67.4E12

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$40.00
SKU: 67.4E12
View product citations for antibody 67.4E12 on CiteAb

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Available: 181

DSHB Data Sheet

Catalog Fields

Antigen: Lim3
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence: full length protein
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rodent
Depositors Notes:
Antigen Molecular Weight: 44kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lim-3
Immunogen: full length cDNA/GST fusion
Alternate Gene Names: LIM homeobox protein 3,Plim, Lim-3,Lim3; Lhx3,Lim3b, MUSLIM3B, Lim homeobox gene 3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P50481 
Immunogen Sequence: full length cDNA GST Fusion
Entrez Gene ID: 16871 
Additional Characterization:
Antibody Registry ID: AB_2135805 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
67.4E12 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 67.4E12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication

    Cell
    Briscoe J
    Cell 90.1 (1997 Jul 11): 169-80.

    IF References

    Neuroscience
    Clotman F
    Neuroscience 165.1 (2010 Jan 13): 116-29.

    Endocrinology
    Davis SW
    Endocrinology 158.10 (2017 Oct 1): 3339-3353.

    IHC References

    Development (Cambridge, England)
    Hallböök F
    Development (Cambridge, England) 131.6 (2004 Mar): 1343-51.

    European journal of histochemistry : EJH
    Hallböök F
    European journal of histochemistry : EJH 50.2 (2006 Apr-Jun): 147-54.

    The Journal of comparative neurology
    Sherwood P
    The Journal of comparative neurology 506.4 (2008 Feb 1): 584-603.

    PloS one
    Luo J
    PloS one 9.1 (2014): e84617.

    Neuron
    Guillemot F
    Neuron 31.2 (2001 Aug 2): 203-17.

    Proceedings of the National Academy of Sciences of the United States of America
    Stifani S
    Proceedings of the National Academy of Sciences of the United States of America 105.17 (2008 Apr 29): 6451-6.

    Development (Cambridge, England)
    Castellani V
    Development (Cambridge, England) 139.19 (2012 Oct): 3633-43.

    Development (Cambridge, England)
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Developmental biology
    Glover JC
    Developmental biology 365.1 (2012 May 1): 118-32.

    Stem cell research
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    Neuroscience
    Sherwood P
    Neuroscience 178. (2011 Mar 31): 250-60.

    Biochimica et biophysica acta
    Levinson C
    Biochimica et biophysica acta 978.1 (1989 Jan 16): 169-75.

    Journal of immunology (Baltimore, Md. : 1950)
    Brown EJ
    Journal of immunology (Baltimore, Md. : 1950) 135.4 (1985 Oct): 2680-6.

    Development (Cambridge, England)
    Arnold HH
    Development (Cambridge, England) 137.24 (2010 Dec): 4249-60.

    Molecular and cellular neurosciences
    Stoker AW
    Molecular and cellular neurosciences 46.2 (2011 Feb): 469-82.

    Developmental biology
    Okamoto H
    Developmental biology 278.2 (2005 Feb 15): 587-606.

    IP References

    PloS one
    Rhodes SJ
    PloS one 8.7 (2013): e68898.

    FFPE References

    Endocrinology
    Davis SW
    Endocrinology 158.10 (2017 Oct 1): 3339-3353.

    All References

    Development (Cambridge, England)
    Hallböök F
    Development (Cambridge, England) 131.6 (2004 Mar): 1343-51.

    European journal of histochemistry : EJH
    Hallböök F
    European journal of histochemistry : EJH 50.2 (2006 Apr-Jun): 147-54.

    The Journal of comparative neurology
    Sherwood P
    The Journal of comparative neurology 506.4 (2008 Feb 1): 584-603.

    PloS one
    Luo J
    PloS one 9.1 (2014): e84617.

    Neuron
    Guillemot F
    Neuron 31.2 (2001 Aug 2): 203-17.

    Proceedings of the National Academy of Sciences of the United States of America
    Stifani S
    Proceedings of the National Academy of Sciences of the United States of America 105.17 (2008 Apr 29): 6451-6.

    Development (Cambridge, England)
    Castellani V
    Development (Cambridge, England) 139.19 (2012 Oct): 3633-43.

    Development (Cambridge, England)
    Clotman F
    Development (Cambridge, England) 139.17 (2012 Sep): 3109-19.

    Developmental biology
    Glover JC
    Developmental biology 365.1 (2012 May 1): 118-32.

    Stem cell research
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    Neuroscience
    Sherwood P
    Neuroscience 178. (2011 Mar 31): 250-60.

    Biochimica et biophysica acta
    Levinson C
    Biochimica et biophysica acta 978.1 (1989 Jan 16): 169-75.

    Journal of immunology (Baltimore, Md. : 1950)
    Brown EJ
    Journal of immunology (Baltimore, Md. : 1950) 135.4 (1985 Oct): 2680-6.

    Development (Cambridge, England)
    Arnold HH
    Development (Cambridge, England) 137.24 (2010 Dec): 4249-60.

    Molecular and cellular neurosciences
    Stoker AW
    Molecular and cellular neurosciences 46.2 (2011 Feb): 469-82.

    Developmental biology
    Okamoto H
    Developmental biology 278.2 (2005 Feb 15): 587-606.

    Neuroscience
    Clotman F
    Neuroscience 165.1 (2010 Jan 13): 116-29.

    Endocrinology
    Davis SW
    Endocrinology 158.10 (2017 Oct 1): 3339-3353.

    PloS one
    Rhodes SJ
    PloS one 8.7 (2013): e68898.

    Cell
    Briscoe J
    Cell 90.1 (1997 Jul 11): 169-80.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Westphal H
    Developmental dynamics : an official publication of the American Association of Anatomists 202.4 (1995 Apr): 354-64.

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