DN-Ex #8
Catalog Fields
Clone ID/Product Name: DN-Ex #8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: CadN
Ab Isotype: RIgG2a
Gene Name:
Entrez Gene ID: 35070
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3-X63-Ag8-U1
Epitope Mapped: Yes
Antigen Name: Cadherin, DN- (extracellular domain)
Epitope Location or Sequence: extracellular region including CR2-CR8
Alternate Antigen Name:
Deposit Date: 1/7/2004
Antigen Molecular Weight: Predicted: 347 kDa; Apparent: 300 and 200 kDa
Depositor: Uemura, T.
Antigen Sequence:
Depositor Institution: Kyoto Univ. Graduate Sch.of Biostudies
Antigen Species: Drosophila
Depositor Notes: Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions.
Host Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Marker of neuropil
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot