9 D10

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$40.00
SKU: 9 D10
View product citations for antibody 9 D10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Titin
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Greaser, M.L.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wisconsin-Madison
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Drosophila, Fish, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Xenopus
Depositors Notes: Stains 4% paraformaldehyde or methanol fixed cells and paraffin sections; also 0.1% glutaraldehyde
Antigen Molecular Weight: 3000-4000 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: TTN
Immunogen: Titin (purified) bovine heart.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: proline-glutamate-valine-lysine (PEVK) domain;
Uniprot ID: F1N757 
Immunogen Sequence:
Entrez Gene ID: 540561 
Additional Characterization:
Antibody Registry ID: AB_528491 
Additional Information: single titin molecule labeling PMID: 24357719; immunoelectron microscopy PMID: 9724620 9277364 9172076
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9 D10 was deposited to the DSHB by Greaser, M.L. (DSHB Hybridoma Product 9 D10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

36 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils.
    Greaser ML
    Journal of muscle research and cell motility 6.3 (1985 Jun): 293-312.

    IF References

    Left ventricular torsional mechanics and myocardial iron load in beta-thalassaemia major: a potential role of titin degradation.
    Cheung YF
    BMC cardiovascular disorders 14. (2014 Apr 12): 49.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    Heterogeneity of myotubes generated by the MyoD and E12 basic helix-loop-helix transcription factors in otherwise non-differentiation growth conditions.
    Parpura V
    Biomaterials 35.7 (2014 Feb): 2188-98.

    Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging.
    Gregorio CC
    Methods (San Diego, Calif.) 66.3 (2014 Apr 1): 370-9.

    Critical roles for multiple formins during cardiac myofibril development and repair.
    Goode BL
    Molecular biology of the cell 25.6 (2014 Mar): 811-27.

    DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila.
    Mihály J
    PLoS genetics 10.2 (2014 Feb): e1004166.

    Epicardium is required for sarcomeric maturation and cardiomyocyte growth in the ventricular compact layer mediated by transforming growth factor β and fibroblast growth factor before the onset of coronary circulation.
    Nakajima Y
    Congenital anomalies 54.3 (2014 Aug): 162-71.

    Titin is a target of matrix metalloproteinase-2: implications in myocardial ischemia/reperfusion injury.
    Schulz R
    Circulation 122.20 (2010 Nov 16): 2039-47.

    Pathways of Ca²⁺ entry and cytoskeletal damage following eccentric contractions in mouse skeletal muscle.
    Allen DG
    Journal of applied physiology (Bethesda, Md. : 1985) 112.12 (2012 Jun): 2077-86.

    Comparison of cardiomyogenic potential among human ESC and iPSC lines.
    Lough JW
    Cell transplantation 21.11 (2012): 2523-30.

    Transcription factor rational design improves directed differentiation of human mesenchymal stem cells into skeletal myocytes.
    de Vries AA
    Molecular therapy : the journal of the American Society of Gene Therapy 19.7 (2011 Jul): 1331-41.

    Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload.
    Ross RS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4252-64.

    A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation.
    Martin U
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 463-73.

    Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules.
    Sanger JW
    Cytoskeleton (Hoboken, N.J.) 67.12 (2010 Dec): 808-23.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Sequence and mechanical implications of titin's PEVK region.
    Kumazawa Y
    Advances in experimental medicine and biology 481. (2000): 53-63; discussion 64-6, 107-10.

    Inhibition of nebulin and connectin (titin) for assembly of actin filaments during myofibrillogenesis.
    Shimada Y
    Tissue and cell 32.3 (2000 Jun): 223-7.

    Developmental relationship of myosin binding proteins (myomesin, connectin and C-protein) to myosin in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell structure and function 25.3 (2000 Jun): 177-85.

    Relation of nebulin and connectin (titin) to dynamics of actin in nascent myofibrils of cultured skeletal muscle cells.
    Shimada Y
    Experimental cell research 252.1 (1999 Oct 10): 33-40.

    Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts.
    Field LJ
    The Journal of clinical investigation 98.1 (1996 Jul 1): 216-24.

    Skeletal muscle myofibrillogenesis as revealed with a monoclonal antibody to titin in combination with detection of the alpha- and gamma-isoforms of actin.
    Lessard JL
    Developmental biology 132.1 (1989 Mar): 35-44.

    Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin.
    Lessard JL
    The Journal of cell biology 107.3 (1988 Sep): 1075-83.

    Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.
    Pourquié O
    Nature biotechnology 33.9 (2015 Sep): 962-9.

    Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Pourquié O
    Nature protocols 11.10 (2016 Oct): 1833-50.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    WB References
    IHC References
    Epitope Map References

    PEVK extension of human soleus muscle titin revealed by immunolabeling with the anti-titin antibody 9D10.
    Granzier H
    Journal of structural biology 122.1-2 (1998): 188-96.

    Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils.
    Greaser ML
    Journal of muscle research and cell motility 6.3 (1985 Jun): 293-312.

    Sequence and mechanical implications of titin's PEVK region.
    Kumazawa Y
    Advances in experimental medicine and biology 481. (2000): 53-63; discussion 64-6, 107-10.

    Titin is a target of matrix metalloproteinase-2: implications in myocardial ischemia/reperfusion injury.
    Schulz R
    Circulation 122.20 (2010 Nov 16): 2039-47.

    All References

    Left ventricular torsional mechanics and myocardial iron load in beta-thalassaemia major: a potential role of titin degradation.
    Cheung YF
    BMC cardiovascular disorders 14. (2014 Apr 12): 49.

