4H6

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SKU: 4H6

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 4H6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: NEFM
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528400 
Uniprot ID: P16053 
RRID:  
Entrez Gene ID: 396206 
Clonality: Monoclonal
Immunogen: Chicken optic nerve (Developmental Stage: E10)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63Ag8.653
Epitope Mapped: No
Antigen Name: neurofilament (NF-M)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/8/2001
Antigen Molecular Weight: Predicted: 96 kDa; Apparent: doublet at 160 kDa
Depositor: Halfter, W.M.
Antigen Sequence:
Depositor Institution: University of Pittsburgh
Antigen Species: chicken
Depositor Notes: Avian specific.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information: Neuronal cell marker.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4H6 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 4H6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Axonin 1 is expressed primarily in subclasses of avian sensory neurons during outgrowth.
    Yip JW
    Brain research. Developmental brain research 78.1 (1994 Mar 18): 87-101.

    IF References

    Axonin 1 is expressed primarily in subclasses of avian sensory neurons during outgrowth.
    Yip JW
    Brain research. Developmental brain research 78.1 (1994 Mar 18): 87-101.

    Pelvic plexus contributes ganglion cells to the hindgut enteric nervous system.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 236.1 (2007 Jan): 73-83.

    HSPB3 protein is expressed in motoneurons and induces their survival after lesion-induced degeneration.
    Krieglstein K
    Experimental neurology 286. (2016 Dec): 40-49.

    Simple explant culture of the embryonic chicken retina with long-term preservation of photoreceptors.
    Layer PG
    Experimental eye research 93.4 (2011 Oct): 556-64.

    Reprogramming retinal pigment epithelium to differentiate toward retinal neurons with Sox2.
    Wang SZ
    Stem cells (Dayton, Ohio) 27.6 (2009 Jun): 1376-87.

    The chick optic tectum developmental stages. A dynamic table based on temporal- and spatial-dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis.
    Flores V
    Journal of morphology 272.6 (2011 Jun): 675-97.

    Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development.
    Lin J
    Journal of molecular neuroscience : MN 64.2 (2018 Feb): 287-299.

    WB References

    Axonin 1 is expressed primarily in subclasses of avian sensory neurons during outgrowth.
    Yip JW
    Brain research. Developmental brain research 78.1 (1994 Mar 18): 87-101.

    IHC References

    Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric nervous system.
    Goldstein AM
    Developmental biology 293.1 (2006 May 1): 203-17.

    Intestinal coelomic transplants: a novel method for studying enteric nervous system development.
    Goldstein AM
    Cell and tissue research 326.1 (2006 Oct): 43-55.

    Pelvic plexus contributes ganglion cells to the hindgut enteric nervous system.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 236.1 (2007 Jan): 73-83.

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    bHLH genes cath5 and cNSCL1 promote bFGF-stimulated RPE cells to transdifferentiate toward retinal ganglion cells.
    Wang SZ
    Developmental biology 265.2 (2004 Jan 15): 320-8.

    Enhanced retinal ganglion cell differentiation by ath5 and NSCL1 coexpression.
    Wang SZ
    Investigative ophthalmology & visual science 45.9 (2004 Sep): 2922-8.

    All References

    Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric nervous system.
    Goldstein AM
    Developmental biology 293.1 (2006 May 1): 203-17.

    Intestinal coelomic transplants: a novel method for studying enteric nervous system development.
    Goldstein AM
    Cell and tissue research 326.1 (2006 Oct): 43-55.

    Pelvic plexus contributes ganglion cells to the hindgut enteric nervous system.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 236.1 (2007 Jan): 73-83.

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    bHLH genes cath5 and cNSCL1 promote bFGF-stimulated RPE cells to transdifferentiate toward retinal ganglion cells.
    Wang SZ
    Developmental biology 265.2 (2004 Jan 15): 320-8.

    Enhanced retinal ganglion cell differentiation by ath5 and NSCL1 coexpression.
    Wang SZ
    Investigative ophthalmology & visual science 45.9 (2004 Sep): 2922-8.

    Axonin 1 is expressed primarily in subclasses of avian sensory neurons during outgrowth.
    Yip JW
    Brain research. Developmental brain research 78.1 (1994 Mar 18): 87-101.

    HSPB3 protein is expressed in motoneurons and induces their survival after lesion-induced degeneration.
    Krieglstein K
    Experimental neurology 286. (2016 Dec): 40-49.

    Simple explant culture of the embryonic chicken retina with long-term preservation of photoreceptors.
    Layer PG
    Experimental eye research 93.4 (2011 Oct): 556-64.

    Reprogramming retinal pigment epithelium to differentiate toward retinal neurons with Sox2.
    Wang SZ
    Stem cells (Dayton, Ohio) 27.6 (2009 Jun): 1376-87.

    The chick optic tectum developmental stages. A dynamic table based on temporal- and spatial-dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis.
    Flores V
    Journal of morphology 272.6 (2011 Jun): 675-97.

    Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development.
    Lin J
    Journal of molecular neuroscience : MN 64.2 (2018 Feb): 287-299.

    A bird's eye view of enteric nervous system development: lessons from the avian embryo.
    Nagy N
    Pediatric research 64.4 (2008 Oct): 326-33.

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