D7F2

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$40.00
SKU: D7F2
View product citations for antibody D7F2 on CiteAb

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Available: 138

DSHB Data Sheet

Catalog Fields

Antigen: MyoD
Hybridoma Cells Available: Yes
Antigen Species: Xenopus laevis
Depositor: Gurdon, J. / Standley, H.J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Frog, Xenopus
Depositors Notes: Recognizes XMyoDa and XMyoDb
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: myod1
Immunogen: amino acids 3-195 of XMyoD, fused to GST
Alternate Gene Names: myogenic factor 3, MyoD, XtmyoD, XMyoD, mf25
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q7T109 
Immunogen Sequence:
Entrez Gene ID: 394569 
Additional Characterization:  
Antibody Registry ID: AB_1157912 
Additional Information: RRID:AB_1157912
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D7F2 was deposited to the DSHB by Gurdon, J. / Standley, H.J. (DSHB Hybridoma Product D7F2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Expression of XMyoD protein in early Xenopus laevis embryos.
    Dilworth SM
    Development (Cambridge, England) 114.1 (1992 Jan): 31-8.

    WB References

    Expression of XMyoD protein in early Xenopus laevis embryos.
    Dilworth SM
    Development (Cambridge, England) 114.1 (1992 Jan): 31-8.

    Early transcriptional targets of MyoD link myogenesis and somitogenesis.
    Pownall ME
    Developmental biology 371.2 (2012 Nov 15): 256-68.

    IHC References

    eFGF and its mode of action in the community effect during Xenopus myogenesis.
    Gurdon JB
    Development (Cambridge, England) 128.8 (2001 Apr): 1347-57.

    All References

    eFGF and its mode of action in the community effect during Xenopus myogenesis.
    Gurdon JB
    Development (Cambridge, England) 128.8 (2001 Apr): 1347-57.

    Expression of XMyoD protein in early Xenopus laevis embryos.
    Dilworth SM
    Development (Cambridge, England) 114.1 (1992 Jan): 31-8.

    Early transcriptional targets of MyoD link myogenesis and somitogenesis.
    Pownall ME
    Developmental biology 371.2 (2012 Nov 15): 256-68.

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