    Heterogeneity of myotubes generated by the MyoD and E12 basic helix-loop-helix transcription factors in otherwise non-differentiation growth conditions.
    Parpura V
    Biomaterials 35.7 (2014 Feb): 2188-98.

    Titin is a target of matrix metalloproteinase-2: implications in myocardial ischemia/reperfusion injury.
    Schulz R
    Circulation 122.20 (2010 Nov 16): 2039-47.

    Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils.
    Greaser ML
    Journal of muscle research and cell motility 6.3 (1985 Jun): 293-312.

    Arginylation regulates myofibrils to maintain heart function and prevent dilated cardiomyopathy.
    Kashina A
    Journal of molecular and cellular cardiology 53.3 (2012 Sep): 333-41.

    Rearrangement of intercellular junctions and cytoskeletal proteins during rabbit myocardium development.
    Viebahn C
    European journal of cell biology 68.1 (1995 Sep): 62-9.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging.
    Gregorio CC
    Methods (San Diego, Calif.) 66.3 (2014 Apr 1): 370-9.

    Critical roles for multiple formins during cardiac myofibril development and repair.
    Goode BL
    Molecular biology of the cell 25.6 (2014 Mar): 811-27.

    DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila.
    Mihály J
    PLoS genetics 10.2 (2014 Feb): e1004166.

    Epicardium is required for sarcomeric maturation and cardiomyocyte growth in the ventricular compact layer mediated by transforming growth factor β and fibroblast growth factor before the onset of coronary circulation.
    Nakajima Y
    Congenital anomalies 54.3 (2014 Aug): 162-71.

    Pathways of Ca²⁺ entry and cytoskeletal damage following eccentric contractions in mouse skeletal muscle.
    Allen DG
    Journal of applied physiology (Bethesda, Md. : 1985) 112.12 (2012 Jun): 2077-86.

    Comparison of cardiomyogenic potential among human ESC and iPSC lines.
    Lough JW
    Cell transplantation 21.11 (2012): 2523-30.

    Transcription factor rational design improves directed differentiation of human mesenchymal stem cells into skeletal myocytes.
    de Vries AA
    Molecular therapy : the journal of the American Society of Gene Therapy 19.7 (2011 Jul): 1331-41.

    Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload.
    Ross RS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4252-64.

    A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation.
    Martin U
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 463-73.

    Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules.
    Sanger JW
    Cytoskeleton (Hoboken, N.J.) 67.12 (2010 Dec): 808-23.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Sequence and mechanical implications of titin's PEVK region.
    Kumazawa Y
    Advances in experimental medicine and biology 481. (2000): 53-63; discussion 64-6, 107-10.

    Inhibition of nebulin and connectin (titin) for assembly of actin filaments during myofibrillogenesis.
    Shimada Y
    Tissue and cell 32.3 (2000 Jun): 223-7.

    Developmental relationship of myosin binding proteins (myomesin, connectin and C-protein) to myosin in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell structure and function 25.3 (2000 Jun): 177-85.

    Relation of nebulin and connectin (titin) to dynamics of actin in nascent myofibrils of cultured skeletal muscle cells.
    Shimada Y
    Experimental cell research 252.1 (1999 Oct 10): 33-40.

    Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts.
    Field LJ
    The Journal of clinical investigation 98.1 (1996 Jul 1): 216-24.

    Skeletal muscle myofibrillogenesis as revealed with a monoclonal antibody to titin in combination with detection of the alpha- and gamma-isoforms of actin.
    Lessard JL
    Developmental biology 132.1 (1989 Mar): 35-44.

    Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin.
    Lessard JL
    The Journal of cell biology 107.3 (1988 Sep): 1075-83.

    Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.
    Pourquié O
    Nature biotechnology 33.9 (2015 Sep): 962-9.

    Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Pourquié O
    Nature protocols 11.10 (2016 Oct): 1833-50.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    S-glutathionylation of cryptic cysteines enhances titin elasticity by blocking protein folding.
    Fernández JM
    Cell 156.6 (2014 Mar 13): 1235-1246.

    Titin-based stiffening of muscle fibers in Ehlers-Danlos Syndrome.
    Granzier H
    Journal of applied physiology (Bethesda, Md. : 1985) 112.7 (2012 Apr): 1157-65.

    [Expression of titin in the myocardium of spontaneously hypertensive rats during in the course of hypertrophy].
    Podlubnaia ZA
    Biofizika 55.4 (2010 Jul-Aug): 612-8.

    Fast skeletal muscle troponin activation increases force of mouse fast skeletal muscle and ameliorates weakness due to nebulin-deficiency.
    Granzier H
    PloS one 8.2 (2013): e55861.

    PEVK extension of human soleus muscle titin revealed by immunolabeling with the anti-titin antibody 9D10.
    Granzier H
    Journal of structural biology 122.1-2 (1998): 188-96.

    Development of connectin/titin and nebulin in striated muscles of chicken.
    Maruyama K
    Advances in biophysics 33. (1996): 223-33.

    Cultured pig rhabdomyosarcoma cells with a deletion of the Xq24-qter chromosome region: an immunochemical and cytogenetic characterization.
    Ramaekers FC
    American journal of veterinary research 56.8 (1995 Aug): 1062-9.

    Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins.
    Ramaekers FC
    Differentiation; research in biological diversity 46.2 (1991 Mar): 105-15.

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