4H6
Catalog Fields
Antigen: neurofilament (NF-M)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pittsburgh
Positive Tested Species Reactivity: Avian
Depositors Notes: Avian specific.
Predicted Species Reactivity:
Gene: NEFM
Immunogen: Chicken optic nerve (Developmental Stage: E10)
Alternate Gene Names: NEF3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P16053
Immunogen Sequence: Full length protein
Entrez Gene ID: 396206
Antibody Registry ID: AB_528400
Additional Information: Neuronal cell marker.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4H6 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 4H6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Axonin 1 is expressed primarily in subclasses of avian sensory neurons during outgrowth.
Yip JW
Brain research. Developmental brain research 78.1 (1994 Mar 18): 87-101.