DN-Ex #8

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SKU: DN-Ex #8
View product citations for antibody DN-Ex #8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin, DN- (extracellular domain)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Uemura, T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Kyoto Univ. Graduate Sch.of Biostudies
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions.
Antigen Molecular Weight: Predicted: 347 kDa; Apparent: 300 and 200 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CadN
Immunogen: Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8.
Alternate Gene Names: CG7100, N-cadh
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8-U1
Epitope Location or Sequence: extracellular region including CR2-CR8
Uniprot ID: O15943 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35070 
Additional Characterization:
Antibody Registry ID: AB_528121 
Additional Information: Marker of neuropil
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DN-Ex #8 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product DN-Ex #8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References
    All References

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