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SKU: 4G11
View product citations for antibody 4G11 on CiteAb

In Stock

Available: 77

DSHB Data Sheet

Catalog Fields

Antigen: Engrail-1
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Fish, Zebrafish
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: EN1
Immunogen: peptide
Alternate Gene Names: Gg-En1/En-1/en-3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q05916 
Immunogen Sequence: NFFIDNILRPDFG
Entrez Gene ID: 771008 
Additional Characterization:
Antibody Registry ID: AB_2314371 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4G11 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 4G11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

    Foxa1 and Foxa2 regulate multiple phases of midbrain dopaminergic neuron development in a dosage-dependent manner.
    Ang SL
    Development (Cambridge, England) 134.15 (2007 Aug): 2761-9.

    The apical ectodermal ridge (AER) can be re-induced by wounding, wnt-2b, and fgf-10 in the chicken limb bud.
    Wada N
    Developmental biology 342.2 (2010 Jun 15): 157-68.

    Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
    Volovitch M
    Neural development 2. (2007 Jan 17): 2.

    Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling.
    Briscoe J
    Cell 90.1 (1997 Jul 11): 169-80.

    Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo.
    Prochiantz A
    Neuron 64.3 (2009 Nov 12): 355-366.

    Long-term survival of dopamine neurons derived from parthenogenetic primate embryonic stem cells (cyno-1) after transplantation.
    Isacson O
    Stem cells (Dayton, Ohio) 23.7 (2005 Aug): 914-22.

    Genetic selection of sox1GFP-expressing neural precursors removes residual tumorigenic pluripotent stem cells and attenuates tumor formation after transplantation.
    Kim KS
    Journal of neurochemistry 97.5 (2006 Jun): 1467-80.

    Renshaw cell interneuron specialization is controlled by a temporally restricted transcription factor program.
    Goulding M
    Development (Cambridge, England) 139.1 (2012 Jan): 179-90.

    Identification of embryonic stem cell-derived midbrain dopaminergic neurons for engraftment.
    Studer L
    The Journal of clinical investigation 122.8 (2012 Aug): 2928-39.

    Identification of cerebellin2 in chick and its preferential expression by subsets of developing sensory neurons and their targets in the dorsal horn.
    Honig MG
    The Journal of comparative neurology 518.14 (2010 Jul 15): 2818-40.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

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