67.4E12

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$40.00
SKU: 67.4E12
View product citations for antibody 67.4E12 on CiteAb

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Available: 188

DSHB Data Sheet

Catalog Fields

Antigen: Lim3
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence: full length protein
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rodent
Depositors Notes:
Antigen Molecular Weight: 44kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lim-3
Immunogen: full length cDNA/GST fusion
Alternate Gene Names: LIM homeobox protein 3,Plim, Lim-3,Lim3; Lhx3,Lim3b, MUSLIM3B, Lim homeobox gene 3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P50481 
Immunogen Sequence: full length cDNA GST Fusion
Entrez Gene ID: 16871 
Additional Characterization:
Antibody Registry ID: AB_2135805 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
67.4E12 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 67.4E12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    IHC References

    Newborn horizontal cells migrate bi-directionally across the neuroepithelium during retinal development.
    Hallböök F
    Development (Cambridge, England) 131.6 (2004 Mar): 1343-51.

    Early identification of retinal subtypes in the developing, pre-laminated chick retina using the transcription factors Prox1, Lim1, Ap2alpha, Pax6, Isl1, Isl2, Lim3 and Chx10.
    Hallböök F
    European journal of histochemistry : EJH 50.2 (2006 Apr-Jun): 147-54.

    Transient expression of LIM-domain transcription factors is coincident with delayed maturation of photoreceptors in the chicken retina.
    Sherwood P
    The Journal of comparative neurology 506.4 (2008 Feb 1): 584-603.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    Crossregulation between Neurogenin2 and pathways specifying neuronal identity in the spinal cord.
    Guillemot F
    Neuron 31.2 (2001 Aug 2): 203-17.

    Suppression of interneuron programs and maintenance of selected spinal motor neuron fates by the transcription factor AML1/Runx1.
    Stifani S
    Proceedings of the National Academy of Sciences of the United States of America 105.17 (2008 Apr 29): 6451-6.

    Motoneuronal Sema3C is essential for setting stereotyped motor tract positioning in limb-derived chemotropic semaphorins.
    Castellani V
    Development (Cambridge, England) 139.19 (2012 Oct): 3633-43.

    Onecut transcription factors act upstream of Isl1 to regulate spinal motoneuron diversification.
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Proliferation and recapitulation of developmental patterning associated with regulative regeneration of the spinal cord neural tube.
    Glover JC
    Developmental biology 365.1 (2012 May 1): 118-32.

    Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle.
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    The maturation of photoreceptors in the avian retina is stimulated by thyroid hormone.
    Sherwood P
    Neuroscience 178. (2011 Mar 31): 250-60.

    Quinine inhibits multiple Na+ and K+ transport mechanisms in Ehrlich ascites tumor cells.
    Levinson C
    Biochimica et biophysica acta 978.1 (1989 Jan 16): 169-75.

    Fibronectin-enhanced phagocytosis of an alternative pathway activator by human culture-derived macrophages is mediated by the C4b/C3b complement receptor (CR1).
    Brown EJ
    Journal of immunology (Baltimore, Md. : 1950) 135.4 (1985 Oct): 2680-6.

    The transcription factors Nkx2.2 and Nkx2.9 play a novel role in floor plate development and commissural axon guidance.
    Arnold HH
    Development (Cambridge, England) 137.24 (2010 Dec): 4249-60.

    Receptor tyrosine phosphatase PTPγ is a regulator of spinal cord neurogenesis.
    Stoker AW
    Molecular and cellular neurosciences 46.2 (2011 Feb): 469-82.

    Comparative functional genomics revealed conservation and diversification of three enhancers of the isl1 gene for motor and sensory neuron-specific expression.
    Okamoto H
    Developmental biology 278.2 (2005 Feb 15): 587-606.

    IP References
    FFPE References
    All References

    Newborn horizontal cells migrate bi-directionally across the neuroepithelium during retinal development.
    Hallböök F
    Development (Cambridge, England) 131.6 (2004 Mar): 1343-51.

    Early identification of retinal subtypes in the developing, pre-laminated chick retina using the transcription factors Prox1, Lim1, Ap2alpha, Pax6, Isl1, Isl2, Lim3 and Chx10.
    Hallböök F
    European journal of histochemistry : EJH 50.2 (2006 Apr-Jun): 147-54.

    Transient expression of LIM-domain transcription factors is coincident with delayed maturation of photoreceptors in the chicken retina.
    Sherwood P
    The Journal of comparative neurology 506.4 (2008 Feb 1): 584-603.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    Crossregulation between Neurogenin2 and pathways specifying neuronal identity in the spinal cord.
    Guillemot F
    Neuron 31.2 (2001 Aug 2): 203-17.

    Suppression of interneuron programs and maintenance of selected spinal motor neuron fates by the transcription factor AML1/Runx1.
    Stifani S
    Proceedings of the National Academy of Sciences of the United States of America 105.17 (2008 Apr 29): 6451-6.

    Motoneuronal Sema3C is essential for setting stereotyped motor tract positioning in limb-derived chemotropic semaphorins.
    Castellani V
    Development (Cambridge, England) 139.19 (2012 Oct): 3633-43.

    Onecut transcription factors act upstream of Isl1 to regulate spinal motoneuron diversification.
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Proliferation and recapitulation of developmental patterning associated with regulative regeneration of the spinal cord neural tube.
    Glover JC
    Developmental biology 365.1 (2012 May 1): 118-32.

    Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle.
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    The maturation of photoreceptors in the avian retina is stimulated by thyroid hormone.
    Sherwood P
    Neuroscience 178. (2011 Mar 31): 250-60.

    Quinine inhibits multiple Na+ and K+ transport mechanisms in Ehrlich ascites tumor cells.
    Levinson C
    Biochimica et biophysica acta 978.1 (1989 Jan 16): 169-75.

    Fibronectin-enhanced phagocytosis of an alternative pathway activator by human culture-derived macrophages is mediated by the C4b/C3b complement receptor (CR1).
    Brown EJ
    Journal of immunology (Baltimore, Md. : 1950) 135.4 (1985 Oct): 2680-6.

    The transcription factors Nkx2.2 and Nkx2.9 play a novel role in floor plate development and commissural axon guidance.
    Arnold HH
    Development (Cambridge, England) 137.24 (2010 Dec): 4249-60.

    Receptor tyrosine phosphatase PTPγ is a regulator of spinal cord neurogenesis.
    Stoker AW
    Molecular and cellular neurosciences 46.2 (2011 Feb): 469-82.

    Comparative functional genomics revealed conservation and diversification of three enhancers of the isl1 gene for motor and sensory neuron-specific expression.
    Okamoto H
    Developmental biology 278.2 (2005 Feb 15): 587-606.

    Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development.
    Clotman F
    Neuroscience 165.1 (2010 Jan 13): 116-29.

    Canonical WNT Signaling Regulates the Pituitary Organizer and Pituitary Gland Formation.
    Davis SW
    Endocrinology 158.10 (2017 Oct 1): 3339-3353.

    LHX3 interacts with inhibitor of histone acetyltransferase complex subunits LANP and TAF-1β to modulate pituitary gene regulation.
    Rhodes SJ
    PloS one 8.7 (2013): e68898.

    Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling.
    Briscoe J
    Cell 90.1 (1997 Jul 11): 169-80.

    Expression pattern of the murine LIM class homeobox gene Lhx3 in subsets of neural and neuroendocrine tissues.
    Westphal H
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 354-64.

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