F25A1B3

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SKU: F25A1B3-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: F25A1B3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Neurog3
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_528401 
Uniprot ID: P70661 
RRID:  
Entrez Gene ID: 11925 
Clonality: Monoclonal
Immunogen: Recombinant partial Ngn3; GST-Ngn3 fusion protein
Clone:
Immunogen Sequence: aa 1-95
Myeloma Strain: Fox-NY
Epitope Mapped: Yes
Antigen Name: neurogenin 3
Epitope Location or Sequence: aa 1-95
Alternate Antigen Name:
Deposit Date: 6/22/2005
Antigen Molecular Weight: Predicted: 23kDa; Apparent: 30-35kDa
Depositor: Madsen, O.D.
Antigen Sequence:
Depositor Institution: Hagedorn Research Institute
Antigen Species: Mouse
Depositor Notes: Fusion: 11/6/2003. Basic helix-loop-helix transcription factor required for pancreatic endocrine development. Expressed transiently during mouse pancreas development.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F25A1B3 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F25A1B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

31 References

  • Initial Publication
  • IF References
  • WB References
  • ChIP References
  • FFPE References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Generation of monoclonal antibodies against mouse neurogenin 3: a new immunocytochemical tool to study the pancreatic endocrine progenitor cell.
    Madsen O
    Hybridoma and hybridomics 23.6 (2004 Dec): 385-8.

    Neurogenin 3 cellular and subcellular localization in the developing and adult hippocampus.
    Arevalo MA
    The Journal of comparative neurology 518.10 (2010 May 15): 1814-24.

    Global gene expression profiling and histochemical analysis of the developing human fetal pancreas.
    Hutton JC
    Diabetologia 51.2 (2008 Feb): 285-97.

    Mouse atonal homolog 1 directs intestinal progenitors to secretory cell rather than absorptive cell fate.
    Samuelson LC
    Developmental biology 346.2 (2010 Oct 15): 215-23.

    The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors.
    Rane SG
    Development (Cambridge, England) 138.10 (2011 May): 1903-12.

    Tumour suppressor menin is essential for development of the pancreatic endocrine cells.
    Zhang CX
    The Journal of endocrinology 199.2 (2008 Nov): 287-98.

    Notch/neurogenin 3 signalling is involved in the neuritogenic actions of oestradiol in developing hippocampal neurones.
    Arevalo MA
    Journal of neuroendocrinology 23.4 (2011 Apr): 355-64.

    Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation.
    Lan MS
    Journal of cellular physiology 227.6 (2012 Jun): 2470-9.

    Embryonic stem cell transplantation correlates with endogenous neurogenin 3 expression and pancreas regeneration in streptozotocin-injured mice.
    Quinn G
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.12 (2009 Dec): 1149-58.

    Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
    Jay P
    The Journal of cell biology 192.5 (2011 Mar 7): 767-80.

    Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas.
    Kume S
    Genes to cells : devoted to molecular & cellular mechanisms 13.7 (2008 Jul): 667-78.

    Loss of Myt1 function partially compromises endocrine islet cell differentiation and pancreatic physiological function in the mouse.
    Gu G
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 898-910.

    A CRM1-mediated nuclear export signal is essential for cytoplasmic localization of neurogenin 3 in neurons.
    Arevalo MA
    PloS one 8.1 (2013): e55237.

    Dynamic regulation of Pdx1 enhancers by Foxa1 and Foxa2 is essential for pancreas development.
    Kaestner KH
    Genes & development 22.24 (2008 Dec 15): 3435-48.

    Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas.
    Kaestner KH
    Mechanisms of development 126.8-9 (2009 Aug-Sep): 687-99.

    Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system.
    Lewis J
    PloS one 6.9 (2011): e24484.

    GATA4 and GATA6 control mouse pancreas organogenesis.
    Rojas A
    The Journal of clinical investigation 122.10 (2012 Oct): 3504-15.

    ADAM10 regulates Notch function in intestinal stem cells of mice.
    Dempsey PJ
    Gastroenterology 147.4 (2014 Oct): 822-834.e13.

    Generation of functional insulin-producing cells in the gut by Foxo1 ablation.
    Accili D
    Nature genetics 44.4 (2012 Mar 11): 406-12, S1.

    Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Kim SK
    eLife 2. (2013 Nov 19): e00940.

    Novel effectors of directed and Ngn3-mediated differentiation of mouse embryonic stem cells into endocrine pancreas progenitors.
    Gavalas A
    Stem cells (Dayton, Ohio) 26.1 (2008 Jan): 3-16.

    Neurogenin3 cooperates with Foxa2 to autoactivate its own expression.
    Gasa R
    The Journal of biological chemistry 288.17 (2013 Apr 26): 11705-17.

    Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas.
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1.
    Notkins AL
    Endocrinology 152.5 (2011 May): 1961-9.

    Glial cell line-derived neurotrophic factor enhances neurogenin3 gene expression and beta-cell proliferation in the developing mouse pancreas.
    Srinivasan S
    American journal of physiology. Gastrointestinal and liver physiology 299.1 (2010 Jul): G283-92.

    SEC23B is required for the maintenance of murine professional secretory tissues.
    Zhang B
    Proceedings of the National Academy of Sciences of the United States of America 109.29 (2012 Jul 17): E2001-9.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    PDX1(LOW) MAFA(LOW) β-cells contribute to islet function and insulin release.
    Hodson DJ
    Nature communications 12.1 (2021 Jan 29): 674.

