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$45.00 to $425.00
SKU: 39.3F7
View product citations for antibody 39.3F7 on CiteAb

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Available: 123

DSHB Data Sheet

Catalog Fields

Antigen: Islet-1 specific homeobox
Available to For-Profits: Yes
Antigen Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Jessell, T.M. / Brenner-Morton, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chicken, Fish, Frog, Mouse, Rat, Zebrafish
Depositors Institution: Columbia University / HHMI
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Depositors Notes: Islet-1 specific ; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006)
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Islet-I (rat) C-terminus
Gene: Isl1
Alternate Antibody Name: anti-islet1; anti-isl1
Alternate Gene Names: ISL1, ISL LIM homeobox 1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: No
Uniprot ID: P61374 
Epitope Location or Sequence:
Entrez Gene ID: 64444 
Immunogen Sequence: amino acid residues 178- 349
Antibody Registry ID: AB_1157901 
Additional Characterization:  
Recommended Applications: FACS, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: The antibody pufiication procedure and the descrition of the plasmid is described in 1764243
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39.3F7 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 39.3F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IHC References
  • FACS References
  • Gel Supershift References
  • All References
  • Initial Publication

    Opticin binds retinal growth hormone in the embryonic vitreous.
    Harvey S
    Investigative ophthalmology & visual science 44.12 (2003 Dec): 5404-9.

    IHC References
    FACS References

    Precardiac organoids form two heart fields via Bmp/Wnt signaling.
    Kwon C
    Nature communications 9.1 (2018 Aug 7): 3140.

    Gel Supershift References
    All References

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