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SKU: 4G11

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Available: 222

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 4G11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: EN1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528219 
Uniprot ID: Q05916 
Entrez Gene ID: 771008 
Clonality: Monoclonal
Immunogen: peptide
Immunogen Sequence: NFFIDNILRPDFG
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: Engrail-1
Epitope Location or Sequence: a.a. 69-81
Alternate Antigen Name:
Deposit Date: 7/9/1997
Antigen Molecular Weight:
Depositor: Jessell, T.M. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University / HHMI
Antigen Species: Chicken
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Fish, Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4G11 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 4G11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

12 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

    Foxa1 and Foxa2 regulate multiple phases of midbrain dopaminergic neuron development in a dosage-dependent manner.
    Ang SL
    Development (Cambridge, England) 134.15 (2007 Aug): 2761-9.

    The apical ectodermal ridge (AER) can be re-induced by wounding, wnt-2b, and fgf-10 in the chicken limb bud.
    Wada N
    Developmental biology 342.2 (2010 Jun 15): 157-68.

    Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
    Volovitch M
    Neural development 2. (2007 Jan 17): 2.

    Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling.
    Briscoe J
    Cell 90.1 (1997 Jul 11): 169-80.

    Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo.
    Prochiantz A
    Neuron 64.3 (2009 Nov 12): 355-366.

    Long-term survival of dopamine neurons derived from parthenogenetic primate embryonic stem cells (cyno-1) after transplantation.
    Isacson O
    Stem cells (Dayton, Ohio) 23.7 (2005 Aug): 914-22.

    Genetic selection of sox1GFP-expressing neural precursors removes residual tumorigenic pluripotent stem cells and attenuates tumor formation after transplantation.
    Kim KS
    Journal of neurochemistry 97.5 (2006 Jun): 1467-80.

    Renshaw cell interneuron specialization is controlled by a temporally restricted transcription factor program.
    Goulding M
    Development (Cambridge, England) 139.1 (2012 Jan): 179-90.

    Identification of embryonic stem cell-derived midbrain dopaminergic neurons for engraftment.
    Studer L
    The Journal of clinical investigation 122.8 (2012 Aug): 2928-39.

    Identification of cerebellin2 in chick and its preferential expression by subsets of developing sensory neurons and their targets in the dorsal horn.
    Honig MG
    The Journal of comparative neurology 518.14 (2010 Jul 15): 2818-40.

    Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use.
    Studer L
    Cell stem cell 28.2 (2021 Feb 4): 343-355.e5.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

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