D7F2

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SKU: D7F2

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: D7F2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: myod1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_1157912 
Uniprot ID: Q7T109 
RRID:  
Entrez Gene ID: 394569 
Clonality: Monoclonal
Immunogen: amino acids 3-195 of XMyoD, fused to GST
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: MyoD
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/16/2007
Antigen Molecular Weight:
Depositor: Gurdon, J. / Standley, H.J.
Antigen Sequence:
Depositor Institution: University of Cambridge
Antigen Species: Xenopus laevis
Depositor Notes: Recognizes XMyoDa and XMyoDb
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Frog, Xenopus
Additional Information: RRID:AB_1157912
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D7F2 was deposited to the DSHB by Gurdon, J. / Standley, H.J. (DSHB Hybridoma Product D7F2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • WB References
  • IHC References
  • ChIP References
  • All References
  • Initial Publication

    Expression of XMyoD protein in early Xenopus laevis embryos.
    Dilworth SM
    Development (Cambridge, England) 114.1 (1992 Jan): 31-8.

    WB References

    Expression of XMyoD protein in early Xenopus laevis embryos.
    Dilworth SM
    Development (Cambridge, England) 114.1 (1992 Jan): 31-8.

    Early transcriptional targets of MyoD link myogenesis and somitogenesis.
    Pownall ME
    Developmental biology 371.2 (2012 Nov 15): 256-68.

    IHC References

    eFGF and its mode of action in the community effect during Xenopus myogenesis.
    Gurdon JB
    Development (Cambridge, England) 128.8 (2001 Apr): 1347-57.

    ChIP References

    Early transcriptional targets of MyoD link myogenesis and somitogenesis.
    Pownall ME
    Developmental biology 371.2 (2012 Nov 15): 256-68.

    All References

    eFGF and its mode of action in the community effect during Xenopus myogenesis.
    Gurdon JB
    Development (Cambridge, England) 128.8 (2001 Apr): 1347-57.

    Expression of XMyoD protein in early Xenopus laevis embryos.
    Dilworth SM
    Development (Cambridge, England) 114.1 (1992 Jan): 31-8.

    Early transcriptional targets of MyoD link myogenesis and somitogenesis.
    Pownall ME
    Developmental biology 371.2 (2012 Nov 15): 256-68.

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