F25A1B3

(0) No Reviews yet
$40.00
SKU: F25A1B3
View product citations for antibody F25A1B3 on CiteAb

In Stock

Available: 158

DSHB Data Sheet

Catalog Fields

Antigen: neurogenin 3
Hybridoma Cells Available:
Antigen Species: Mouse
Depositor: Madsen, O.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hagedorn Research Institute, Denmark
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Fusion: 11/6/2003. Basic helix-loop-helix transcription factor required for pancreatic endocrine development. Expressed transiently during mouse pancreas development.
Antigen Molecular Weight: Predicted: 23kDa; Apparent: 30-35kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Neurog3
Immunogen: Recombinant partial Ngn3; GST-Ngn3 fusion protein
Alternate Gene Names: ngn3; Atoh5; Math4B; bHLHa7; Ath4b
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: aa 1-95
Uniprot ID: P70661 
Immunogen Sequence: aa 1-95
Entrez Gene ID: 11925 
Additional Characterization:
Antibody Registry ID: AB_528401 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F25A1B3 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F25A1B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

29 References

  • Initial Publication
  • IF References
  • WB References
  • FFPE References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Generation of monoclonal antibodies against mouse neurogenin 3: a new immunocytochemical tool to study the pancreatic endocrine progenitor cell.
    Madsen O
    Hybridoma and hybridomics 23.6 (2004 Dec): 385-8.

    Neurogenin 3 cellular and subcellular localization in the developing and adult hippocampus.
    Arevalo MA
    The Journal of comparative neurology 518.10 (2010 May 15): 1814-24.

    Global gene expression profiling and histochemical analysis of the developing human fetal pancreas.
    Hutton JC
    Diabetologia 51.2 (2008 Feb): 285-97.

    Mouse atonal homolog 1 directs intestinal progenitors to secretory cell rather than absorptive cell fate.
    Samuelson LC
    Developmental biology 346.2 (2010 Oct 15): 215-23.

    The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors.
    Rane SG
    Development (Cambridge, England) 138.10 (2011 May): 1903-12.

    Tumour suppressor menin is essential for development of the pancreatic endocrine cells.
    Zhang CX
    The Journal of endocrinology 199.2 (2008 Nov): 287-98.

    Notch/neurogenin 3 signalling is involved in the neuritogenic actions of oestradiol in developing hippocampal neurones.
    Arevalo MA
    Journal of neuroendocrinology 23.4 (2011 Apr): 355-64.

    Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation.
    Lan MS
    Journal of cellular physiology 227.6 (2012 Jun): 2470-9.

    Embryonic stem cell transplantation correlates with endogenous neurogenin 3 expression and pancreas regeneration in streptozotocin-injured mice.
    Quinn G
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.12 (2009 Dec): 1149-58.

    Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
    Jay P
    The Journal of cell biology 192.5 (2011 Mar 7): 767-80.

    Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas.
    Kume S
    Genes to cells : devoted to molecular and cellular mechanisms 13.7 (2008 Jul): 667-78.

    Loss of Myt1 function partially compromises endocrine islet cell differentiation and pancreatic physiological function in the mouse.
    Gu G
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 898-910.

    A CRM1-mediated nuclear export signal is essential for cytoplasmic localization of neurogenin 3 in neurons.
    Arevalo MA
    PloS one 8.1 (2013): e55237.

    Dynamic regulation of Pdx1 enhancers by Foxa1 and Foxa2 is essential for pancreas development.
    Kaestner KH
    Genes and development 22.24 (2008 Dec 15): 3435-48.

    Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas.
    Kaestner KH
    Mechanisms of development 126.8-9 (2009 Aug-Sep): 687-99.

    Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system.
    Lewis J
    PloS one 6.9 (2011): e24484.

    GATA4 and GATA6 control mouse pancreas organogenesis.
    Rojas A
    The Journal of clinical investigation 122.10 (2012 Oct): 3504-15.

    ADAM10 regulates Notch function in intestinal stem cells of mice.
    Dempsey PJ
    Gastroenterology 147.4 (2014 Oct): 822-834.e13.

    Generation of functional insulin-producing cells in the gut by Foxo1 ablation.
    Accili D
    Nature genetics 44.4 (2012 Mar 11): 406-12, S1.

    Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Kim SK
    eLife 2. (2013 Nov 19): e00940.

    Novel effectors of directed and Ngn3-mediated differentiation of mouse embryonic stem cells into endocrine pancreas progenitors.
    Gavalas A
    Stem cells (Dayton, Ohio) 26.1 (2008 Jan): 3-16.

    Neurogenin3 cooperates with Foxa2 to autoactivate its own expression.
    Gasa R
    The Journal of biological chemistry 288.17 (2013 Apr 26): 11705-17.

    Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas.
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1.
    Notkins AL
    Endocrinology 152.5 (2011 May): 1961-9.

