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SKU: 81.5C10
View product citations for antibody 81.5C10 on CiteAb

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Available: 532

DSHB Data Sheet

Catalog Fields

Antigen: MNR2/HB9/Mnx1
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Fish, Human, Mouse, Zebrafish
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MNR2
Immunogen: MNR2 NH2(C-terminus; chick)-GST fusion protein expressed in E. coli.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q9YHY8 
Immunogen Sequence:
Entrez Gene ID: 395769 
Additional Characterization:
Antibody Registry ID: AB_2145209 
Additional Information: recognize Mnx1 (mouse, PMID: 25179941)
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
81.5C10 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 81.5C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

48 References

  • Initial Publication
  • IF References
  • IHC References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References
    IHC References
    Gel Supershift References
    All References

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