    The SNAG Domain of Insm1 Regulates Pancreatic Endocrine Cell Differentiation and Represses β- to δ-Cell Transdifferentiation.
    Jia S
    Diabetes 70.5 (2021 May): 1084-1097.

    WB References
    ChIP References
    FFPE References
    Epitope Map References
    Gel Supershift References
    All References

    Generation of monoclonal antibodies against mouse neurogenin 3: a new immunocytochemical tool to study the pancreatic endocrine progenitor cell.
    Madsen O
    Hybridoma and hybridomics 23.6 (2004 Dec): 385-8.

    Neurogenin 3 cellular and subcellular localization in the developing and adult hippocampus.
    Arevalo MA
    The Journal of comparative neurology 518.10 (2010 May 15): 1814-24.

    Global gene expression profiling and histochemical analysis of the developing human fetal pancreas.
    Hutton JC
    Diabetologia 51.2 (2008 Feb): 285-97.

    Mouse atonal homolog 1 directs intestinal progenitors to secretory cell rather than absorptive cell fate.
    Samuelson LC
    Developmental biology 346.2 (2010 Oct 15): 215-23.

    The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors.
    Rane SG
    Development (Cambridge, England) 138.10 (2011 May): 1903-12.

    Tumour suppressor menin is essential for development of the pancreatic endocrine cells.
    Zhang CX
    The Journal of endocrinology 199.2 (2008 Nov): 287-98.

    Notch/neurogenin 3 signalling is involved in the neuritogenic actions of oestradiol in developing hippocampal neurones.
    Arevalo MA
    Journal of neuroendocrinology 23.4 (2011 Apr): 355-64.

    Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation.
    Lan MS
    Journal of cellular physiology 227.6 (2012 Jun): 2470-9.

    Embryonic stem cell transplantation correlates with endogenous neurogenin 3 expression and pancreas regeneration in streptozotocin-injured mice.
    Quinn G
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.12 (2009 Dec): 1149-58.

    Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
    Jay P
    The Journal of cell biology 192.5 (2011 Mar 7): 767-80.

    Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas.
    Kume S
    Genes to cells : devoted to molecular & cellular mechanisms 13.7 (2008 Jul): 667-78.

    Loss of Myt1 function partially compromises endocrine islet cell differentiation and pancreatic physiological function in the mouse.
    Gu G
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 898-910.

    A CRM1-mediated nuclear export signal is essential for cytoplasmic localization of neurogenin 3 in neurons.
    Arevalo MA
    PloS one 8.1 (2013): e55237.

    Dynamic regulation of Pdx1 enhancers by Foxa1 and Foxa2 is essential for pancreas development.
    Kaestner KH
    Genes & development 22.24 (2008 Dec 15): 3435-48.

    Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas.
    Kaestner KH
    Mechanisms of development 126.8-9 (2009 Aug-Sep): 687-99.

    Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system.
    Lewis J
    PloS one 6.9 (2011): e24484.

    GATA4 and GATA6 control mouse pancreas organogenesis.
    Rojas A
    The Journal of clinical investigation 122.10 (2012 Oct): 3504-15.

    ADAM10 regulates Notch function in intestinal stem cells of mice.
    Dempsey PJ
    Gastroenterology 147.4 (2014 Oct): 822-834.e13.

    Generation of functional insulin-producing cells in the gut by Foxo1 ablation.
    Accili D
    Nature genetics 44.4 (2012 Mar 11): 406-12, S1.

    Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Kim SK
    eLife 2. (2013 Nov 19): e00940.

    Novel effectors of directed and Ngn3-mediated differentiation of mouse embryonic stem cells into endocrine pancreas progenitors.
    Gavalas A
    Stem cells (Dayton, Ohio) 26.1 (2008 Jan): 3-16.

    Neurogenin3 cooperates with Foxa2 to autoactivate its own expression.
    Gasa R
    The Journal of biological chemistry 288.17 (2013 Apr 26): 11705-17.

    Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas.
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1.
    Notkins AL
    Endocrinology 152.5 (2011 May): 1961-9.

    Glial cell line-derived neurotrophic factor enhances neurogenin3 gene expression and beta-cell proliferation in the developing mouse pancreas.
    Srinivasan S
    American journal of physiology. Gastrointestinal and liver physiology 299.1 (2010 Jul): G283-92.

    SEC23B is required for the maintenance of murine professional secretory tissues.
    Zhang B
    Proceedings of the National Academy of Sciences of the United States of America 109.29 (2012 Jul 17): E2001-9.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    PDX1(LOW) MAFA(LOW) β-cells contribute to islet function and insulin release.
    Hodson DJ
    Nature communications 12.1 (2021 Jan 29): 674.

    The SNAG Domain of Insm1 Regulates Pancreatic Endocrine Cell Differentiation and Represses β- to δ-Cell Transdifferentiation.
    Jia S
    Diabetes 70.5 (2021 May): 1084-1097.

    Polyswitch lentivectors: "all-in-one" lentiviral vectors for drug-inducible gene expression, live selection, and recombination cloning.
    Salmon P
    Human gene therapy 22.10 (2011 Oct): 1255-67.

    Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1.
    Lan MS
    FEBS letters 581.5 (2007 Mar 6): 949-54.

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