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SKU: 39.3F7
View product citations for antibody 39.3F7 on CiteAb

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Available: 171

DSHB Data Sheet

Catalog Fields

Antigen: Islet-1 specific homeobox
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Fish, Frog, Mouse, Rat, Zebrafish
Depositors Notes: Islet-1 specific ; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006)
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Isl1
Immunogen: Islet-I (rat) C-terminus
Alternate Gene Names: ISL1, ISL LIM homeobox 1
Alternate Antibody Name: anti-islet1; anti-isl1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P61374 
Immunogen Sequence: amino acid residues 178- 349
Entrez Gene ID: 64444 
Additional Characterization:  
Antibody Registry ID: AB_1157901 
Additional Information: The antibody pufiication procedure and the descrition of the plasmid is described in 1764243
Recommended Applications: FACS, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39.3F7 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 39.3F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IHC References
  • FACS References
  • Gel Supershift References
  • All References
  • Initial Publication

    Opticin binds retinal growth hormone in the embryonic vitreous.
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    IHC References
    FACS References

    Precardiac organoids form two heart fields via Bmp/Wnt signaling.
    Kwon C
    Nature communications 9.1 (2018 Aug 7): 3140.

    Gel Supershift References
    All References

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