedings of the National Academy of Sciences of the United States of America 114.38 (2017 Sep 19): E8091-E8099.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres.
    Truman JW
    The Journal of comparative neurology 524.13 (2016 Sep 1): 2677-95.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Revisiting the developmental and cellular role of the pigmentation gene yellow in Drosophila using a tagged allele.
    Gompel N
    Developmental biology 438.2 (2018 Jun 15): 111-123.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

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