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SKU: 39.3F7
View product citations for antibody 39.3F7 on CiteAb

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Available: 204

DSHB Data Sheet

Catalog Fields

Antigen: Islet-1 specific homeobox
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Fish, Frog, Mouse, Rat, Zebrafish
Depositors Notes: Islet-1 specific ; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006)
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Isl1
Immunogen: Islet-I (rat) C-terminus
Alternate Gene Names: ISL1, ISL LIM homeobox 1
Alternate Antibody Name: anti-islet1; anti-isl1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P61374 
Immunogen Sequence: amino acid residues 178- 349
Entrez Gene ID: 64444 
Additional Characterization:
Antibody Registry ID: AB_1157901 
Additional Information: The antibody pufiication procedure and the descrition of the plasmid is described in 1764243
Recommended Applications: FACS, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39.3F7 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 39.3F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IHC References
  • FACS References
  • Gel Supershift References
  • All References
  • Initial Publication

    Opticin binds retinal growth hormone in the embryonic vitreous.
    Harvey S
    Investigative ophthalmology and visual science 44.12 (2003 Dec): 5404-9.

    IHC References
    FACS References

    Precardiac organoids form two heart fields via Bmp/Wnt signaling.
    Kwon C
    Nature communications 9.1 (2018 Aug 7): 3140.

    Gel Supershift References
    All References

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