MANDRA1(7A10)

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SKU: MANDRA1(7A10)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: MANDRA1(7A10)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: DMD
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2618143 
Uniprot ID: P11532 
RRID:  
Entrez Gene ID: 1756 
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: dystrophin
Epitope Location or Sequence: ex77,aa-PSS by phage-display
Alternate Antigen Name:
Deposit Date: 10/26/2005
Antigen Molecular Weight:
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: human
Depositor Notes: This antibody does not work with torpedo
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bear, Fish, Human, Mouse, Xenopus
Additional Information: RRID:AB_2618143
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDRA1(7A10) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDRA1(7A10))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle.
    Lira VA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33.11 (2019 Nov): 11735-11745.

    WB References
    IHC References
    All References

    Skeletal muscles of hibernating black bears show minimal atrophy and phenotype shifting despite prolonged physical inactivity and starvation.
    Tsubota T
    PloS one 14.4 (2019): e0215489.

    ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle.
    Lira VA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33.11 (2019 Nov): 11735-11745.

    Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells.
    Pinset C
    Skeletal muscle 5. (2015): 40.

    Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections.
    Morris GE
    The Biochemical journal 288 ( Pt 2).Pt 2 (1992 Dec 1): 663-8.

    An epitope structure for the C-terminal domain of dystrophin and utrophin.
    Nguyen thi Man
    Biochemistry 37.31 (1998 Aug 4): 11117-27.

    The N-terminal half of dystrophin is protected from proteolysis in situ.
    Morris GE
    Biochemical and biophysical research communications 209.3 (1995 Apr 26): 1062-7.

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