6G7

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SKU: 6G7

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 6G7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: TUBB3
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528497 
Uniprot ID: P09206 
RRID:  
Entrez Gene ID: 431043 
Clonality: Monoclonal
Immunogen: Retina (chick) embryonic day E6.
Clone:
Immunogen Sequence:
Myeloma Strain: Ag8
Epitope Mapped: No
Antigen Name: Tubulin (beta III)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/30/2004
Antigen Molecular Weight: 49.8 kDa
Depositor: Halfter, W.M.
Antigen Sequence: Full length protein
Depositor Institution: University of Pittsburgh
Antigen Species: Chicken
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans, Chicken, Fish, Human, Mouse, Quail, Xenopus, Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6G7 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 6G7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A critical function of the pial basement membrane in cortical histogenesis.
    Mayer U
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.14 (2002 Jul 15): 6029-40.

    IF References

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons.
    Amara SG
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.8 (2013 Aug): 2995-3007.

    WB References
    All References

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons.
    Amara SG
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.8 (2013 Aug): 2995-3007.

    The potassium channel, Kir3.4 participates in angiotensin II-stimulated aldosterone production by a human adrenocortical cell line.
    Gomez-Sanchez CE
    Endocrinology 153.9 (2012 Sep): 4328-35.

    The bi-functional organization of human basement membranes.
    Henrich PB
    PloS one 8.7 (2013): e67660.

    SPG20 protein spartin associates with cardiolipin via its plant-related senescence domain and regulates mitochondrial Ca2+ homeostasis.
    Bakowska JC
    PloS one 6.4 (2011 Apr 29): e19290.

    Polarized deposition of basement membrane proteins depends on Phosphatidylinositol synthase and the levels of Phosphatidylinositol 4,5-bisphosphate.
    Schüpbach T
    Proceedings of the National Academy of Sciences of the United States of America 111.21 (2014 May 27): 7689-94.

    A critical function of the pial basement membrane in cortical histogenesis.
    Mayer U
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.14 (2002 Jul 15): 6029-40.

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