    Glial cell line-derived neurotrophic factor enhances neurogenin3 gene expression and beta-cell proliferation in the developing mouse pancreas.
    Srinivasan S
    American journal of physiology. Gastrointestinal and liver physiology 299.1 (2010 Jul): G283-92.

    SEC23B is required for the maintenance of murine professional secretory tissues.
    Zhang B
    Proceedings of the National Academy of Sciences of the United States of America 109.29 (2012 Jul 17): E2001-9.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    WB References
    FFPE References
    Epitope Map References
    Gel Supershift References
    All References

    Generation of monoclonal antibodies against mouse neurogenin 3: a new immunocytochemical tool to study the pancreatic endocrine progenitor cell.
    Madsen O
    Hybridoma and hybridomics 23.6 (2004 Dec): 385-8.

    Neurogenin 3 cellular and subcellular localization in the developing and adult hippocampus.
    Arevalo MA
    The Journal of comparative neurology 518.10 (2010 May 15): 1814-24.

    Global gene expression profiling and histochemical analysis of the developing human fetal pancreas.
    Hutton JC
    Diabetologia 51.2 (2008 Feb): 285-97.

    Mouse atonal homolog 1 directs intestinal progenitors to secretory cell rather than absorptive cell fate.
    Samuelson LC
    Developmental biology 346.2 (2010 Oct 15): 215-23.

    The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors.
    Rane SG
    Development (Cambridge, England) 138.10 (2011 May): 1903-12.

    Tumour suppressor menin is essential for development of the pancreatic endocrine cells.
    Zhang CX
    The Journal of endocrinology 199.2 (2008 Nov): 287-98.

    Notch/neurogenin 3 signalling is involved in the neuritogenic actions of oestradiol in developing hippocampal neurones.
    Arevalo MA
    Journal of neuroendocrinology 23.4 (2011 Apr): 355-64.

    Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation.
    Lan MS
    Journal of cellular physiology 227.6 (2012 Jun): 2470-9.

    Embryonic stem cell transplantation correlates with endogenous neurogenin 3 expression and pancreas regeneration in streptozotocin-injured mice.
    Quinn G
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.12 (2009 Dec): 1149-58.

    Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
    Jay P
    The Journal of cell biology 192.5 (2011 Mar 7): 767-80.

    Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas.
    Kume S
    Genes to cells : devoted to molecular and cellular mechanisms 13.7 (2008 Jul): 667-78.

    Loss of Myt1 function partially compromises endocrine islet cell differentiation and pancreatic physiological function in the mouse.
    Gu G
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 898-910.

    A CRM1-mediated nuclear export signal is essential for cytoplasmic localization of neurogenin 3 in neurons.
    Arevalo MA
    PloS one 8.1 (2013): e55237.

    Dynamic regulation of Pdx1 enhancers by Foxa1 and Foxa2 is essential for pancreas development.
    Kaestner KH
    Genes and development 22.24 (2008 Dec 15): 3435-48.

    Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas.
    Kaestner KH
    Mechanisms of development 126.8-9 (2009 Aug-Sep): 687-99.

    Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system.
    Lewis J
    PloS one 6.9 (2011): e24484.

    GATA4 and GATA6 control mouse pancreas organogenesis.
    Rojas A
    The Journal of clinical investigation 122.10 (2012 Oct): 3504-15.

    ADAM10 regulates Notch function in intestinal stem cells of mice.
    Dempsey PJ
    Gastroenterology 147.4 (2014 Oct): 822-834.e13.

    Generation of functional insulin-producing cells in the gut by Foxo1 ablation.
    Accili D
    Nature genetics 44.4 (2012 Mar 11): 406-12, S1.

    Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Kim SK
    eLife 2. (2013 Nov 19): e00940.

    Novel effectors of directed and Ngn3-mediated differentiation of mouse embryonic stem cells into endocrine pancreas progenitors.
    Gavalas A
    Stem cells (Dayton, Ohio) 26.1 (2008 Jan): 3-16.

    Neurogenin3 cooperates with Foxa2 to autoactivate its own expression.
    Gasa R
    The Journal of biological chemistry 288.17 (2013 Apr 26): 11705-17.

    Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas.
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1.
    Notkins AL
    Endocrinology 152.5 (2011 May): 1961-9.

    Glial cell line-derived neurotrophic factor enhances neurogenin3 gene expression and beta-cell proliferation in the developing mouse pancreas.
    Srinivasan S
    American journal of physiology. Gastrointestinal and liver physiology 299.1 (2010 Jul): G283-92.

    SEC23B is required for the maintenance of murine professional secretory tissues.
    Zhang B
    Proceedings of the National Academy of Sciences of the United States of America 109.29 (2012 Jul 17): E2001-9.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    Polyswitch lentivectors: "all-in-one" lentiviral vectors for drug-inducible gene expression, live selection, and recombination cloning.
    Salmon P
    Human gene therapy 22.10 (2011 Oct): 1255-67.

    Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1.
    Lan MS
    FEBS letters 581.5 (2007 Mar 6): 949-54.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review